AV F404

Submitted by Andreas Borchel on 2 May, 2019 - 09:49

Phenotype CV:

General

Experiment Batch ID:

AV

Experiment Contact:

Andreas Borchel

Start Date:

14 March, 2019 - 08:35

End Date:

24 April, 2019 - 08:23

Experiment Description:

injection into pradult females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult1

Adult

Target description

Primary target ID:

EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment length:

400bp

Fragment concentration:

600ng/ul

Sense sequence:

TGTTGGCCCTATGAGTCCATCT TTTTTGTGACTTTGATGCCTTGA

Antisense sequence:

TGATCCTTGGAAGAAAAGAGGATG TTCATCATTTCCAGCATTGAGTTT

Fragment Label ID:

F404

Fragment Mixture Formula:

50% of both, in total 600 ng/ul

Fragment Producer:

licelab

Fragment Producer Contact:

Andreas Borchel

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

15

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Knockdown of a testis-specific ion-pump in pre-adult1 males, checking reproduction in adults. Many males were lost, and no kncokdown observed.

AS F384

Submitted by Andreas Borchel on 21 November, 2018 - 11:10

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis

EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment concentration:

600ng/ul

Fragment Batch ID:

F384

Fragment Label ID:

F384

Fragment Mixture Formula:

F381+F382+F383 in same ratio

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

21

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knockdown lead to missing egg strings

AS F380

Submitted by Andreas Borchel on 21 November, 2018 - 09:59

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000000454, EMLSAT00000000454-696301 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000000454

Fragment description

Fragment length:

500bp

Fragment concentration:

600ng/ul

Sense sequence:

GTCCACTCGTATGCCCACCTCTCTGTGAGGAACATGAAATGTTTTGTCCCGGATCCATGGTTGATGAATGTCCTACAGAAGATAATTGCGTTGCCAAAATTGATGAAAATAACAAGTGTCCAAATTATTGTCCAATTACTTGTGAAAAAGGTCAACACATATGTCCTGGAATCCGAAAAGATGATGGATGCAGAGGTCCAGATACCTGTGTACCCATTGGTACACAATGTCCAAGTGAGATTTTTGATGAATTTGCTTGTCCCATCATTGATTCACCCAGTTGTGTGTATCCCGAAACTCATTGCCATGGTGGTTACAATTCAAGGGGTTGTCGAAAACGATCCTTCTGTAAGGAATTAAAGAATAAAACAGAGCATCAACATGGTGTAAATGGCATTGATGGTTGTCCAACATTTTGTCCAGCAACATGCATTCCAGGTATTGAGACAGAATGCCCTGAAACCTACGATGAAGATGGATGTCCTCTTCCTGCGTCTTGC

Fragment Batch ID:

F380

Fragment Label ID:

F380

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

20

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knockdown lead to missing egg strings

AS F379

Submitted by Andreas Borchel on 21 November, 2018 - 09:46

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000008518

Fragment description

Fragment length:

483bp

Fragment concentration:

600ng/ul

Sense sequence:

GGACGGTGTGTGACTCCAAACACCTGCAAGTGTGATTCTGGGTGGAGGGGTGATACCTGCAATGAGTGTATTCCCTCATCTCAGTGTCAAAATGGATTTTGCGTTCATTCTCCGAAGGAATGTATCTGCAGTTTGGGCTGGGCTGGAGTAAACTGTGATCTACAGGATCATGGATGTGTCCATGGAACCTTTTCATCAAAACATCACTGCACATGCGATCCGGGATGGTTAGGAAGAAATTGCAGTTCTTGTATACCGCACCCAAGTTGTGTTTGGGGATCATGTAAATCCACTCCGGGCGAATGTTTTTGTGATCCAGGATGGGATGGAAGGAATTGTGACATCCCCATTTGCGAGAAAGGGTGTCATCCAGATCACGGATTTTGTTTTAAGCCATCTCAATGTATATGTCGCTCTGGTTGGCATGGAAAACGTTGTGATCTTTGCACTCCAATGCCTGGATGCACACGAGGATGCCATAAA

Fragment Batch ID:

F379

Fragment Label ID:

F379

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

15

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knockdown lead to missing egg strings

AP F367 EMLSAG00000005299

Submitted by Christiane Eichner on 29 May, 2018 - 12:27

Phenotype CV:

General

Experiment Batch ID:

AP

Experiment Contact:

Christiane Eichner

Start Date:

6 March, 2018 - 09:00

End Date:

10 April, 2018 - 09:00

Experiment Description:

EMLSAG1458 knock-down

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000005299

Fragment description

Fragment length:

338bp

Fragment concentration:

600ng/ul

Sense sequence:

>F367 gtatgatgacggacatgctcaagggtaacattactaacatgttgccaatgattgtaattggaggatggatcaactggcatttctcaggctttgtcactaccaaaatcccttttcccctcactcttcgtttcaagcctatgcttcaaagaggcattgaactcatgagtctagatgcttcatgggtctcctccgcctcctggtactttcttaacgttttcggactacgatctatttatgacttagttctcggagaaaataatgttgcggatcaatcaagaatcatgcaggaccagatgatgatgcctgccaacggaatggctaccgactataaacaggcc

Fragment Label ID:

F367

Primer Group:

likb2502_T7_b2515

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Eichner

Validation

Efficacy of Knock Down:

F>16%

Phenotype

Number of Individuals (End):

24

Phenotype:

slightly shorter gonade and abdominal segment

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Testing the fragment for Mvp1 knock-down.

Nauplii I 12262+22100+4547+12296+12453

Submitted by Anna Komisarczuk on 2 May, 2018 - 14:43

Phenotype CV:

reproduction

General

Experiment Batch ID:

N

Experiment Contact:

Anna Komisarczuk

Start Date:

1 February, 2016 - 14:42

End Date:

10 February, 2016 - 01:43

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Developmental Stage CV:

Nauplius I

Target description

Primary target ID:

EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis

EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis

EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis

EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis

Fragment description

Fragment Label ID:

F256

Phenotype

Phenotype:

reproductive defect as adults

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