RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
31 AG-F286 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011448, EMLSAT00000011448-707295 (mRNA) Lepeophtheirus salmonis PreAdult2 Slightly curly eggstrings. 29/19 Lepeophtheirus salmonis 16 March, 2017 - 11:15
32 AH F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/21 Lepeophtheirus salmonis 22 March, 2018 - 14:36
33 AH F243 EMLSAG00000005306, EMLSAG00000005306-688072 (gene) Lepeophtheirus salmonis
T4_5306
PreAdult2, Adult No visible phenotype
no visible phenotype
30/17 Lepeophtheirus salmonis 22 March, 2018 - 14:30
34 AI F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:38
35 AJ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:41
36 AL F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30 Lepeophtheirus salmonis 22 March, 2018 - 14:42
37 AL F338 EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis, EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
FTZ-F1
PreAdult2, Adult No eggstrings
female reproduction, no eggstrings
30/12 Lepeophtheirus salmonis F>45.00% 14 June, 2018 - 12:56
38 AL F339 EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis, EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
FTZ-F1
PreAdult2, Adult No eggstrings
female reproduction, no eggstrings
30/7 Lepeophtheirus salmonis F>45.00% 14 June, 2018 - 13:14
39 AL Hormone receptor 3 (HR3) EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
EMLSAG00000000733
PreAdult2 No eggstrings.
female reproduction, no eggstrings
30/17 Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 10:47
40 AM F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30 Lepeophtheirus salmonis 22 March, 2018 - 14:44

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