RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
31 AF F241 EMLSAG00000002443, EMLSAG00000002443-685209 (gene) Lepeophtheirus salmonis
T2_2443 		PreAdult2, Adult no visible phenotype 30/21 Lepeophtheirus salmonis 16 July, 2016 - 12:00
32 AF F263 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis
441, 12943 		PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:51
33 AF F264 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000006733, EMLSAG00000006733-689499 (gene) Lepeophtheirus salmonis
441, 12943, 6733 		PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:56
34 AG F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2, Adult Control 30/15 Lepeophtheirus salmonis 22 March, 2018 - 14:40
35 AG F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No phenotype
no visible phenotype 		30/16 Lepeophtheirus salmonis F>99.90% 21 February, 2017 - 10:15
36 AG-F286 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011448, EMLSAT00000011448-707295 (mRNA) Lepeophtheirus salmonis PreAdult2 Slightly curly eggstrings. 29/19 Lepeophtheirus salmonis 16 March, 2017 - 11:15
37 AH F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2, Adult Control 30/21 Lepeophtheirus salmonis 22 March, 2018 - 14:36
38 AH F242 EMLSAG00000001206, EMLSAG00000001206-683972 (gene) Lepeophtheirus salmonis
T3_1206 		PreAdult2, Adult no visible phenotype 30/19 Lepeophtheirus salmonis 22 March, 2018 - 14:27
39 AH F243 EMLSAG00000005306, EMLSAG00000005306-688072 (gene) Lepeophtheirus salmonis
T4_5306 		PreAdult2, Adult No visible phenotype
no visible phenotype 		30/17 Lepeophtheirus salmonis 22 March, 2018 - 14:30
40 AI F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:38

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