beta FTZ-F1 | LiceBase

Submitted by Joakim Brunet on 14 June, 2018 - 13:50

General

End Date:

Wednesday, January 24, 2018 - 13:00

Experiment Description:

Sampled larvae at middle/old nauplius 2 to measure downregulation before visible phenotype.

Experiment Batch ID:

Experiment bFTZ-F1

Start Date:

Wednesday, January 17, 2018 - 13:00

Experiment Contact:

Joakim Brunet

Sample

Developmental Stage CV:

Nauplius

Copepodid

Sex:

both

Organism:

Lepeophtheirus salmonis

Target description

Gene or target symbol:

beta FTZ-F1

Target type:

mRNA

Primary target ID:

EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis

EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment Producer Contact:

Joakim Brunet

Fragment Label ID:

beta FTZ-F1

Fragment Batch ID:

beta FTZ-F1

Fragment concentration:

10.00ng/ul

Fragment ID:

beta FTZ-F1, beta FTZ-F1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment length:

468bp

Sense sequence:

ttgaggacaacaacgacgactcta Ctatactcattagctatacattgttgtgtggatttcgaaaagagacgcggacgataagga Gcagggaatttgtgatttcgaaaaaaaaatagtaaaaatgtgccatggtggtttgagatg Accaaatacatttataagggcaagttacgtgaaaaaggcccttgaatgttacccacggaa Cctcttattagtattccgtattctaaccaacgtggacgtttccatcaaccccatattcaa Gagctgcctctcgcagccccgggttctaatcatagatcatcatccctgatccttataaaa Aaagagccgaatatgagcacaagtggaagcttgagtagcggtggtagtcctcccatggtt Ggaggaacactcctcccaaccatggatatgccgatcggaaactcatttgcttccttggaa Gacctgagcagtgtagtccatcca

Fragment Producer:

licelab

Validation

Efficacy of Knock Down:

F>50.00%

Phenotype

Phenotype CV:

Phenotype:

Developmental arrest

Number of Individuals (End):

Gallery Image:

Image file:

bFTZF1_31.5X_2018_01_24_9_6.JPG

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Soaking of larvae with dsRNA specific for the transcript variant beta for the gene FTZ-F1. Resulted in developmental arrest.

End Date:

Experiment Description:

Experiment Batch ID:

Start Date:

Experiment Contact:

Developmental Stage CV:

Sex:

Organism:

Gene or target symbol:

Target type:

Primary target ID:

Fragment Producer Contact:

Fragment Label ID:

Fragment Batch ID:

Fragment concentration:

Fragment ID:

Fragment length:

Sense sequence:

Fragment Producer:

Efficacy of Knock Down:

Phenotype CV:

Phenotype:

Number of Individuals (End):

Gallery Image:

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