beta FTZ-F1

Submitted by Joakim Brunet on 14 June, 2018 - 13:50

Phenotype CV:

General

Experiment Batch ID:

Experiment bFTZ-F1

Experiment Contact:

Joakim Brunet

Start Date:

17 January, 2018 - 13:00

End Date:

24 January, 2018 - 13:00

Experiment Description:

Sampled larvae at middle/old nauplius 2 to measure downregulation before visible phenotype.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Developmental Stage CV:

Nauplius

Copepodid

Target description

Primary target ID:

EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis

EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

beta FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

468bp

Fragment concentration:

10ng/ul

Sense sequence:

ttgaggacaacaacgacgactcta Ctatactcattagctatacattgttgtgtggatttcgaaaagagacgcggacgataagga Gcagggaatttgtgatttcgaaaaaaaaatagtaaaaatgtgccatggtggtttgagatg Accaaatacatttataagggcaagttacgtgaaaaaggcccttgaatgttacccacggaa Cctcttattagtattccgtattctaaccaacgtggacgtttccatcaaccccatattcaa Gagctgcctctcgcagccccgggttctaatcatagatcatcatccctgatccttataaaa Aaagagccgaatatgagcacaagtggaagcttgagtagcggtggtagtcctcccatggtt Ggaggaacactcctcccaaccatggatatgccgatcggaaactcatttgcttccttggaa Gacctgagcagtgtagtccatcca

Fragment ID:

beta FTZ-F1, beta FTZ-F1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

beta FTZ-F1

Fragment Label ID:

beta FTZ-F1

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

Phenotype:

Developmental arrest

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Image file:

bFTZF1_31.5X_2018_01_24_9_6.JPG

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Soaking of larvae with dsRNA specific for the transcript variant beta for the gene FTZ-F1. Resulted in developmental arrest.

LsCHS1

Submitted by Hulda Maria Har... on 7 May, 2018 - 23:13

Phenotype CV:

General

Experiment Batch ID:

PAM1

Experiment Contact:

Hulda Maria Hardardottir

Start Date:

20 February, 2018 - 09:00

End Date:

26 February, 2018 - 10:11

Sample

Organism:

Lepeophtheirus salmonis

Sex:

males

Number of Individuals (Start):

Developmental Stage CV:

PreAdultI males

Target description

Primary target ID:

EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment length:

174bp

Fragment concentration:

498ng/ul

Sense sequence:

TGGTGTGAGGCGTTAGAACCATTGGAAAATAGTGCCGACATGCATCGGGATGCTCTGTACGGGCGTACGGGAGCATCAGACCTATACTCTAAAATGTCTCGCTCTGGGATGCAGGACTTGAGAGGAGGCGATGATCTTGATGAATTTACGGATGATGAAGCCACTCCACTCACG

Fragment Label ID:

Fragment Producer Contact:

Hulda Maria Hardardottir

Validation

Efficacy of Knock Down:

F>80%

Phenotype

Phenotype:

In preAdultII: Could not complete molting or had thin exoskeleton (could not swim).

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Primers name: ChS1_f_dsRNA_b4611, ChS1_r_dsRNA_b4612, the primers were designed before the transcripts were analyzed by sequencing and RACE.

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