beta FTZ-F1

Submitted by Joakim Brunet on 14 June, 2018 - 13:50

Phenotype CV:

General

Experiment Batch ID:

Experiment bFTZ-F1

Experiment Contact:

Joakim Brunet

Start Date:

17 January, 2018 - 13:00

End Date:

24 January, 2018 - 13:00

Experiment Description:

Sampled larvae at middle/old nauplius 2 to measure downregulation before visible phenotype.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Developmental Stage CV:

Nauplius

Copepodid

Target description

Primary target ID:

EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis

EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

beta FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

468bp

Fragment concentration:

10ng/ul

Sense sequence:

ttgaggacaacaacgacgactcta Ctatactcattagctatacattgttgtgtggatttcgaaaagagacgcggacgataagga Gcagggaatttgtgatttcgaaaaaaaaatagtaaaaatgtgccatggtggtttgagatg Accaaatacatttataagggcaagttacgtgaaaaaggcccttgaatgttacccacggaa Cctcttattagtattccgtattctaaccaacgtggacgtttccatcaaccccatattcaa Gagctgcctctcgcagccccgggttctaatcatagatcatcatccctgatccttataaaa Aaagagccgaatatgagcacaagtggaagcttgagtagcggtggtagtcctcccatggtt Ggaggaacactcctcccaaccatggatatgccgatcggaaactcatttgcttccttggaa Gacctgagcagtgtagtccatcca

Fragment ID:

beta FTZ-F1, beta FTZ-F1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

beta FTZ-F1

Fragment Label ID:

beta FTZ-F1

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

Phenotype:

Developmental arrest

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bFTZF1_31.5X_2018_01_24_9_6.JPG

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Soaking of larvae with dsRNA specific for the transcript variant beta for the gene FTZ-F1. Resulted in developmental arrest.

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Fragment length:

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Sense sequence:

Fragment ID:

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Efficacy of Knock Down:

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