RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort descending Publication
201 AC F231 EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
Adult, Adult 30/25 Lepeophtheirus salmonis F>99.50% 12 April, 2016 - 11:31
202 AF F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:33
203 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
204 AF F228 EMLSAG00000006953, EMLSAG00000006953-689719 (gene) Lepeophtheirus salmonis
6953
PreAdult2 30/15 Lepeophtheirus salmonis 16 July, 2016 - 11:45
205 AF F263 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis
441, 12943
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:51
206 AF F264 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000006733, EMLSAG00000006733-689499 (gene) Lepeophtheirus salmonis
441, 12943, 6733
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:56
207 AF F241 EMLSAG00000002443, EMLSAG00000002443-685209 (gene) Lepeophtheirus salmonis
T2_2443
PreAdult2, Adult no visible phenotype 30/21 Lepeophtheirus salmonis 16 July, 2016 - 12:00
208 Mucin-like spermatophore wall protein 2 (AG F270) EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis
MLSWP2
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>80.00% 26 January, 2017 - 14:55
A novel gene-family involved in spermatophore generation in...
209 Mucin-like spermatophore wall protein 1 (AG F269) EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis
MLSWP1
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>95.00% 26 January, 2017 - 15:07
A novel gene-family involved in spermatophore generation in...
210 Testis-specific 2489 AG F271 EMLSAT00000002489, EMLSAT00000002489-698336 (mRNA) Lepeophtheirus salmonis adult male no visible phenotype Lepeophtheirus salmonis 26 January, 2017 - 15:15

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