RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
191 F0 cop + infection CPY185 (negative control), CPY185 (PCR_product) Gadus morhua Nauplius1, Copepodid No visible phenotype 60/52 Lepeophtheirus salmonis 2 May, 2019 - 11:21
192 AT F393 + F149 EMLSAT00000004598, EMLSAT00000004598-700445 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/21 Lepeophtheirus salmonis F>70.00% 2 May, 2019 - 11:01
193 AT F394 + F149 EMLSAT00000005702, EMLSAT00000005702-701549 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/13 Lepeophtheirus salmonis F>69.00% 2 May, 2019 - 11:41
194 S F180 EMLSAT00000009473, EMLSAT00000009473-705320 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype but significant reduction in 30/24 Lepeophtheirus salmonis 26 February, 2015 - 14:41
195 RNAi_V 3422 EMLSAG00000003422, EMLSAG00000003422-686188 (gene) Lepeophtheirus salmonis PreAdult offspring deformations 120/41 Lepeophtheirus salmonis 14 January, 2015 - 08:21
196 L F97 EMLSAG00000002641, EMLSAG00000002641-685407 (gene) Lepeophtheirus salmonis PreAdult, Adult One animal for sectioning
female reproduction
29/7 Lepeophtheirus salmonis 7 May, 2013 - 10:13
197 L F98 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
Kiran
PreAdult, Adult One animal for sectioning
female reproduction
30 Lepeophtheirus salmonis 22 January, 2014 - 10:44
198 L F102 EMLSAT00000004662, EMLSAT00000004662-700509 (mRNA) Lepeophtheirus salmonis
UN21_g1627
PreAdult, Adult One animal sectioned
female reproduction, survival
29/4 Lepeophtheirus salmonis F>90.00% 6 June, 2013 - 10:00
199 F159 EMLSAG00000001069, EMLSAG00000001069-683835 (gene) Lepeophtheirus salmonis
Peroxidase
PreAdult1 female, PreAdult2 male Reduced survival 90/11 Lepeophtheirus salmonis F>90.00% 11 June, 2014 - 11:16
200 P F149 EMLSAG00000010881, EMLSAG00000010881-693647 (gene) Lepeophtheirus salmonis PreAdult2, Adult Reduced survival 39 Lepeophtheirus salmonis 11 June, 2014 - 13:50

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