RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
131 AO F359 EMLSAT00000010263, EMLSAT00000010263-706110 (mRNA) Lepeophtheirus salmonis
EMLSAT00000010263
PreAdult2 30 Lepeophtheirus salmonis F>60.00% 26 March, 2018 - 11:06
132 AP F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_1
PreAdult2, Adult no visible phenotype 30/20 Lepeophtheirus salmonis F>80.00% 27 March, 2018 - 13:36
133 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 27 March, 2018 - 13:43
134 AP F246 EMLSAT00000008875, EMLSAT00000008875-704722 (mRNA) Lepeophtheirus salmonis
T9_8875
PreAdult2, Adult no visible phenotype 30/16 Lepeophtheirus salmonis 27 March, 2018 - 13:54
135 AP F247 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T11_5706
PreAdult2, Adult no visible phenotype 31/20 Lepeophtheirus salmonis 27 March, 2018 - 13:57
136 AP F245 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T10_8281
PreAdult2, Adult no visible phenotype 31/24 Lepeophtheirus salmonis 27 March, 2018 - 14:00
137 AP F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/17 Lepeophtheirus salmonis 27 March, 2018 - 14:02
138 1316/11944/699 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis
testis genes
PreAdult 1 60 Lepeophtheirus salmonis 28 March, 2018 - 10:38
139 10162 EMLSAT00000010162, EMLSAT00000010162-706009 (mRNA) Lepeophtheirus salmonis
10162
PreAdult 1 60 Lepeophtheirus salmonis 28 March, 2018 - 10:40
140 Rhabdovirus - No9+127 Specificity LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9, LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127
N protein LsRV-No9, N protein LsRV-No127
Nauplius I, Copepodid 540/540 Lepeophtheirus salmonis F>94.00% 19 April, 2018 - 15:00
RNAi-mediated treatment of two vertically transmitted...

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