AV F404

Submitted by Andreas Borchel on 2 May, 2019 - 09:49

Phenotype CV:

General

Experiment Batch ID:

AV

Experiment Contact:

Andreas Borchel

Start Date:

14 March, 2019 - 08:35

End Date:

24 April, 2019 - 08:23

Experiment Description:

injection into pradult females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult1

Adult

Target description

Primary target ID:

EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment length:

400bp

Fragment concentration:

600ng/ul

Sense sequence:

TGTTGGCCCTATGAGTCCATCT TTTTTGTGACTTTGATGCCTTGA

Antisense sequence:

TGATCCTTGGAAGAAAAGAGGATG TTCATCATTTCCAGCATTGAGTTT

Fragment Label ID:

F404

Fragment Mixture Formula:

50% of both, in total 600 ng/ul

Fragment Producer:

licelab

Fragment Producer Contact:

Andreas Borchel

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

15

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Knockdown of a testis-specific ion-pump in pre-adult1 males, checking reproduction in adults. Many males were lost, and no kncokdown observed.

Z F198

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:31

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000527, EMLSAG00000000527-683293 (gene) Lepeophtheirus salmonis

Gene or target symbol:

527

Fragment description

Fragment length:

415bp

Fragment concentration:

569ng/ul

Sense sequence:

cctcaggccccaaagctctggttattaaacttatttttgaataccaaggagagcgttctaatagtccaaaaataagaaagcttgtgctagaagaaggagagacgccgactctgggcttcttatgtgaatctttttctttaaaaagctcgcaaacattttattactacgatgatgacggggatcaaataactatatccaatgatcgagagcttctgacagccatcaaggaaaagaagtcctctgatacactaaggctctatacaggaacagagcttattccaggagatattccgagtaaaaggaaccatgagaaagatatttttgaagagatttgtaccaattgtcagaaccgaatcggtcgattctcttataaatgtgttatttgtagcggtgtgaagccttcggagtgcactcg

Fragment Label ID:

F198

Primer Group:

b3154

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

20

Phenotype:

slightly wavy ES-check hatching

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Unknown function of gene

Slightly wavy egg strings.

Downregulation not verified by qPCR.

Z F204

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:12

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000106, EMLSAG00000000106-682872 (gene) Lepeophtheirus salmonis

Gene or target symbol:

106

Fragment description

Fragment length:

553bp

Fragment concentration:

324ng/ul

Sense sequence:

actcccagctgcagctgtgatatagtttcttctcatattaataaacaaggaactagtgctatcatccctactttatcctgtagcaatgaacgctttaaaatatcggatctctctcgcagaaatactagagagtactcttcttctcctcaatgtgatcaaggactcattcttttaaaacgtcttcgaatagcagataaagagctcaaagcccatgaatggatgagtgtgtgtgggaatcataaaaaatatttgcttcaggattttcctctcaaaaaccaccaatgcggtatttgttttaacagtgatcaaatacggcttagaagagtttctacaaagtcgcaggatttgtacaaaggagaagtcgtggttggggaattcgtatgttcggagtgtgatgatttgatattagatggagaaggggatgagagtcagcataaaaaaataaccattgggcagatttgggttacattggataattcattttgtcttattaatagctctgtaccttcgctcagattattatgtagaggcttaaccaacaaagagccccttc

Fragment Label ID:

F204

Primer Group:

b3242

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

20

Phenotype:

Slightly wavy ES-check hatching

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Unknown function of gene.

Slightly wavy egg strings.

Downregulation not verified by qPCR.

P F120

Submitted by Christiane Trösse on 11 March, 2014 - 16:23

Phenotype CV:

General

Experiment Batch ID:

P

Experiment Contact:

Frank Nilsen

Start Date:

7 March, 2014 - 15:55

End Date:

22 April, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000693, EMLSAG00000000693-683459 (gene) Lepeophtheirus salmonis

Gene or target symbol:

UN28_g13803

Fragment description

Fragment length:

510bp

Fragment concentration:

600ng/ul

Sense sequence:

tgctctggaagatgatgaacacgatattacttttgctccgcccaattctccgattgtaccttccaatgttgttatgagaggcactatcaacattccttctcttgatcagactttgaatgcagaagacccagaaggagcatcccccattgttagaggagtttttgattcaacttggtttccatattccaactacgagaattgtccagttacccctgacgcaaaccaaacaacgtatggaagtgtatatccacacttatcttaccggcatgatctcacgtttcgagatcttcaacttgcttcctcttttcctaataatagcaaccaatttctgaatgaaagtaatatggaaattctacacaattctcttctagaatctaaatggaaacccaaggacaaaatatttgtggacctcaacaatacacttgatgaaaaagataatatcgactataactcaactattcttgcgaacaataataataacaacagtagtgtccttgagcccatgttcca

Fragment Label ID:

F120

Primer Group:

UN28_g13803_f_b1875

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>5%

Phenotype

Number of Individuals (End):

13

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3 individuals had very special curly eggstrings , 3 had no eggstrings , low reproduction

E F34

Submitted by Christiane Trösse on 12 March, 2013 - 09:05

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Sussie Dalvin

Start Date:

15 October, 2012 - 00:04

End Date:

26 November, 2012 - 00:00

Experiment Description:

<p>24 females recovered (62%), 7 with normal looking eggstrings, that hatch. Animals with eggstrings have blood in the gut, animals w/o eggstrings have little or no blood. Theis group of animals looked almost normal and was reproducing until 3 days before termination (last check). Seem to have been caught just when the system broke down. Reproducing animals had normally developed cops (morph and numbers)</p>

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000003242, EMLSAG00000003242-686008 (gene) Lepeophtheirus salmonis

Gene or target symbol:

g7499_LsRaptor

Fragment description

Fragment length:

451bp

Fragment concentration:

600ng/ul

Sense sequence:

tcaagattcctcctcaactgccgctactccttggagactccttgaacgaatgaaaactgtgagtgttgctcttgtggtatgcctcaatgtcggggtggatcctccggatgtgacgaaaacctccccttgtgcttgtattgaggcatggattgatccaactcttctcaatccaacaagagcacttaatctcattggatctgctcttctaagacaatatgaacgttggcaacccagggctaggtatagacaatctttggacccaactgtggacgaagtccgacgcctagctatggctctacgtaaaaacgctagggatgaaagagttctttttcattataatggtcatggggtcccgaaaccaacggcaaacggagagatttgggtttttaatcggaattacactcagtatattcccctgagtctctacgatcttcaaacatggatgggctcc

Fragment Label ID:

F34

Primer Group:

Raptor_f_b918

Fragment Producer:

Heidi

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

24

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Adult females recovered at termination of RNAi experiment. Experiment E.