C F4 (CopB2)

Submitted by Christiane Trösse on 24 July, 2015 - 16:11

Phenotype CV:

General

Experiment Batch ID:

C

Experiment Contact:

Christiane Trösse

Start Date:

14 August, 2012 - 09:00

End Date:

24 September, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>82%

Phenotype

Number of Individuals (End):

0

Upload

Image file:

4a.JPG

4b.JPG

Gallery Image:

Image Quality:

No votes yet

Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F4 (CopB2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:44

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>65%

Phenotype

Number of Individuals (End):

5

Upload

Image file:

9b.JPG

9c.JPG

Gallery Image:

Image Quality:

No votes yet

Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

E F42

Submitted by Heidi Kongshaug on 12 March, 2013 - 10:38

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Frank Nilsen

Start Date:

15 October, 2012 - 10:19

End Date:

26 November, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g13677_Rheb

Fragment description

Fragment length:

460bp

Fragment concentration:

600ng/ul

Sense sequence:

atggggcctcccaagtctcgtaaaattgccttaatgggagctgtttccgtgggcaaatcttctctggccatccaatttgtacaaggacaatttgtcgattcctacgaaccaacaatcgaaaatacatttaccaaaacagttaaaattaatagacaagaatatgaaatttgtcttgttgatacagcaggccaaaatgaatactctatctttccattggaatattctgtaggaatagatggatatgttcttgtatactccattgattcacctaaaagcttagaagtttgtcaaatcatacaggaaaaattaatagaattcacaggaaacccaaatgttcccattgtcttggttggcaataaaaatgatttgtatatggaaagatatgtttcctttgaagaaggcaaaagaagagctgaagaaatgaaagcagtgtttcttgagacctctgccaaggaaaacc

Fragment Label ID:

F42

Primer Group:

Rheb_f_b1063

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>60%

Phenotype

Number of Individuals (End):

25

Phenotype:

Low reproduction and most are missing blood in the gut

Upload

Image file:

img_0128.jpg

img_0129.jpg

img_0131.jpg

Gallery Image:

Image Quality:

No votes yet

Eggestrenger:

12.11 F42 ser ut som der ikke er noen sunne eggstrenge mer (har vært tidligere).

15.11 Lite blod i F42.

Phenotype CV:

Experiment Batch ID:

Experiment Contact:

Start Date:

End Date:

Organism:

Sex:

Number of Individuals (Start):

Developmental Stage CV:

Primary target ID:

Gene or target symbol:

Fragment length:

Fragment concentration:

Sense sequence:

Fragment Label ID:

Primer Group:

Fragment Producer:

Fragment Producer Contact:

Efficacy of Knock Down:

Number of Individuals (End):

Image file:

Gallery Image:

Image Quality:

Phenotype CV:

Experiment Batch ID:

Experiment Contact:

Start Date:

End Date:

Organism:

Sex:

Number of Individuals (Start):

Developmental Stage CV:

Primary target ID:

Gene or target symbol:

Fragment length:

Fragment concentration:

Sense sequence:

Fragment Label ID:

Primer Group:

Fragment Producer:

Fragment Producer Contact:

Efficacy of Knock Down:

Number of Individuals (End):

Image file:

Gallery Image:

Image Quality:

Phenotype CV:

Experiment Batch ID:

Experiment Contact:

Start Date:

End Date:

Organism:

Sex:

Number of Individuals (Start):

Developmental Stage CV:

Primary target ID:

Gene or target symbol:

Fragment length:

Fragment concentration:

Sense sequence:

Fragment Label ID:

Primer Group:

Fragment Producer:

Fragment Producer Contact:

Efficacy of Knock Down:

Number of Individuals (End):

Phenotype:

Image file:

Gallery Image:

Image Quality:

Search form