AS F379

Submitted by Andreas Borchel on 21 November, 2018 - 09:46

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000008518

Fragment description

Fragment length:

483bp

Fragment concentration:

600ng/ul

Sense sequence:

GGACGGTGTGTGACTCCAAACACCTGCAAGTGTGATTCTGGGTGGAGGGGTGATACCTGCAATGAGTGTATTCCCTCATCTCAGTGTCAAAATGGATTTTGCGTTCATTCTCCGAAGGAATGTATCTGCAGTTTGGGCTGGGCTGGAGTAAACTGTGATCTACAGGATCATGGATGTGTCCATGGAACCTTTTCATCAAAACATCACTGCACATGCGATCCGGGATGGTTAGGAAGAAATTGCAGTTCTTGTATACCGCACCCAAGTTGTGTTTGGGGATCATGTAAATCCACTCCGGGCGAATGTTTTTGTGATCCAGGATGGGATGGAAGGAATTGTGACATCCCCATTTGCGAGAAAGGGTGTCATCCAGATCACGGATTTTGTTTTAAGCCATCTCAATGTATATGTCGCTCTGGTTGGCATGGAAAACGTTGTGATCTTTGCACTCCAATGCCTGGATGCACACGAGGATGCCATAAA

Fragment Batch ID:

F379

Fragment Label ID:

F379

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

15

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knockdown lead to missing egg strings

X F201 (ZIP13)

Submitted by Christiane Trösse on 27 July, 2015 - 14:33

Phenotype CV:

General

Experiment Batch ID:

X

Experiment Contact:

Christiane Trösse

Start Date:

17 March, 2015 - 13:44

End Date:

22 April, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000012537, EMLSAG00000012537-695303 (gene) Lepeophtheirus salmonis

Gene or target symbol:

ZIP13

Fragment description

Fragment length:

546bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F201

Primer Group:

b3122

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Phenotype

Number of Individuals (End):

7

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RNAi with preadult females. 86% of recovered lice had missing or abnormal egg strings, but could be a coincidence (low lice numbers). No hatching data from IMR. qPCR validation not done yet. Fotos (RX28-32, RFX8-9) on P:\Sealice\RNAi\RNAi_fotos_samplet\Experiment_X\RNAiX).

U F52

Submitted by Heidi Kongshaug on 8 December, 2014 - 13:56

Phenotype CV:

General

Experiment Batch ID:

U

Experiment Contact:

Sussie Dalvin

Start Date:

3 December, 2014 - 13:54

End Date:

14 January, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAG00000010179, EMLSAG00000010179-692945 (gene) Lepeophtheirus salmonis

Gene or target symbol:

g4675

Fragment description

Fragment length:

494bp

Fragment concentration:

600ng/ul

Sense sequence:

cgagtgacacaacgccgcaagaagtccaaaccactacgactaaagcgatgtcttccacggagcaagaacctacaacgacgactctactttctgactccaaagaatcatctgagcccaaaaaagaactcacaaagaaaatgagcataaaaaaggaccaagaacatacaattgattcaataattccatgtactgatatagatgaggacgcttgtcaatcttttaaaccactttgtaaaatcgaaatcgaaataggatttcgtcgttgtcaaaaaacatgcggtttttgcaaggggaagagacttatttatgaggccaaaatttcaaacggtgttgaaggacttgtagaatttcaacaattttatccgagacagcctgtatatatggctggttgttttgaaaaatctggtataaaaaacacaattactccacacgcttttgttattcatgaaagaagtgaagaaccttcaatgaaggattccaaatgcccacatgca

Fragment Label ID:

F52

Primer Group:

g4675_f_b1230

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

10

Phenotype:

Non-hatching eggstrings

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This experiment was performed by injection of males only in the normal set-up, i.e. young adult males and placed on fish together with non-injected females. Only males were sampled at termination, but females were photographed and eggstrings put for hatching (photos named as the hatching well).

T F166

Submitted by Heidi Kongshaug on 14 November, 2014 - 13:26

Phenotype CV:

General

Experiment Batch ID:

T

Experiment Contact:

Frank Nilsen

Start Date:

30 October, 2014 - 13:20

End Date:

9 December, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000010071, EMLSAT00000010071-705918 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

UN50_10071

Fragment description

Fragment length:

457bp

Fragment concentration:

600ng/ul

Sense sequence:

cgaatccgcagatgaaggagtctatgacttctgggtgtctccacgtgtgggctcttatgctaaggtaatggttgctcctggatacgtttctacattcgaacaattcttgaaggagcaayccattgcctctactcttttgattgaggatgtggaaattactaatcaaagattttccatcattgaaaatgctttaagaaaaatcaaaacttcatccagatcaattgactttgaaaactactacagttcagacgatcttraagccgataatggctttgtcgaagttgaaacctatggaaaatcctacgaaggtagagacttaaaggtcatcaagattatgaaggctggacctaatgctcccaatatcttcattgaggctggaattcatgcacaagatcctgaaaatgctcgttaccttgaccaattcaatttccacatcatgccttccgttaaccccgat

Fragment Label ID:

F166

Primer Group:

likb2496_T7_b2546

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>96%

Phenotype

Number of Individuals (End):

19

Phenotype:

Abnormal

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Short and malformed eggstrings, no mortality

R F161

Submitted by Heidi Kongshaug on 7 October, 2014 - 10:17

Phenotype CV:

General

Experiment Batch ID:

RNAi R

Experiment Contact:

Frank Nilsen

Start Date:

21 August, 2014 - 10:10

End Date:

1 October, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

37

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000580, EMLSAG00000000580-683346 (gene) Lepeophtheirus salmonis

Gene or target symbol:

UN38_580

Fragment description

Fragment length:

472bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F

Primer Group:

b2527

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

20

Phenotype:

Abnormal eggstrings in some individuals

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The experiment was performed to check the phenotype of the gene. Abnormal eggstringproduction was observed at termination. Normal looking copepods developed from the eggstrings. Normal Activity. Sample RFR2 was sent for sectioning, abnormal