RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
321 AV F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_1
PreAdult2 normal
no visible phenotype
30/11 Lepeophtheirus salmonis 3 May, 2019 - 13:15
322 AV F350 EMLSAG00000009839, EMLSAG00000009839-692605 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/20 Lepeophtheirus salmonis 2 May, 2019 - 11:17
323 AV F0 extra CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal
no visible phenotype
30/17 Lepeophtheirus salmonis 2 May, 2019 - 09:59
324 AV F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal
no visible phenotype
30/13 Lepeophtheirus salmonis 2 May, 2019 - 10:16
325 AU F336 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis Adult no visible phenotype 30/6 Lepeophtheirus salmonis 2 May, 2019 - 10:16
326 AU F0 male CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult No visible phenotype
Control
30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:56
327 AU F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult No visible phenotype
Control
30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:37
328 AT F394 + F149 EMLSAT00000005702, EMLSAT00000005702-701549 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/13 Lepeophtheirus salmonis F>69.00% 2 May, 2019 - 11:41
329 AT F393 + F149 EMLSAT00000004598, EMLSAT00000004598-700445 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/21 Lepeophtheirus salmonis F>70.00% 2 May, 2019 - 11:01
330 AS F384 EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis PreAdult2 reproduction, female reproduction, no eggstrings 30/21 Lepeophtheirus salmonis F>99.00% 21 November, 2018 - 11:10

Pages