RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
201 M-F113 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype 40/13 Lepeophtheirus salmonis 14 March, 2017 - 17:44
202 M-F112 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype 40/17 Lepeophtheirus salmonis 14 March, 2017 - 17:36
203 M F107 EMLSAG00000006881, EMLSAG00000006881-689647 (gene) Lepeophtheirus salmonis
UN26_g4260
PreAdult2, Adult No visible phenotype
no visible phenotype
40/10 Lepeophtheirus salmonis 6 June, 2013 - 10:05
204 M F0 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis PreAdult, Adult Control 37/6 Lepeophtheirus salmonis 12 June, 2013 - 12:17
205 LsRXR EMLSAT00000001919, EMLSAT00000001919-697766 (mRNA) Lepeophtheirus salmonis
LsRXR
Adult, Copepodid female reproduction, reduced offspring viability, shorter eggstrings 50/38 Lepeophtheirus salmonis F>60.00% 11 April, 2016 - 12:18
Characterization of a novel RXR receptor in the salmon louse...
206 LsHSCARB on free living stages EMLSAT00000005382, EMLSAT00000005382-701229 (mRNA) Lepeophtheirus salmonis, EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis Nauplius I, Copepodid none
no visible phenotype
300/250 Lepeophtheirus salmonis F>99.00% 23 April, 2018 - 10:06
A scavenger receptor B (CD36)-like protein is a potential...
207 LsHSCARB - a putative intestinal heme receptor EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis PreAdult2 Shorter egg-strings, 60% less heme, lower hatching
feeding, reduced nutrient uptake
30/15 Lepeophtheirus salmonis F>95.00% 4 December, 2017 - 10:55
A scavenger receptor B (CD36)-like protein is a potential...
208 LsCHS2 EMLSAT00000007308, EMLSAT00000007308-703155 (mRNA) Lepeophtheirus salmonis
LsCHS2
PreAdult1 , Adult None 9 Lepeophtheirus salmonis F>60.00% 14 June, 2018 - 11:54
209 LsCHS2 EMLSAT00000007308, EMLSAT00000007308-703155 (mRNA) Lepeophtheirus salmonis
LsCHS2
PreAdult2, Adult None 60/12 Lepeophtheirus salmonis 14 June, 2018 - 13:35
210 LsCHS1 EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis
LsCHS1
PreAdult2 None 60/4 Lepeophtheirus salmonis 14 June, 2018 - 13:05

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