RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending Publication
151 AI F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:38
152 AH F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/21 Lepeophtheirus salmonis 22 March, 2018 - 14:36
153 AH F243 EMLSAG00000005306, EMLSAG00000005306-688072 (gene) Lepeophtheirus salmonis
T4_5306
PreAdult2, Adult No visible phenotype
no visible phenotype
30/17 Lepeophtheirus salmonis 22 March, 2018 - 14:30
154 LsHSCARB - a putative intestinal heme receptor EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis PreAdult2 Shorter egg-strings, 60% less heme, lower hatching
feeding, reduced nutrient uptake
30/15 Lepeophtheirus salmonis F>95.00% 4 December, 2017 - 10:55
A scavenger receptor B (CD36)-like protein is a potential...
155 AG-F286 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011448, EMLSAT00000011448-707295 (mRNA) Lepeophtheirus salmonis PreAdult2 Slightly curly eggstrings. 29/19 Lepeophtheirus salmonis 16 March, 2017 - 11:15
156 X F210 EMLSAT00000011448, EMLSAT00000011448-707295 (mRNA) Lepeophtheirus salmonis PreAdult2 Eggstrings curly and strange. 30/5 Lepeophtheirus salmonis 14 March, 2017 - 22:02
157 X F209 EMLSAT00000011090, EMLSAT00000011090-706937 (mRNA) Lepeophtheirus salmonis PreAdult2 Short egg-strings. 30/11 Lepeophtheirus salmonis 14 March, 2017 - 21:36
158 M-F115 EMLSAT00000008639, EMLSAT00000008639-704486 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype 40/11 Lepeophtheirus salmonis 14 March, 2017 - 18:04
159 M-F114 EMLSAT00000001502, EMLSAT00000001502-697349 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype 40/13 Lepeophtheirus salmonis 14 March, 2017 - 17:52
160 M-F113 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype 40/13 Lepeophtheirus salmonis 14 March, 2017 - 17:44

Pages