RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
181 AC F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
Adult female reproduction, no eggstrings 31/21 Lepeophtheirus salmonis 31 March, 2016 - 09:56
182 AC F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult Control 30/28 Lepeophtheirus salmonis 22 March, 2016 - 09:10
183 RNAi_AE 9341 EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis preadult I male, preadult I and II female 95 Lepeophtheirus salmonis 23 February, 2016 - 10:18
184 RNAi_AE 3422 EMLSAG00000003422, EMLSAG00000003422-686188 (gene) Lepeophtheirus salmonis preadult II male, preadult I and II female 78 Lepeophtheirus salmonis 23 February, 2016 - 10:15
185 RNAi_AE 5667 EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis preadult I male, preadult I and II female 77 Lepeophtheirus salmonis 23 February, 2016 - 10:12
186 RNA_AE F255 lcl|LsalIR338-Anna|LsalIR338, lcl|LsalIR338-Anna|LsalIR338.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR331-Anna|LsalIR331, lcl|LsalIR331-Anna|LsalIR331.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR324-Anna|LsalIR324, lcl|LsalIR324-Anna|LsalIR324.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR339-Anna|LsalIR339, lcl|LsalIR339-Anna|LsalIR339.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR340-Anna|LsalIR340, lcl|LsalIR340-Anna|LsalIR340.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR8a.2-Anna|LsalIR8a.2, lcl|LsalIR8a.2-Anna|LsalIR8a.2.mrna1 (mRNA) Lepeophtheirus salmonis preadult II male, preadult I and II female 96 Lepeophtheirus salmonis 23 February, 2016 - 10:09
187 RNAi_AE F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua preadult II male, preadult I and II female 96 Lepeophtheirus salmonis 23 February, 2016 - 10:01
188 NAK F226+F227 EMLSAT00000000441, EMLSAT00000000441-696288 (mRNA) Lepeophtheirus salmonis, EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 11:04
189 NAK F227 EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 11:01
190 NAK F226+F227+F205 EMLSAT00000000441, EMLSAT00000000441-696288 (mRNA) Lepeophtheirus salmonis, EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis, EMLSAT00000006733, EMLSAT00000006733-702580 (mRNA) Lepeophtheirus salmonis
LsPGES2, LsPGES3, LsPGES1
Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 10:58

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