RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending Publication
181 AL F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30 Lepeophtheirus salmonis 22 March, 2018 - 14:42
182 AJ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:41
183 AG F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/15 Lepeophtheirus salmonis 22 March, 2018 - 14:40
184 AI F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/12 Lepeophtheirus salmonis 22 March, 2018 - 14:38
185 AH F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/21 Lepeophtheirus salmonis 22 March, 2018 - 14:36
186 AH F243 EMLSAG00000005306, EMLSAG00000005306-688072 (gene) Lepeophtheirus salmonis
T4_5306
PreAdult2, Adult No visible phenotype
no visible phenotype
30/17 Lepeophtheirus salmonis 22 March, 2018 - 14:30
187 AH F242 EMLSAG00000001206, EMLSAG00000001206-683972 (gene) Lepeophtheirus salmonis
T3_1206
PreAdult2, Adult no visible phenotype 30/19 Lepeophtheirus salmonis 22 March, 2018 - 14:27
188 LsHSCARB - a putative intestinal heme receptor EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis PreAdult2 Shorter egg-strings, 60% less heme, lower hatching
feeding, reduced nutrient uptake
30/15 Lepeophtheirus salmonis F>95.00% 4 December, 2017 - 10:55
A scavenger receptor B (CD36)-like protein is a potential...
189 ExpC EMLSAT00000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid host related phenotype, modulation of immune response 420/50 Lepeophtheirus salmonis F>99.00% 4 December, 2017 - 10:32
190 EMLSAT00000006642_copepodites EMLSAT00000006642, EMLSAT00000006642-702489 (mRNA) Lepeophtheirus salmonis Copepodid 420/66 Lepeophtheirus salmonis F>86.00% 4 December, 2017 - 10:28

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