RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending Publication
171 AF F263 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis
441, 12943
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:51
172 AF F228 EMLSAG00000006953, EMLSAG00000006953-689719 (gene) Lepeophtheirus salmonis
6953
PreAdult2 30/15 Lepeophtheirus salmonis 16 July, 2016 - 11:45
173 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
174 AF F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:33
175 AF F239 (F233+F234) EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis, EMLSAT00000002881, EMLSAT00000002881-698728 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010949, EMLSAG00000002881
Adult, Adult 30/25 Lepeophtheirus salmonis 12 April, 2016 - 11:21
176 F235 EMLSAT00000003009, EMLSAT00000003009-698856 (mRNA) Lepeophtheirus salmonis
EMLSAG00000003009
Adult, Adult 30/24 Lepeophtheirus salmonis 12 April, 2016 - 11:07
177 AC F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/24 Lepeophtheirus salmonis F>99.91% 12 April, 2016 - 10:35
178 EMLSAG00000013035 EMLSAT00000013035, EMLSAT00000013035-708882 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/20 Lepeophtheirus salmonis 12 April, 2016 - 10:26
179 LsRXR EMLSAT00000001919, EMLSAT00000001919-697766 (mRNA) Lepeophtheirus salmonis
LsRXR
Adult, Copepodid female reproduction, reduced offspring viability, shorter eggstrings 50/38 Lepeophtheirus salmonis F>60.00% 11 April, 2016 - 12:18
Characterization of a novel RXR receptor in the salmon louse...
180 AC F230 EMLSAG00000000504, EMLSAG00000000504-683270 (gene) Lepeophtheirus salmonis
504
Adult 31/24 Lepeophtheirus salmonis 31 March, 2016 - 14:29

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