RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
411 AS F384 EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis PreAdult2 reproduction, female reproduction, no eggstrings 30/21 Lepeophtheirus salmonis F>99.00% 21 November, 2018 - 11:10
412 AS F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY
PreAdult2 30/14 Lepeophtheirus salmonis 21 November, 2018 - 11:14
413 AV F404 EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult reproduction, female reproduction, egg string deformation, no eggstrings 30/15 Lepeophtheirus salmonis F>50.00% 2 May, 2019 - 09:49
414 AU F336 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis Adult no visible phenotype 30/6 Lepeophtheirus salmonis 2 May, 2019 - 10:16
415 AX F418 EMLSAT00000010925, EMLSAT00000010925-706772 (mRNA) Lepeophtheirus salmonis
Trypsin- Dylan
PreAdult2 survival, reduced 30/3 Lepeophtheirus salmonis 5 August, 2019 - 13:49

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