no visible phenotype

NAH F135

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

NAH

Experiment Contact: 

Christiane Trösse

Start Date: 

21 November, 2014 - 00:00

End Date: 

12 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

300

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

EMLSAG00000009319, EMLSAG00000009319-692085 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

BDH2
Fragment description

Fragment length: 

602bp

Fragment concentration: 

10ng/ul

Fragment Label ID: 

F135

Primer Group: 

1854

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

34
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Image icon J4_BDH2.JPG

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis, and with elevated expression from the parasitic copepodid stage. Infection of fish with knockdown copepodids (3 fish with 80 lice) and lice count after 16 and 42 days. No significant difference in number of lice on fish at termination. qPCR validation not done yet.

NAH F0

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

NAH

Experiment Contact: 

Christiane Trösse

Start Date: 

21 November, 2014 - 00:00

End Date: 

12 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

300

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol: 

CPY 185
Fragment description

Fragment concentration: 

10ng/ul

Fragment Label ID: 

F0

Primer Group: 
Phenotype

Number of Individuals (End): 

20
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Image icon J2_CPY185.JPG

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis, and with elevated expression from the parasitic copepodid stage. Infection of fish with knockdown copepodids (3 fish with 80 lice) and lice count after 16 and 42 days. One fish died 18.12.2014 -> results missing. No significant difference in number of lice on fish at termination. qPCR validation not done yet.

S F186 (FLVCR2)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

S

Experiment Contact: 

Christiane Trösse

Start Date: 

14 October, 2014 - 11:05

End Date: 

14 November, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

Adult
Adult
Target description

Primary target ID: 

EMLSAG00000005336, EMLSAG00000005336-688102 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

FLVCR2
Fragment description

Fragment length: 

533bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F186

Primer Group: 

b2654

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

23
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RNAi with adult females. No visible phenotype. No hatching data from IMR. qPCR validation not done yet. Fotos (RS160-179, RFS28-30) at P:\Sealice\RNAi\RNAi_fotos_samplet\Experiment_S\RNAiS.

S F172 (FLVCR)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

S

Experiment Contact: 

Christiane Trösse

Start Date: 

14 October, 2014 - 11:05

End Date: 

14 November, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

Adult
Adult
Target description

Primary target ID: 

EMLSAG00000007480, EMLSAG00000007480-690246 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

FLVCR
Fragment description

Fragment length: 

513bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F172

Primer Group: 

b2352

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

24
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RNAi with adult females. No visible phenotype. No hatching data from IMR. qPCR validation not done yet. Fotos (RS18-38, RFS4-6) at P:\Sealice\RNAi\RNAi_fotos_samplet\Experiment_S\RNAiS.

R F171 (Fepo)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

R

Experiment Contact: 

Christiane Trösse

Start Date: 

21 August, 2014 - 10:23

End Date: 

30 September, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000007383, EMLSAG00000007383-690149 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

Fepo (Ferroportin)
Fragment description

Fragment length: 

661bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F171

Primer Group: 

b2335

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

22
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RNAi with preadult females. No visible phenotype. Hatching rate not determined yet, but looks no different from control. qPCR validation not done yet.

R F170 (DcytB)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

R

Experiment Contact: 

Christiane Trösse

Start Date: 

21 August, 2014 - 10:23

End Date: 

30 September, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000005522, EMLSAG00000005522-688288 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

DcytB
Fragment description

Fragment length: 

546bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F170

Primer Group: 

b2329

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

19
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RNAi with preadult females. No visible phenotype. Hatching rate not determined yet, but looks no different from control. qPCR validation not done yet.

M F119 (DMT-1)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

M

Experiment Contact: 

Christiane Trösse

Start Date: 

28 May, 2013 - 13:08

End Date: 

2 July, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000004199, EMLSAG00000004199-686965 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

DMT-1
Fragment description

Fragment length: 

550bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F119

Primer Group: 

b1802

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>45%
Phenotype

Number of Individuals (End): 

13
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Image file: 

Image icon 8a
Image icon 8b
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RNAi in preadult females gave no visible phenotype and no difference in offspring.

Y F202

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Y

Experiment Contact: 

Frank Nilsen

Start Date: 

6 May, 2015 - 12:21

End Date: 

8 June, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000000496, EMLSAG00000000496-683262 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

496
Fragment description

Fragment length: 

452bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cgtcgtagctggggctatggagttttactcggtcttacgatgataggaagtttaattttaaactcatcattcttgatgagtttttggacatggcccaatcaaaggaaacatgcgccctcaattgttttactcatgtttaccatccgagacttgttcgtgacgatatttttaattccaacagctatagcttggttagttttcggggaaggaaaatggatatccgggagtgagtggtgcacgatatctggctttttggattttttcctggctgcagaatacccttttcttattttatgtctatgcattgttttaatcacgaggaaactaactaaatactacgatgatttttttgatcctgcacttgatgaagaggatgatcatcgaaaagtggatgagtatattcaaagcataccaacattgtcagtacctggagtgactgtggttggagttgg

Fragment Label ID: 

F202

Primer Group: 

likb3226_T7_b3238

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>74%
Phenotype

Number of Individuals (End): 

11
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Image file: 

File RY53.tif

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Downregulation of 496.

Y F200

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Y

Experiment Contact: 

Frank Nilsen

Start Date: 

6 May, 2015 - 12:21

End Date: 

8 June, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000000562, EMLSAG00000000562-683328 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

562
Fragment description

Fragment length: 

444bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cctgtcccaactttgagcacttgaattttcaacccaaaaattttgcatcaagacaatgcaattgggatggaaaatggtacgacaatcttacatccaaggcctttacacgctacgactcatgcccagactcggagagctttttgtataatgacattataaataaactctatgtcacgggatactccatttccctaggatttctcctttttgcaacagccatttttctattcttcagatcccttcgctgtattcgtaacagtatccatactcacctctttatcgcttccatattccataacctatgttggatcatttggtaccaaaccgtcatttatcaaatgggctctgtcatctcctggtcggaccaccattttctcttttgcaaaatcctccacgttcttacgaattattttatggtctgcacatacatgtggatgtttggtg

Fragment Label ID: 

F200

Primer Group: 

likb3248_T7_b3252

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>21%
Phenotype

Number of Individuals (End): 

15
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Image file: 

File RY23.tif

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Downregulation of 562

X F199

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

X

Experiment Contact: 

Heidi Kongshaug

Start Date: 

17 March, 2015 - 10:01

End Date: 

22 April, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000000147, EMLSAG00000000147-682913 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

700bp

Fragment concentration: 

496ng/ul

Sense sequence: 

tgggtgcagtttatggctctcaaaactggcacgccctaaggacaacctttagtcttcttcccttccatggattctggagtcaacctaggacagagcgtcaagccagatccaaaggatatatcaagatcagcgactgcaatgatatcaatgcgttccctgggagtcgttacattccaaactatgatcaccccaacattgttttcatttatgatgtaaatggatatattgctggaattcaaagtattgtggatcaggtaagaggaatatcaaatgatttatcatcacagccctactaatatgtatgtcgtcctgatttaaccaataaaaaaactcaggatcagggaaaaatcccaacttatttttttaaaatcatgtagatgttacgaggaggctggaaaatcggccattttgcaaaatgcccgggcaatctgtacaatttccaatagagtggtcaatgtttattttaaattcctttacaatgtcatttaaaatgattctgagtaatattattactaaacaattcattgtaagcaatttgatcattatggatttaattaacatacaatttcattgaacttaaagtagaatttctaattaaaattgttgcaaaatccaagcgcatagagcgctcataatacgcacaaagattgaaatatatttgagagtacctcaatttttctcggactctcaaaaggatggc

Fragment Label ID: 

F199

Primer Group: 

likbb3156_b3158

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

7

Phenotype: 

No visible phenotype
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Image file: 

File RX75.tiff

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Downregulation of EMLSAT00000000147

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