no visible phenotype

S F163

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

S

Experiment Contact: 

Frank Nilsen

Start Date: 

14 October, 2014 - 11:18

End Date: 

14 November, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

31

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

EMLSAT00000010954, EMLSAT00000010954-706801 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN46_10954
Fragment description

Fragment length: 

506bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atgtggcccaaactggcttgtttaacacttttatttgcgcttgcaaactgcaactacaatggatacaaagtattacaaaccgaagatattcattcccaagcaaaaaccattgaattgatcaaattcatcgaatccgcagatgaaggaatctatgacttctgggtctctccacgagttggctcttatgctaaagtgatggttgctccaggacacgtttctgaattcgaaaaattcttgaaagagcaatccattgcctctactcttttgattgaggatgtggaaattaccaatcaaagagcttccaccgttcaaaatgttttaagaaaactcaagacttcatccaggtccattgattttgaaaactactacagttcagatgacatctttgcttatttgcatgagcttgaagctgataacgactttgtcgaagtagaaacctatggnaaatcctacgaaggtagagacttaaaggtcatcaagattctgaaggctggacctaatgctcc

Fragment Label ID: 

F163

Primer Group: 

likb2488_T7_b2531

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>5%
Phenotype

Number of Individuals (End): 

21

Phenotype: 

Some abnormal
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Some animals lokked normal whereas other were definitely not normal

S F164

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

s

Experiment Contact: 

Frank Nilsen

Start Date: 

14 October, 2014 - 11:12

End Date: 

14 November, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

EMLSAT00000009656, EMLSAT00000009656-705503 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN47_9656
Fragment description

Fragment length: 

476bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcagcgactgcagtgacggagaatcgttccctgggaatcgttacattccaaattacgatcaccccaacattgttctcatttatgacgttaatggatatattgctgggattcaaagtattgtagatcaggatgcctgggacaatggctakgtaaacttccacgagaaatcaatgtatcagccaagtactattaatggaaaagaggttttcctaaccacggcttatttcgtggacccacaaattatttgctcaactggaagaactcgttatcaatacaaaacccaaggaacaggatatacacttattttccaaaatggagaagacatttcccaaaagaacttgatggaaatccctctaatagaagaagatttgaaggaatctgatttttggaacgaacatctttgtttcatcaacatgggcaagcattattttaatcttcattatgatcctgaggagtcctgtggaaatcttttccc

Fragment Label ID: 

F164

Primer Group: 

likb2490_T7_b2542

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

22

Phenotype: 

Normal
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Image file: 

File RS95.tif

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Downregulation of unknown 47

S F162

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

S

Experiment Contact: 

Frank Nilsen

Start Date: 

14 October, 2014 - 11:05

End Date: 

14 November, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

EMLSAT00000008265, EMLSAT00000008265-704112 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN41_3352
Fragment description

Fragment length: 

387bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ttcttggctatcattgcatctgctttggctaatccaaaatatccagatacttgtggtttggaaaacaaacctttagcggattcaaaaattgttggaggacaggaagcagcaaggaatcagtttccatggctagcttctgtatatacctttggtgcttgcactggttctgttttggatgaaaattggattataactgctaagcattgtataatacctcttgagggtgccaaagttcgtgttggcgttcatagtattgcagatggtgcatatgaaccaaatatgcaaacccgaaacagttctgaaattcacgtatcgtcgactggagactttgctttaatcaaattggagagtcctttggaattaaatgaatatgtccgacccgtttgc

Fragment Label ID: 

F162

Primer Group: 

likb2478_T7_b2529

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

21

Phenotype: 

Normal
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File RS84.tif

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Downregulation of unknown 41

R F124

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RNAi R

Experiment Contact: 

Frank Nilsen

Start Date: 

21 August, 2014 - 10:23

End Date: 

1 October, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAG00000012828, EMLSAG00000012828-695594 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN32_g22197
Fragment description

Fragment length: 

558bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F124

Primer Group: 

likb1883_T7_b1915

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

24

Phenotype: 

Normal
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Image file: 

Image icon IMG_0060_15A_5.JPG

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Downregulation of gene with unknown function

O F89

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

O

Experiment Contact: 

Sussie Dalvin

Start Date: 

2 December, 2013 - 10:45

End Date: 

8 January, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000004810, EMLSAG00000004810-687576 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN6
Fragment description

Fragment length: 

494bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tccaaaaggatccccttcttgaatgtactcacaagaatgtcgaaaagtgtcactatacctacatcacacaattttctccttctcaagaggaagtatgcgatgaaaattttgaaaaaagctgtcaaatcaccttcaaacaacaagcctttaatgaaaccatcaagaaatgctacaagccccttgaaaaggtatgtaatggacaaggtccagaagagtgtcgtacactctatgaatcctcctgtaccaccaagtacgttgagaagcaacctggtaagtttgttggtgatacaagctgtgaaaagctaccaatcgagatctgtggggctggatgtactactgaagaaggtcctgaagaatgtcacgacaaaactgttacttctcttgttgatatcccagaagaaatctgtgatttgaacccccaaaagacctgtagattccaaacaaagttagtccctagacttaagcctgcccacgaatgtaccacaattcctcaa

Fragment Label ID: 

F89

Primer Group: 

UN6_g394_f_b1396

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
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File ro113.tiff

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Knockdown of gene with unknown function

O F88

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

O

Experiment Contact: 

Sussie Dalvin

Start Date: 

2 December, 2013 - 10:45

End Date: 

8 January, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

38

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000012670, EMLSAG00000012670-695436 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN16
Fragment description

Fragment length: 

403bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gacgaatgcgaaggctcctacgtatcctcatcagtcctcacagtagggcccatctatgaagacatagttgaaaataaaacacccgaagtcctctactacaactctccaatacaaccttcttctgagcaaactcctcccccacctttaccctcaggaccccgaggacgctcaaatatgaatattagtaatgcttctcctaatttaccaccagattccgctggcccctcctcgcattgtaccaccagttccagcgaagttagcatcgattccaattcctcacccatcaaacatgtcgttctcaacccaactagcggagcacatcatcacccccatcaccatcctagtagacccttttcaacccctcgcactccacctgctcgcacaggtggagtttattactaca

Fragment Batch ID: 

O

Fragment Label ID: 

F88

Primer Group: 

UN5_g339_f_b1394

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

10
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File ro101.tiff

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Knockdown of gene with unknown function.

P F108

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

P

Experiment Contact: 

Frank Nilsen

Start Date: 

7 March, 2014 - 15:55

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000003438, EMLSAG00000003438-686204 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN27_g18393
Fragment description

Fragment length: 

438bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gtcaacaagccatccgagccaaaatccttcgaaataaggacaaccgattgggccatgtgctgggaaatgtttctcgagacgaaatcaatactggaactggaaacgaggaacttgaacctgtgaggatgagggcaactccattacggagaatacaggacgaagcggtgactcctgtgcctctctccactcctcctccttcaatctttaatttatccgggaacaaccaggtggctcctggggttgaagctccaactgtaaatgagagactcatctgctgtggctggctcctctccggactcttgactcgactgctcctccatttctttcccaatctcaatgccttcatcattttctctatcactgccttttctgttcaaacactacgcctcattgacttaagggcttcttccaattccaatgtatcccttcttcgtctcg

Fragment Label ID: 

F108

Primer Group: 

UN27_g18393f_b1697

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

11
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Image file: 

Image icon RP62.JPG

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    no obvious phenotype              no obvious phenotype              no obvious phenotype              no obvious phenotype              no obvious phenotype                                                                         

P F109

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

P

Experiment Contact: 

Frank Nilsen

Start Date: 

7 March, 2014 - 15:55

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000012670, EMLSAG00000012670-695436 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN16_g1269
Fragment description

Fragment length: 

470bp

Fragment concentration: 

600ng/ul

Sense sequence: 

agtgtcagagtccttgatggccgttcagataaagtgacttgtatgaaaagaattttactctccttgttcctccttctmctgacagccattttggctggaatcctctggattacactcattggtccaccaaattctgtatgtctagaagaaaaaggttttaacaatactcgatatacaaatccagatggaacctatcgggaacttagttacattttcattgaaaaagaacccaaaagacatttttatgcctttaaagagggaaaaagtaaatgtttggaactaggagccgaaatatgggaagtcgttggtgaagaagcagaatggaacttgttctacaacatagccacgaaacgaaacataattggacctagagggtctggaatatggatcaatgcaataatgaatcaaaaatgccctgagcagccctccaagaattgcgtcgaggaa

Fragment Label ID: 

F109

Primer Group: 

UN16_g1269_f_b1475

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>20%
Phenotype

Number of Individuals (End): 

12
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Image file: 

Image icon RP47.JPG

Gallery Image: 

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  Knockdown of gene with unknown function                                                                                                            

P F155 (HRG1)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

P

Experiment Contact: 

Christiane Trösse

Start Date: 

7 March, 2014 - 15:55

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000008285, EMLSAG00000008285-691051 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

HRG1
Fragment description

Fragment length: 

435bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F155

Primer Group: 

b2347
Validation

Efficacy of Knock Down: 

F>57%
Phenotype

Number of Individuals (End): 

12
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   RNAi with preadult females. No visible phenotype and no difference in offspring.                                                                                                                          

P F154 (HCP1)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

P

Experiment Contact: 

Christiane Trösse

Start Date: 

7 March, 2014 - 15:55

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000003292, EMLSAG00000003292-686058 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

HCP1
Fragment description

Fragment length: 

541bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F154

Primer Group: 

b2341
Validation

Efficacy of Knock Down: 

F>74%
Phenotype

Number of Individuals (End): 

8
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    RNAi with preadult females. No visible phenotype and no difference in offspring.                                                                                                                         

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