no visible phenotype

J F72

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Phenotype CV: 

General

Experiment Batch ID: 

J F72

Experiment Contact: 

Sussie Dalvin

Start Date: 

27 February, 2013 - 11:04

End Date: 

8 April, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000003611, EMLSAG00000003611-686377 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

Multidrug
Fragment description

Fragment length: 

493bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cgtctctcacgtcccttgtgtacaggaaatgtcttaaattatcaaattcatccagaaaaaataagagtgttggtgaaattgtcaacataatgtctgtagatgtgtctcgaattgcagatcttatgccttttttgacactcctttggtcttctccctttcaaattgttgtgagcgtaatttttatgtataacgaactaggttgggccattataggaggtactgggatccttctcatcactattcctgtgaatgctattttgagttatttcggtaaaaagttccaaatgaagcaaatgaaggcaaaggataataggaccaagatactaaacgaggttttaggtggaatacgtattctcaaactctatgcatgggagccatcgtacattaacaaaattgaaggaataagaggagtagaaattggaattttgaagaaagcagcatggttaacttcttttatgggatttatttggtcatccactccattcattgtagcg

Fragment Label ID: 

F72

Primer Group: 

b1361

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

12
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Forsøk J HIB startet 27 februar 2013

L F95

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

L

Experiment Contact: 

Sussie Dalvin

Start Date: 

17 April, 2013 - 14:29

End Date: 

10 June, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

PreAdult
Adult
Target description

Primary target ID: 

EMLSAG00000005923, EMLSAG00000005923-688689 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN17_g1274
Fragment description

Fragment length: 

457bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ataaacgaaagtgcagcattggcatccctaatgattgttgtcacgttagaagaaggacaacaaagattcgattgcagcttgtgtcacagtcgttataaacatcattcttccctatttcgacattttaaatccgcccatcacaaagaatatgaagaaagagtagctttgagggtaaaattaaaggctgaaagggatgctgcgatatcccggggggaaccttatttacgtagacgtaatagtaagcggggcttacgaagtgttaaaaagaacaaggagaatactacctcagaatctgttgcgaaggaagcagaatctcctacaaaatcaacaagtaagacatcgacagttaatgttaaacacgagtttaaatctccaaaatcaccagccaagagtcagaaaaaaaacatcattcatataaaaattcaacccaaagttgaggaacctcacatttcgtctt

Fragment Label ID: 

F95

Primer Group: 

b1477

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

18
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A group in experiment L. Exp has  been terminated, no obvious phenotype

K F80

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

k

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:49

End Date: 

13 May, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

36

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000004174, EMLSAT00000004174-700021 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Thyro g12693
Fragment description

Fragment length: 

436bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gcccaattaacggtattgactatggctataatcttgtaaacaataatgaaattgaggatttcaccattctaaatgcagtcccactaccggaaagtcctattccatccttaattgaggtaaaagaggaacccaagaaaagaaaacgacggccagagcccatgacggatgtttgcaggatatgtggggatattgcgccccctcataatcactacggtgcaattgcttgtttctcatgtagagctttcttcggaagaacagtgcataaaggaacccaggaaagatttcgctgtgtaagaggtaatccagatgaatccggacagtgtatgatcactaaagccacaagaacacattgtcagttttgtcgttatcgcggatgtctttctgccggaatgaaaccctcctatgtcacaactgccgaagagcgactgcaaaagaa

Fragment Label ID: 

F80

Primer Group: 

b1432

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

18
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Adult females were apparently normal

K F79

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

K

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:47

End Date: 

13 May, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

34

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000008430, EMLSAT00000008430-704277 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Zinc g18165
Fragment description

Fragment length: 

411bp

Fragment concentration: 

428ng/ul

Sense sequence: 

ctttattggaggatattggaggacgaagcaatcgatggcccaatggcattgttccatacacagtcagctcaagtttctcttcaagtcaaagacaaactatcatggattcaattcagcacatttcggatcgaacatgtgtcaaatttgttgaaagaacaaatgaagcgaattatgtatatattttcacgggtgaaagtggatgctatgctaatcttggctacaatagtgggagagcaagaagtgtccttcacttacagagcgatggctgtgtgtatttggggattgttgttcatgaactcctccacatccttggatttggtcatgaacaaacaagggctgatcgagaccaatatgttacaattaattggggaaatatacaaagtggaacctacagcaacttttggagagctc

Fragment Label ID: 

F79

Primer Group: 

b1430

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

21
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Adult females appeared normal

K F82

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

K

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:45

End Date: 

13 May, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

34

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000007918, EMLSAG00000007918-690684 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

430bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F82

Primer Group: 

b1408

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
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RNAi completed RNAi completed RNAi completed RNAi completed RNAi completed RNAi completed

K F96

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

K

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:28

End Date: 

13 May, 2013 - 00:00

Experiment Description: 

<p>22.4 All groups in exp K has eggstrings</p>
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

33

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

EMLSAG00000005837, EMLSAG00000005837-688603 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN18_g1340
Fragment description

Fragment length: 

467bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F96

Primer Group: 

b1479

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

14

Phenotype: 

No visible phenotype
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No obvious phenotype on the day of termination. No further testing

 

 

K F93

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

K

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:51

End Date: 

13 May, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

33

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000009301, EMLSAG00000009301-692067 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN14_g1154
Fragment description

Fragment length: 

429bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F93

Primer Group: 

b1471

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

16

Phenotype: 

No visible phenotype
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RNAi completed RNAi completed RNAi completed RNAi completed RNAi completed RNAi completed

K F76

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

K

Experiment Contact: 

Sussie Dalvin

Start Date: 

3 April, 2013 - 14:34

End Date: 

13 May, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

34

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN7_g423
LSalAtl2s685
Fragment description

Fragment length: 

422bp

Fragment concentration: 

433ng/ul

Sense sequence: 

gcctctttaggacatttgctgctaagatagagtagggagcttcatttgtgaggaacactttcaatctctcttttctaaaattggagcccaaagcgcggagaaggctagtcacagcaacttcttgaatggtgctacagttggcgttaacgacattgcagagattaatgaggtaaggggtacctgaaaaggtttcatccagtggaccaatcaatactgcaattaaagatcgatccttgatatccgtcgtctcgtccagtgtcatgaaaatatcccttccgtcaatttcgtcgctgatcttggaaagcatggactcactttccactttcaaggactgagtgatggcgtagcgggaaggaagggaccttccagtgtatttctggatgaagagggaaaagttgttgtattccacgatggagatggga

Fragment Label ID: 

F76

Primer Group: 

b1398

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

14

Phenotype: 

No visible phenotype
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RNA i  completed RNA i completed RNA i completed RNA i completed

H F59

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

H

Experiment Contact: 

Sussie Dalvin

Start Date: 

15 January, 2013 - 13:29

End Date: 

25 February, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000011506, EMLSAG00000011506-694272 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

g9531
Fragment description

Fragment length: 

484bp

Fragment concentration: 

400ng/ul

Sense sequence: 

acttgcttgtcttttctcggcatcaatatgcactcaacaatcagaagaggaatcatctaaattgcaactcagtgtatactatgagacaatgtgcccttattgcaaaaggcatatactggaagatttgcacccaacatatactcaactgagtgatatattggatgttcaattagtaccatatgggaatgctaagtcttcttcaagaccagacggaacaggctatacattttcttgtcagcatggccctacagagtgccttggaaatatggttcatgcctgtgcaattaaatatgtcaaattccccattctcatggatttcattgcatgtatgatggaaagaagtgatgtacccgttcttgcaaaaaaagagtgtgcaagtaaattggaaattgaatggactgaaattgaggagtgttccatgtcccttg

Fragment Label ID: 

F59

Primer Group: 

g9531_f_b1311

Fragment Producer: 

Heidi
Validation

Efficacy of Knock Down: 

F>0%
Phenotype

Number of Individuals (End): 

9
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Animals appeared normal. Nothing significant within the periode the experiment was performed. QPCR validation shows an upregulation of 20-100% depending on removal of one suspicious sample

RNAiE-IR25a/IR8a

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RNAiE

Experiment Contact: 

Anna Komisarczuk

Start Date: 

15 October, 2012 - 10:27

End Date: 

26 November, 2012 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Gene or target symbol: 

20261
IR25a
Fragment description

Fragment length: 

512bp

Fragment concentration: 

600ng/ul

Sense sequence: 

CATCATGCAATTCCTAATCCAGACCCTTTCCATTATCCTACCCTTCCTCATCCTCAAATCCAAATCTGCCAATGTTAATGTTTTATGGATCAATGATAAGAGTACTGGACTGATCAAAGACTCCGTGGATATAGCTCTTGACTATGTCAAAAAGAAAAACAATGTCGTTGTCACAAGTTTAGAGAGCGTGATGATGAATGGTAATGACATTGGGAATTCTACGGATAAAGTCTGTAACAAATTAAAGACAATGCTAGATGAGGGGAGATCTCCTGATATGATCCTTGATACAACAAGTGCTGGTACTTTGTCTGAGGTAGTCAAATCCTTGAGTTTATCCCTTGGCATCCCGACTTTAAGCACATCCTACGGGGATTTACGGGATGTAAGAGAATGGAGAAATTTAACATCTGATCAGGGGAAATACCTCATTCGAGTACGACCCCCAGGAGATCTACTTCCAAACATAATACGAAACATAATTACTTCAGACAATATTACAAACGCAGCCG

Antisense sequence: 

CGGCTGCGTTTGTAATATTGTCTGAAGTAATTATGTTTCGTATTATGTTTGGAAGTAGATCTCCTGGGGGTCGTACTCGAATGAGGTATTTCCCCTGATCAGATGTTAAATTTCTCCATTCTCTTACATCCCGTAAATCCCCGTAGGATGTGCTTAAAGTCGGGATGCCAAGGGATAAACTCAAGGATTTGACTACCTCAGACAAAGTACCAGCACTTGTTGTATCAAGGATCATATCAGGAGATCTCCCCTCATCTAGCATTGTCTTTAATTTGTTACAGACTTTATCCGTAGAATTCCCAATGTCATTACCATTCATCATCACGCTCTCTAAACTTGTGACAACGACATTGTTTTTCTTTTTGACATAGTCAAGAGCTATATCCACGGAGTCTTTGATCAGTCCAGTACTCTTATCATTGATCCATAAAACATTAACATTGGCAGATTTGGATTTGAGGATGAGGAAGGGTAGGATAATGGAAAGGGTCTGGATTAGGAATTGCATGATG

Fragment Label ID: 

57

Fragment Mixture Formula: 

F43 (IR25a) + F58 (IR8a)

Primer Group: 

IR25a_f_b1086

Fragment Producer: 

Heidi

Fragment Producer Contact: 

Anna Komisarczuk
Validation

Efficacy of Knock Down: 

F>39%
Phenotype

Number of Individuals (End): 

17

Phenotype: 

no visible phenotype
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Experiment E - IR25a and IR8a (new fragments). No visible phenotype at the end of experiment, but downregulation of IR25a is observed (qPCR with TaqMan probe).

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