no visible phenotype

NAE F2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

NAE

Experiment Contact: 

Christiane Trösse

Start Date: 

12 June, 2014 - 00:00

End Date: 

20 June, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

75

Developmental Stage CV: 

Nauplius I
Copepodid
Target description

Primary target ID: 

EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

LsIRP1A
Fragment description

Fragment concentration: 

10ng/ul

Fragment Label ID: 

F2
Validation

Efficacy of Knock Down: 

F>84%
Phenotype

Phenotype: 

no phenotype
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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

R F125

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

R

Experiment Contact: 

Frank Nilsen

Start Date: 

21 August, 2014 - 08:32

End Date: 

30 September, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN33_g12918
Fragment description

Fragment length: 

454bp

Fragment concentration: 

600ng/ul

Sense sequence: 

actttgtttctccctacgcgtggatttctgataatggaggcgaggaaatttggactttcctagcagaagaagataacataatgtgtcggatatgtcgttataagtgcccatttagccctccttctagaatgaaacggcacttagcatgccctacccatatcaggaatgttgagttattcagaaaacatgtaattcacgaggacatagactccggaaacttcaatacagacctcacaaagatgctcgtttcgtgtaacatacccctagccaccgtcgatcaccctaatttcaagaagttcttggagaaatatgttggtaagtccgttccaagtcgaaagtccacgattagattggttggagaagttagtaaggatgtcatctgcaaagttaaggaggaagttgagggtaaagatatcttcatagctgtagatgagaccaaggacaatcgagatcg

Fragment Label ID: 

F125

Primer Group: 

b1891

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

18
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Downregulation of gene with unknown function

U F187

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

U

Experiment Contact: 

Frank Nilsen

Start Date: 

3 December, 2014 - 13:51

End Date: 

14 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000011947, EMLSAG00000011947-694713 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

433bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gcctttcgttggatctacctgtggaacacgaaatttcaaccatgttccaggtctattacctggtcaaagtagtccagaagaattcccatggactgttttgatattaactcaaaagaatgaatttgtgggaacgggagttattgttcctgaaagatttgacaatcaagtacaattcaatacaagaaagattttgacggttgcgcacaaactcctcaattataatattaacgagttaaaagtgcgtgttttagactatgatgcaagtgattttaaattccctgaacaaagacggcatgaagaatttggagttgytcagtcctttattcatccccaatttgatcgaattcgattaattaatgacatagccgttctaaaggttgacagacccataaatttggagagacaaggaataagtgcagcctgctatccttcg

Fragment Label ID: 

F187

Primer Group: 

likb2942_T7_b2944

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>95%
Phenotype

Number of Individuals (End): 

16

Phenotype: 

Normal, hatching data yet to come
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File ru36.tif

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Fragment initaited by Gunnvør, may 2014

There is a lot of mistakes in the sequence

U F165

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

U

Experiment Contact: 

Frank Nilsen

Start Date: 

3 December, 2014 - 13:49

End Date: 

14 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000007327, EMLSAG00000007327-690093 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN49_7327
Fragment description

Fragment length: 

434bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gctggagcatctgctctctcagttgagatcccgtgggatacctgtgttgaagacagtgccgctacccttgtcactgacttcactccctatcccgtgagaatcgtttctggagaacaattgagtacaaaacttgtattgaatgtcctcaaggaaattgatggagaccttgaagttgaattgaaaatggccaagaaaggagctcttttcaatattcccattccttgcattgagatttctgagggattttttctcggatcctgcaagtacaaggttgctgaactattggaaaaatacggannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaactcttgtgccctgactttgttcccgaaggacaagagtgctctctccctatcaa

Fragment Label ID: 

F165

Primer Group: 

lik b2494_b2544

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>96%
Phenotype

Number of Individuals (End): 

10
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File ru30.tif

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DOwnregulation of gene with unknown function.

There are many nnn in the sequence. Primers for RNAi on both sides

U F160

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

U

Experiment Contact: 

Frank Nilsen

Start Date: 

3 December, 2014 - 13:47

End Date: 

14 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

28

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000010462, EMLSAG00000010462-693228 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN36_10462
Fragment description

Fragment length: 

514bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cccaatggtagacctatccgtgctactcattttctaacccataagtataattcttacactgatgctcatgaatactactctttattgggacatgaaatggcatcccattccatttctcattatgataacacatcatactgggtcagtttgaatgaatccgaatggagggatgaaatgatcggaatgaaagaaatgatgcatctctacggtaatatgaacatggcagacatcaaaggaacaagagctcctttccttcaaattggaggagatgttcaatttagagcattgagtgaggatttcgaatacgattgctctatgccatctagagcttttggatacacaaatctcgctaatggactctggccctacacacttgactatagatcaattcaagattgtcagcttgaaccttgccctctcgaatcttatcccaaagaatggatccaacccatgttggatcttgaggatttaagagtcggaattgatggaagtattcatggtcaaccctgtgcca

Fragment Label ID: 

F160

Primer Group: 

likb2470_T7_b2525

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

14
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File ru20.tif

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Downregulation of gene with unknown function.

T F177

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

t

Experiment Contact: 

Frank Nilsen

Start Date: 

30 October, 2014 - 13:41

End Date: 

9 December, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000000574, EMLSAT00000000574-696421 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment length: 

511bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cccggtcagtttccatggattgtaggactgtgggaaaagaatgggatatctcctttttgtggagggagtttactcaattttcgttggatcctcactgctgctcattgcgtatatcctgaaaattctgagacgtttcatcttcccatagctgtattaggagatcacaatacgagagatgtggaggatcctcataaagtcagaaccccaacatgtgctgttgaaatgcatcctggctattcaacttccaagcaggataatgacatagctcttttacgactctgcagtgatcttcaacagtttacgaaaagatattttgccattgatgttgcaagaccggaacaagaactcccggatgggacacctcttgtagttggaggttggggtgtactcagatttggtgcagtatctcccacagatatccttcgagctgttgttgtaaaaactgtcaatcatgatacatgtaacaatgcttatggatttattacgaaagctcatatatgtgctgggacgg

Fragment Label ID: 

F177

Primer Group: 

likb2605_T7_b2607

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>0%
Phenotype

Number of Individuals (End): 

19

Phenotype: 

No visible phenotype
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Looked quite normal, wait to see hatching

T F169

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

T

Experiment Contact: 

Sussie Dalvin

Start Date: 

30 October, 2014 - 13:38

End Date: 

9 December, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN53_5145
LsMvp1

Target type: 

UN53_5145
Fragment description

Fragment length: 

576bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cacatggagacgaagaagtgcgattggagagagatccgtttccattatatccaggagaagagttaaaggtcaaggtcacaccattggcaatcgttcatacctcaaatgcccttttattaaaagttataagaaatttcacggacgaaaatgatgttaaacgtgtggctggagatttatttttgtttgagggacctggaacttatattccaaggaaagaggtcgaggttcttaaaaccattacagcacaagttatccatgaaaatgaagcccttaaattaagtgcaagtcgtgaaaccatagatcgatccggaattaaacacgttgcaggtgaagaatggctcataagaaagcctggagcttatttgcctttggcttatgaaaatattgtggaagttgttaaggcacatataccccttccagatgtcgcaattttggtaagagcaaaggcaactttcaaggacaagtttggtgttgagagaaaaaatggagacaaatacttgattacctttgaagatacatcagcattcattcctgaggtacaagaagaaataattggcacagtggatgctatcacac

Fragment Label ID: 

F169

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>67%
Phenotype

Number of Individuals (End): 

13

Phenotype: 

normal
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Testing the fragment for Mvp1 knock-down.

T F168

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

T

Experiment Contact: 

Sussie Dalvin

Start Date: 

30 October, 2014 - 13:33

End Date: 

9 December, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN52_5383
LsMvp2/3
Fragment description

Fragment length: 

445bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cctcccacccgaatggtcgtaatacctccaggatcttattgtgttgtatcaaaccctgttgtaacaaaagatggtactattgagaaagatcgctttggccaaattaagttatctcatggagatgaagaaattcgattaaaaagagatccttttccattatatccaggagagaaattgaaagttgaagtgacgcctttaacaatcgtacattcatcaagtgcacttcttttaaaagttatcagaaattttactgatgaagacaaaactgagcgtsttgctggagatttatacttgttcgaaggtccaggaacttattttcctagaaaagaggtggaagttgttaaaactattactgctacagtcatacatgagaatgaagctttaaaactgagtgctgctcgtgaaactttagacagatctggttgtaagagagtggcaggggagg

Fragment Label ID: 

F168

Primer Group: 

likb2500_T7_b2513

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>72%
Phenotype

Number of Individuals (End): 

18

Phenotype: 

Normal
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Testing fragment for Mvp2/3. Due to high sequence identity, fragments will knock down both transcripts. 

T F167

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

T

Experiment Contact: 

Frank Nilsen

Start Date: 

30 October, 2014 - 13:27

End Date: 

9 December, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000001537, EMLSAT00000001537-697384 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN51_1537
Fragment description

Fragment length: 

354bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ttagtttcgcttaccttcggactggaaattcgtgactgcggttcccaaggagcagaaatcacaaaaatcgacttcccaacatgtattaaagagccctgtgttgttcataggggtgatcagctcaaagccaaactttatttgaaagccaaaaaagcaacagactatttggactgcgaactttcagctattatgagtggtattgagttgccttacccaggaggttgtgatgaagcagatgcctgccaatctcttcttgaaggagagtgccctgtagaagaaggagcagagcttatctatgacgtcagcatttatattgacaaaatcttccctacaattgttgtggatggaaaatgg

Fragment Label ID: 

F167

Primer Group: 

likb2498_T7_b2548

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

19

Phenotype: 

Some abnormal
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Downregulation of gene with unknown function

R F158

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

R

Experiment Contact: 

Sussie Dalvin

Start Date: 

21 August, 2014 - 15:28

End Date: 

30 September, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000009936, EMLSAT00000009936-705783 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F

Primer Group: 
Validation

Efficacy of Knock Down: 

F>95%
Phenotype

Number of Individuals (End): 

20

Phenotype: 

Normal
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This exp was performed to check the effects of knock down of the EMLSAT00000009936 gene: No obvious phenotype, normal animals and copepodit numbers

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