no visible phenotype

F230 Nauplia

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Phenotype CV: 

General

Experiment Batch ID: 

ExpA

Experiment Contact: 

Aina-Cathrine Øvergård
Sussie Dalvin

Start Date: 

1 February, 2017 - 10:26

End Date: 

13 February, 2017 - 10:27

Experiment Description: 

Gene knock-Down in nauplia, infestation of fish when copepodids and sampled 6 and 12 dpi (12 degrees).
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

550

Developmental Stage CV: 

Nauplius I
chalamus II/padI
Target description

Primary target ID: 

EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment length: 

192bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gaacaacgcaatctggacaagtgaaaaagagatgtttcggacgtagaccaagaccaagaccatcagcaaatcttagtgttaaggacttgatggatttaataaaaatgattatggaacttgcaaaaaagaaaggtgctgagaaaagaaatattgtttcaagtccaactatgactcatgttaaagtactctccg

Fragment Label ID: 

F230
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

240

Phenotype: 

No phenotype
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AG F230

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AG

Experiment Contact: 

Aina-Cathrine Øvergård
Sussie Dalvin

Start Date: 

16 August, 2016 - 10:26

End Date: 

23 September, 2016 - 10:27
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment length: 

192bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gaacaacgcaatctggacaagtgaaaaagagatgtttcggacgtagaccaagaccaagaccatcagcaaatcttagtgttaaggacttgatggatttaataaaaatgattatggaacttgcaaaaaagaaaggtgctgagaaaagaaatattgtttcaagtccaactatgactcatgttaaagtactctccg

Fragment Label ID: 

F230
Validation

Efficacy of Knock Down: 

F>100%
Phenotype

Number of Individuals (End): 

16

Phenotype: 

No phenotype
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File RAC61.tif

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AF F241

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AF

Experiment Contact: 

Heidi Kongshaug

Start Date: 

27 April, 2016 - 09:53

End Date: 

8 June, 2016 - 09:53
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000002443, EMLSAG00000002443-685209 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

T2_2443
Fragment description

Fragment length: 

538bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tgtagaccccactgtgcgccatactttttggactcaattaattggaggaatattcactgctataaccatatatggtgtgaatcaatcccaagttcagagattgctcaccatcggcagtttaaaacgttcccaaggatctctttggatacaatggcctataatggcttctcttagctttttgacgtcttttgcaggtcttactatgtatacatactataaggactgtgatcccgtacaaagtaaacgtattccaaggggagatcaattattggctctatttgtaatggatamaawgagkaattactacggrataacagggktatttgtggcaggratattctctggrtccttgagtactgtttcatcatccattaactcacttgctgcagtatctctcgaagactacattaaaccccttgttaaagtacctccgaaaatggaaactttggttttaaaattccttgttcttttttacggagttcttagtatcatacttgcttttctctgcgagtttttgccaagtggtattttgcaggcc

Fragment Batch ID: 

AF

Fragment Label ID: 

F241
Phenotype

Number of Individuals (End): 

21
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File RAF117.tif

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Knockdown av listefyll.

Nedregulering er ikke verifisert vha qPCR.

 

 

 

Z F197

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Frank Nilsen

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000000563, EMLSAT00000000563-696410 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

563
Fragment description

Fragment length: 

742bp

Fragment concentration: 

429ng/ul

Sense sequence: 

tggaacgcagtgtcagtgatctcattgggataaccccactaatatagcagcataaaacaccattgcccctcaggcttacgatgaattggtaaagcttgataagtgggccttctgaatgtgcatcgagatgtatcatctgcagaaaaggcttgcttcgaagatgttcaaagacttgtagaaacataaacaagagctctcgtggaagagggcaccactagaaactgaatgtaaaaagtacccggatcgtttacagtggtcgccaataagagtaatgaatagcgttccgattgaatcccgaagagcatatgggctaggcttaggggctccaatcgaaggaaatgagctgatgaccatatatctacctgaaaaatatttttgggttagtgacagtagaagatgtgttaaatgcaagagggttcatcctctatcattgatgctgaagaatcaactccaacc

Fragment Label ID: 

F197

Primer Group: 

b3250

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

26
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Image file: 

File rz144.tif

Gallery Image: 

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Unknown function of gene-

No validation of knockdown.

Z F215

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Frank Nilsen

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000000440, EMLSAG00000000440-683206 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

440
Fragment description

Fragment length: 

443bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tctcatcacctttgggcatatcatcatcctctttttgactctctactctaccacaatacaagctcttcaaaagattgagcaatttaacaaatatatagttctaacctctgctcaacatgtgatcgtctctcagagtgacagtaatttaatactcaatatcaccacaactaatgataactattatgaattgagactccataaagaggagaatagctttaacttcgaaatggattactacgtaggatttgtcgtgactcggaacagagacatgaaggagggacatgcctatggagtcctaaatgagaaaggcttcttccgaggaacatttgaggtaaatgatgacaaatactatatagaggatgcaaagagatacttggacaacgtgggaaaggatgttgtgggctttctttataaatcatcagatgtgcataatatcgaaaatgaattgaatgatgagttcaaaatggatcscccaccatctgagktatccactcgmtttggtgatcagtttattgaaaatgggccctattgtgttttaaacatcgaagtagataaatctctctactatagctttgatcgaaatattcaggacattaagcaatttgtgttcatgactgtcatgggaaccaataaaatatatcgggaagcagatctcgtaacaatcgacggtcaccccattcagtttgttgttggaagactccaagttaacacaccacagtcttgcaattccactggtagaaggtcgtacaggtgctcgaatgaaaaatcctcagttgaaccaaagatattcattgaggatttttctttcgcccccgctccgcatactgactactgcttatcatatgttttctccgcacaaaactttggagccacattgggaatagcatasgtrggrggggtgkgsgagccacaaattatagatggggatcgaaactataatgtgggtttcattacaacaggaggattccgaactcagtatcaaacagaacttacactagcgcatgaattcggtcacagccttggctctgcacacgactcatttggtgatggttgtctggatcctcaatatatgga

Fragment Label ID: 

F215

Primer Group: 

b3313

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

16
Upload

Image file: 

File rz124.tif

Gallery Image: 

Image Quality: 

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Unknown function of gene.

Downregulation was not verified by qPCR.

Z F205

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Frank Nilsen

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAG00000000367, EMLSAG00000000367-683133 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

367
Fragment description

Fragment length: 

393bp

Fragment concentration: 

465ng/ul

Sense sequence: 

atgaaggctttcgtcgctgttctttctaccctcatcgctgttgcttcaagcacagtcctccccggtgcctatgcccatggacatggattgtacggttatgccggatatggtgcccccatagctcaccatggtgtttatgctcmcgctcctgccgcctacccttatgcctacgctgatgcctaccccgttgccgaaccctacatccacgaagaagttgcagctgaggaatacgttcacgaagaggtccctgctgaggaatacgttcatcaagagatcgctgctgaggaatactcccacgaggaggtccctgctgaggaatatgttcatgaggaggtcgccgctgagccctacattcaccaagaacccgtctatgctcctgcttattactccgga

Fragment Label ID: 

F205

Primer Group: 

b3244

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

19
Upload

Image file: 

File rz84.tif
File rz90.tif

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Image Quality: 

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Unknown function of gene.

Downregulation not verified by qPCR.

Z F219

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Heidi Kongshaug

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

CMi2471 (negative control), CMi2471 (PCR_product) Gadus morhua

Gene or target symbol: 

CMi2471
Fragment description

Fragment length: 

428bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gcggccgctctagaactagtggatcccccgggctgcaggaattcggcacgagggtttttttttattattttgattttgaatcagcattttaaaaagacaaacatgcagcattacatcacacacagaaaaacaacatctactggggcaagcacgtagaaggacccaggttcatgttgataatgttaacattgcctgatgacttttcagtaattaacttgacaatgggttggtttttggaatccatagacatgtggtagacttcgcatctaaacactctgccgtctttccaaccatcagagctgaaggtaagttggccataagtggaaaacaagtcgccgttttcaattacgtttgttgttttgtgtgaataaagagcatttccatctattgtcaaattatcaaccagccaatgcaccaaaatgtccgcg

Fragment Label ID: 

F219

Primer Group: 

b3396

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

19
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Control fragment CMi 2471 for injections.

Testing possible new control fragment. No lice were kept. Downregulation not validated. No pictures were taken.

Z F218

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Heidi Kongshaug

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

CTH210 (negative control), CTH210 (PCR_product) Gadus morhua

Gene or target symbol: 

CTH210
Fragment description

Fragment length: 

482bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gtactgaaacggacagcagtggcaactatgtgttgtcctggttccagcagaagagtggttcaccgccaaagtttttacttaccacaacaagcagtagggcaagtggcgttcccagtaggtttacttattctggtacaagaaatgacaaaacagagtatctactgattaatggcattcaagatgaagatgaggcgacatactactgtgcgtgtgtcagctgtggtacagactctccaaccttctttggtggtggcactgaagtcattcttgctggttccccgtccccccccagtctggagctgatggtggccacccagcctcctgtcccggggctgggggggcccactctggtgtgcctggctaagggcttccacccggccggggccgccctgtcctggtccgaggacggagcctctgtgaggggcgaggaggtccgggggggcgtggcccagcggcagcctgatggaagctactcccttagc

Fragment Label ID: 

F218

Primer Group: 

b3398

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

17
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Control fragment CTH 210 for injections.

testing new control.

No pictures taken. Lice were not kept. No validation of downregulation.

NAH F186

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

NAH

Experiment Contact: 

Christiane Trösse

Start Date: 

21 November, 2014 - 00:00

End Date: 

12 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

300

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

EMLSAG00000005336, EMLSAG00000005336-688102 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

FLVCR2
Fragment description

Fragment length: 

533bp

Fragment concentration: 

10ng/ul

Fragment Label ID: 

F186

Primer Group: 

b2654

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

23
Upload

Image file: 

Image icon L1_FLVCR2.JPG

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis, and with elevated expression from the parasitic copepodid stage. Infection of fish with knockdown copepodids (3 fish with 80 lice) and lice count after 16 and 42 days. No significant difference in number of lice on fish at termination. qPCR validation not done yet.

NAH F154

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

NAH

Experiment Contact: 

Christiane Trösse

Start Date: 

21 November, 2014 - 00:00

End Date: 

12 January, 2015 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

300

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

EMLSAG00000003292, EMLSAG00000003292-686058 (gene) Lepeophtheirus salmonis

Gene or target symbol: 

HCP1
Fragment description

Fragment length: 

541bp

Fragment concentration: 

10ng/ul

Fragment Label ID: 

F154

Primer Group: 

b2341

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

33
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Image file: 

Image icon J6_HCP1.JPG

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis, and with elevated expression from the parasitic copepodid stage. Infection of fish with knockdown copepodids (3 fish with 80 lice) and lice count after 16 and 42 days. No significant difference in number of lice on fish at termination. qPCR validation not done yet.

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