E F35

Submitted by Heidi Kongshaug on 4 February, 2015 - 14:37

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

e

Experiment Contact:

Sussie Dalvin

Start Date:

15 October, 2012 - 14:33

End Date:

26 November, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000011088, EMLSAT00000011088-706935 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g18363_LsRictor

Fragment description

Fragment length:

421bp

Fragment concentration:

600ng/ul

Sense sequence:

aatttgagggctcgattcctggagaataacaacgaagacgatgagttggatttgtctgaggggagttgtgttggggagaacgtgattgaggctctcaagtacctgtcctccaaaggagggtccaagggaaagaaactggcctatctgaacgcactcgtcaaagttctcttggaacagcggaaggattccttggaggaggatctgtctctgggcttgaggctcaaggaaatcatgctttgtctccgtattcctttgacccatgaagcggcagaagtgcgagctgcgactcttcgtgtgtttcgatacattgtggatcgtccttgcagagtccaggatttccttcaaatcaatgctcacctccttgtttcaagatgcatcgacatcgacctggacaatcgagtagaaagactgcaagccctgc

Fragment Label ID:

F35

Primer Group:

Rictor_f_b920

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>35%

Phenotype

Number of Individuals (End):

23

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Rictor: 23 females recovered (59%). Normal, blood in the gut and eggstrings that hatch.A few with gonade syndrome in one side F35: Indications of a downregulation

D F32

Submitted by Heidi Kongshaug on 4 February, 2015 - 13:46

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:42

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

NLG3408_TSC1

Fragment description

Fragment length:

549bp

Fragment concentration:

600ng/ul

Sense sequence:

ctgctctgagagatcaactccagcttgtcaaatcagaaattgatgatttacaccgtcaaattgaggaaataagaagggaaaaacatctgatagaggaagaaaggtcatttactgcaagaaatagaggtcatgaaatcgaaaaaaggtctgctgagattagatctttaaaaatcagtgcacaaaaatctgaagaaataattgatgagctcaaccatgctgtagtagaaggaaggaaggagttggatacctccagggctcaatttttcctaatgaaagctgaatgcgaggttttacatcaaaaagaaagcgcaagaatgatgacggaattggaattggttcgactgagaaaggaacttgtcatgatgggtgagcttcagtcaaaatataaggagcggttggataatcctgctaacaacatggaatctgagaccatgaatctctttaagcaggcttatgaaagggatctacagactatggccagagctctggagaacaaaacatttgaagtagagacattcaagtgtcgtatcaccgagttggactccttgc

Fragment Label ID:

F32

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>33%

Phenotype

Number of Individuals (End):

7

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RD83.tiff

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Tsc1, high mortality but looks normal TSC-1: Not significantly downregulated but only checked with primers inside ds fragment

NAG F171

Submitted by Christiane Trösse on 30 January, 2015 - 11:09

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000007383, EMLSAT00000007383-703230 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsFepo

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F171

Primer Group:

Validation

Efficacy of Knock Down:

F>63%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F170

Submitted by Christiane Trösse on 30 January, 2015 - 11:05

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000005522, EMLSAT00000005522-701369 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsDcytB

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F170

Primer Group:

Validation

Efficacy of Knock Down:

F>85%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F155

Submitted by Christiane Trösse on 30 January, 2015 - 10:58

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000008285, EMLSAT00000008285-704132 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsHRG1

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F155

Primer Group:

Validation

Efficacy of Knock Down:

F>79%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F154

Submitted by Christiane Trösse on 30 January, 2015 - 10:47

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000003292, EMLSAT00000003292-699139 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsHCP1

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F154

Primer Group:

Validation

Efficacy of Knock Down:

F>53%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F153

Submitted by Christiane Trösse on 30 January, 2015 - 10:37

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000009982, EMLSAT00000009982-705829 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsFer4

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F153

Primer Group:

Validation

Efficacy of Knock Down:

F>92%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F152

Submitted by Christiane Trösse on 30 January, 2015 - 10:31

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000001594, EMLSAT00000001594-697441 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsFer3

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F152

Primer Group:

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F51

Submitted by Christiane Trösse on 30 January, 2015 - 10:20

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000006305, EMLSAT00000006305-702152 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsFer2

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F51

Primer Group:

Validation

Efficacy of Knock Down:

F>91%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.

NAG F50

Submitted by Christiane Trösse on 30 January, 2015 - 10:12

Phenotype CV:

no visible phenotype

General

Experiment Batch ID:

NAG

Experiment Contact:

Christiane Trösse

Start Date:

19 June, 2014 - 00:00

End Date:

27 June, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

unknown

Number of Individuals (Start):

75

Developmental Stage CV:

Nauplius I

Copepodid

Target description

Primary target ID:

EMLSAT00000010792, EMLSAT00000010792-706639 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsFer1

Fragment description

Fragment concentration:

10ng/ul

Fragment Label ID:

F50

Primer Group:

Validation

Efficacy of Knock Down:

F>94%

Phenotype

Phenotype:

no phenotype

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Sampling 27.06.2014

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RNAi knockdown in nauplii of genes involved in iron or heme uptake, transport, storage or homeostasis.