RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
101 RNAi_AE 3422 EMLSAG00000003422, EMLSAG00000003422-686188 (gene) Lepeophtheirus salmonis preadult II male, preadult I and II female 78 Lepeophtheirus salmonis 23 February, 2016 - 10:15
102 RNAi_AE 9341 EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis preadult I male, preadult I and II female 95 Lepeophtheirus salmonis 23 February, 2016 - 10:18
103 AC F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult Control 30/28 Lepeophtheirus salmonis 22 March, 2016 - 09:10
104 AC F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
Adult female reproduction, no eggstrings 31/21 Lepeophtheirus salmonis 31 March, 2016 - 09:56
105 LsRXR EMLSAT00000001919, EMLSAT00000001919-697766 (mRNA) Lepeophtheirus salmonis
LsRXR
Adult, Copepodid female reproduction, reduced offspring viability, shorter eggstrings 50/38 Lepeophtheirus salmonis F>60.00% 11 April, 2016 - 12:18
Characterization of a novel RXR receptor in the salmon louse...
106 F235 EMLSAT00000003009, EMLSAT00000003009-698856 (mRNA) Lepeophtheirus salmonis
EMLSAG00000003009
Adult, Adult 30/24 Lepeophtheirus salmonis 12 April, 2016 - 11:07
107 AF F239 (F233+F234) EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis, EMLSAT00000002881, EMLSAT00000002881-698728 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010949, EMLSAG00000002881
Adult, Adult 30/25 Lepeophtheirus salmonis 12 April, 2016 - 11:21
108 AC F231 EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
Adult, Adult 30/25 Lepeophtheirus salmonis F>99.50% 12 April, 2016 - 11:31
109 AF F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:33
110 AF F228 EMLSAG00000006953, EMLSAG00000006953-689719 (gene) Lepeophtheirus salmonis
6953
PreAdult2 30/15 Lepeophtheirus salmonis 16 July, 2016 - 11:45

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