RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
91 NAI F227 EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis
LsPGES3
Nauplius1, Copepodid 100 Lepeophtheirus salmonis 14 January, 2016 - 16:59
92 NAI F227, F226 EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis
F227, F226
Nauplius1 100 Lepeophtheirus salmonis 15 January, 2016 - 13:22
93 NAJ Dopaminreceptor EMLSAG00000003021, EMLSAG00000003021-685787 (gene) Lepeophtheirus salmonis Nauplius1 100 Lepeophtheirus salmonis 15 January, 2016 - 13:31
94 NAK F205 LsPGES1 EMLSAT00000006733, EMLSAT00000006733-702580 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 10:42
95 NAK F226+F227+F205 EMLSAT00000000441, EMLSAT00000000441-696288 (mRNA) Lepeophtheirus salmonis, EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis, EMLSAT00000006733, EMLSAT00000006733-702580 (mRNA) Lepeophtheirus salmonis
LsPGES2, LsPGES3, LsPGES1
Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 10:58
96 NAK F227 EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 11:01
97 NAK F226+F227 EMLSAT00000000441, EMLSAT00000000441-696288 (mRNA) Lepeophtheirus salmonis, EMLSAT00000012943, EMLSAT00000012943-708790 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid 100 Lepeophtheirus salmonis 28 January, 2016 - 11:04
98 RNAi_AE F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua preadult II male, preadult I and II female 96 Lepeophtheirus salmonis 23 February, 2016 - 10:01
99 RNA_AE F255 lcl|LsalIR338-Anna|LsalIR338, lcl|LsalIR338-Anna|LsalIR338.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR331-Anna|LsalIR331, lcl|LsalIR331-Anna|LsalIR331.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR324-Anna|LsalIR324, lcl|LsalIR324-Anna|LsalIR324.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR339-Anna|LsalIR339, lcl|LsalIR339-Anna|LsalIR339.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR340-Anna|LsalIR340, lcl|LsalIR340-Anna|LsalIR340.mrna1 (mRNA) Lepeophtheirus salmonis, lcl|LsalIR8a.2-Anna|LsalIR8a.2, lcl|LsalIR8a.2-Anna|LsalIR8a.2.mrna1 (mRNA) Lepeophtheirus salmonis preadult II male, preadult I and II female 96 Lepeophtheirus salmonis 23 February, 2016 - 10:09
100 RNAi_AE 5667 EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis preadult I male, preadult I and II female 77 Lepeophtheirus salmonis 23 February, 2016 - 10:12

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