RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
341 AR F244 EMLSAG00000010957, EMLSAG00000010957-693723 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/15 Lepeophtheirus salmonis 2 May, 2019 - 10:50
342 AR F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 No visible phenotype
no visible phenotype
30/10 Lepeophtheirus salmonis 21 November, 2018 - 10:10
343 AQ F375 EMLSAG00000008959 EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis
EMLSAG00000008959
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/5 Lepeophtheirus salmonis 29 May, 2018 - 14:58
344 AQ F374 EMLSAG00000011382 EMLSAT00000011382, EMLSAT00000011382-707229 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011382
PreAdult2 No visible phenotype
no visible phenotype
30/9 Lepeophtheirus salmonis 29 May, 2018 - 14:02
345 AQ F373 EMLSAG00000004269 EMLSAT00000004269, EMLSAT00000004269-700116 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
30/7 Lepeophtheirus salmonis 29 May, 2018 - 13:55
346 AQ F372 EMLSAG00000011562 EMLSAT00000011562, EMLSAT00000011562-707409 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011562
PreAdult2 No visible phenotype
no visible phenotype
30/13 Lepeophtheirus salmonis 29 May, 2018 - 14:37
347 AQ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 Control 30/5 Lepeophtheirus salmonis 6 June, 2018 - 09:56
348 AP F367 EMLSAG00000005299 EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005299
PreAdult2 slightly shorter gonade and abdominal segment
reproduction, female reproduction, shorter eggstrings
30/24 Lepeophtheirus salmonis F>16.00% 29 May, 2018 - 12:27
349 AP F366 EMLSAT00000001458 EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
EMLSAG00000001458
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>77.00% 29 May, 2018 - 11:26
350 AP F365 EMLSAG00000003179 EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis PreAdult2 slightly shorter gonade and abdominal segment 30 Lepeophtheirus salmonis F>47.00% 29 May, 2018 - 12:46

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