RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending Publication
201 F230 Nauplia EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Nauplius I, chalamus II/padI No phenotype
no visible phenotype
550/240 Lepeophtheirus salmonis F>98.50% 21 February, 2017 - 10:25
202 AG F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No phenotype
no visible phenotype
30/16 Lepeophtheirus salmonis F>99.90% 21 February, 2017 - 10:15
203 Functional characterization of a heme Peroxidase (Nauplia) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
Nauplius1, Copepodid Decreased swimming activity
Motility, reduced
400/400 Lepeophtheirus salmonis F>89.00% 21 February, 2017 - 10:04
Molecular characterization and functional analysis of a...
204 Functional characterization of a heme Peroxidase (male) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
padII/ad, Adult Fall off the fish
survival, reduced
42/1 Lepeophtheirus salmonis F>98.00% 21 February, 2017 - 09:56
Molecular characterization and functional analysis of a...
205 Functional characterization of a heme Peroxidase (female) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
PreAdult1, PreAdult2/Adult Fall off the fish
survival, reduced
42 Lepeophtheirus salmonis F>78.00% 16 February, 2017 - 17:49
Molecular characterization and functional analysis of a...
206 Testis-specific 2489 AG F271 EMLSAT00000002489, EMLSAT00000002489-698336 (mRNA) Lepeophtheirus salmonis adult male no visible phenotype Lepeophtheirus salmonis 26 January, 2017 - 15:15
207 Mucin-like spermatophore wall protein 1 (AG F269) EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis
MLSWP1
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>95.00% 26 January, 2017 - 15:07
A novel gene-family involved in spermatophore generation in...
208 Mucin-like spermatophore wall protein 2 (AG F270) EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis
MLSWP2
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>80.00% 26 January, 2017 - 14:55
A novel gene-family involved in spermatophore generation in...
209 AF F241 EMLSAG00000002443, EMLSAG00000002443-685209 (gene) Lepeophtheirus salmonis
T2_2443
PreAdult2, Adult no visible phenotype 30/21 Lepeophtheirus salmonis 16 July, 2016 - 12:00
210 AF F264 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000006733, EMLSAG00000006733-689499 (gene) Lepeophtheirus salmonis
441, 12943, 6733
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:56

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