RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
281 EMLSAT00000004874_Var2Frag1 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Nauplius1, Copepodid no visible phenotype Lepeophtheirus salmonis F>77.00% 14 June, 2018 - 13:19
282 EMLSAT00000003179 cop + infection EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis
Csdc2
Nauplius1, Copepodid no proper visible phenotype but maybe quicker deve
no visible phenotype
180/45 Lepeophtheirus salmonis 14 June, 2018 - 13:09
283 EMLSAT00000002095_copepodites EMLSAT00000002095, EMLSAT00000002095-697942 (mRNA) Lepeophtheirus salmonis
Astacin
Copepodid, Copepodid host related phenotype, modulation of immune response 420/50 Lepeophtheirus salmonis F>94.00% 14 June, 2018 - 13:12
284 EMLSAT00000001458 cop + infection_b EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
100 Lepeophtheirus salmonis 6 August, 2018 - 16:19
285 EMLSAT00000001458 cop + infection EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
60 Lepeophtheirus salmonis 14 June, 2018 - 11:36
286 EMLSAG00000013035 EMLSAT00000013035, EMLSAT00000013035-708882 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/20 Lepeophtheirus salmonis 12 April, 2016 - 10:26
287 E F51 (Ferr_LH) EMLSAT00000006305, EMLSAT00000006305-702152 (mRNA) Lepeophtheirus salmonis
ferr_LH
PreAdult2, Adult Most lice didn’t have egg strings at termination (
female reproduction, no eggstrings
39/23 Lepeophtheirus salmonis F>78.00% 28 June, 2013 - 11:43
288 E F50 (Ferr_sub) EMLSAG00000010792, EMLSAG00000010792-693558 (gene) Lepeophtheirus salmonis
ferr_sub
PreAdult2, Adult Most lice didn’t have egg strings at termination (
female reproduction, no eggstrings
38/18 Lepeophtheirus salmonis F>85.00% 21 February, 2013 - 11:54
289 E F49b EMLSAT00000002641, EMLSAT00000002641-698488 (mRNA) Lepeophtheirus salmonis
ILR frag2 halv dose
PreAdult2 40/25 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 14:59
290 E F49a EMLSAT00000002641, EMLSAT00000002641-698488 (mRNA) Lepeophtheirus salmonis
ILR frag2
PreAdult2 35/22 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 14:57

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