no visible phenotype

AR F385 EMLSAT00000007048

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RNAiAR

Experiment Contact: 

Christiane Eichner

Start Date: 

16 August, 2018 - 09:00

End Date: 

20 September, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000007048
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

EMLSAG00000007048
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F385

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

12

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000007048. 

AR F249 EMLSAT00000010968

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RNAiAR

Experiment Contact: 

Christiane Eichner

Start Date: 

16 August, 2018 - 09:00

End Date: 

20 September, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000010968
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000010968, EMLSAT00000010968-706815 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

EMLSAG00000010968
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F249

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

11

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000010968. 

AR F0

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AR

Experiment Contact: 

Christiane Eichner

Start Date: 

16 August, 2018 - 09:00

End Date: 

20 September, 2018 - 09:00

Experiment Description: 

control RNAiAR
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol: 

CPY185
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F0

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

10

Phenotype: 

No visible phenotype
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control group 

AR F244 EMLSAT00000010957

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RNAiAR

Experiment Contact: 

Christiane Eichner

Start Date: 

16 August, 2018 - 09:00

End Date: 

20 September, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000010957
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000010957, EMLSAT00000010957-706804 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

EMLSAG00000010957
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F244

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

15

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000010957. 

ExpD EMLSAT00000009293

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

ExpC

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

11 October, 2017 - 10:05

End Date: 

20 October, 2017 - 10:09

Experiment Description: 

Salmon at 12 degrees were infested in single tanks with 5 female and 5 male knock-down adult lice. The lice were put on Fish 5 days after injection. Sampling 4 days post infestation. Immun gene response were measured in salmon skin at attachment site.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

50

Developmental Stage CV: 

Adult
Adult
Target description

Primary target ID: 

EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Unknown

Target type: 

mRNA
Fragment description

Fragment length: 

387bp

Fragment concentration: 

600ng/ul

Sense sequence: 

attcttaatgaagaatctggtgagggaaatgtcacagtcaccgtggtagatacaatggatagaagttcctttgtcatgaactccgatgatgagagtgctggtcttccagttccccttagtgaagtcgtggaaacccccaacaatgactatataatgaataatcctatcgttttaaatgagaaaaacgatgttattctgaatgacgatgttattcttagtggtgaagaccttactctgattgaaaatcccactttatcattggatgatcttctagttgaagaagaagaaagtgatgatgttattttgaactaaatgtcacaatatctttctgtatgtctaataaacagataataaatatatataacccaatgtttcattgaaaagtcc

Fragment Label ID: 

E9293

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

50
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påiåp

EMLSAT00000007048 cop + infection

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

network_moult nauplia + infection

Experiment Contact: 

Christiane Eichner

Start Date: 

9 May, 2018 - 09:00

End Date: 

1 June, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000007048 in nauplia1 and infection of salmon with copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

180

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis

Target type: 

mRNA

External DBref: 

Fragment description

Fragment concentration: 

20ng/ul

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

EMLSAT00000007048

Fragment Mixture Formula: 

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Christiane Eichner
Phenotype

Number of Individuals (End): 

45

Phenotype: 

no visible phenotype
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network middle old/moult steelblue cluster (most preserved in middle)

EMLSAT00000005299 cop + infection

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

network_moult nauplia + infection

Experiment Contact: 

Christiane Eichner

Start Date: 

9 May, 2018 - 09:00

End Date: 

1 June, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000005299 in nauplia1 and infection of salmon with copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

180

Developmental Stage CV: 

Nauplius1
chalimus and preadult
Target description

Primary target ID: 

EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Emc3

Target type: 

mRNA
Fragment description

Fragment length: 

424bp

Fragment concentration: 

20ng/ul

Sense sequence: 

TCAAGGGTAACATTACTAACATGTTGCCAATGATTGTAATTGGAGGATGGATCAACTGGCATTTCTCAGGCTTTGTCACTACCAAAATCCCTTTTCCCCTCACTCTTCGTTTCAAGCCTATGCTTCAAAGAGGCATTGAACTCATGAGTCTAGATGCTTCATGGGTCTCCTCCGCCTCCTGGTACTTTCTTAACGTTTTCGGACTACGATCTATTTATGACTTAGTTCTCGGAGAAAATAATGTTGCGGATCAATCAAGAATCATGCAGGACCAGATGATGATGCCTGCCAACGGAATGGCTAKCGACTATAAACAGGCCTTCAAAGGGGAATGGGAAGCACTGGAAATCACTGAACATGCTTGGGCTCTCAAAAATGCTGAGCAGGAAATGATCTCTGCTTTTGAAAGAAGAAAGGCGGAAGG

Fragment Label ID: 

F367

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Christiane Eichner
Phenotype

Number of Individuals (End): 

52

Phenotype: 

no proper visible phenotype but maybe quicker development than control
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network middle old/moult violet cluster (lethal + chitin genes)

Nauplii I 5242-2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

N

Experiment Contact: 

Anna Komisarczuk

Start Date: 

1 February, 2016 - 14:40

End Date: 

10 February, 2016 - 01:41

Experiment Description: 

Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Developmental Stage CV: 

Nauplius I
Target description

Primary target ID: 

EMLSAG00000005242, EMLSAG00000005242-688008 (gene) Lepeophtheirus salmonis

Target type: 

External DBref: 

Fragment description

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F306

Fragment Mixture Formula: 

Fragment Producer: 

Validation

Efficacy of Knock Down: 

F>80%
Phenotype

Phenotype: 

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no phenotype

Nauplii I 7798-1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

N

Experiment Contact: 

Anna Komisarczuk

Start Date: 

1 February, 2016 - 14:40

End Date: 

10 February, 2016 - 01:41

Experiment Description: 

Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Developmental Stage CV: 

Nauplius I
Target description

Primary target ID: 

EMLSAG00000007798, EMLSAG00000007798-690564 (gene) Lepeophtheirus salmonis

Target type: 

External DBref: 

Fragment description

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F305

Fragment Mixture Formula: 

Fragment Producer: 

Validation

Efficacy of Knock Down: 

F>80%
Phenotype

Phenotype: 

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no phenotype

Nauplii I 3059-1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

N

Experiment Contact: 

Anna Komisarczuk

Start Date: 

1 February, 2016 - 14:40

End Date: 

10 February, 2016 - 01:41

Experiment Description: 

Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Developmental Stage CV: 

Nauplius I
Target description

Primary target ID: 

EMLSAG00000003059, EMLSAG00000003059-685825 (gene) Lepeophtheirus salmonis

Target type: 

External DBref: 

Fragment description

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F302

Fragment Mixture Formula: 

Fragment Producer: 

Validation

Efficacy of Knock Down: 

F>80%
Phenotype

Phenotype: 

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no phenotype

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