RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
201 AP F367 EMLSAG00000005299 EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005299
PreAdult2 slightly shorter gonade and abdominal segment
reproduction, female reproduction, shorter eggstrings
30/24 Lepeophtheirus salmonis F>16.00% 29 May, 2018 - 12:27
202 AP F365 EMLSAG00000003179 EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis PreAdult2 slightly shorter gonade and abdominal segment 30 Lepeophtheirus salmonis F>47.00% 29 May, 2018 - 12:46
203 AG-F286 EMLSAT00000011173, EMLSAT00000011173-707020 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011448, EMLSAT00000011448-707295 (mRNA) Lepeophtheirus salmonis PreAdult2 Slightly curly eggstrings. 29/19 Lepeophtheirus salmonis 16 March, 2017 - 11:15
204 LsHSCARB - a putative intestinal heme receptor EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis PreAdult2 Shorter egg-strings, 60% less heme, lower hatching
feeding, reduced nutrient uptake
30/15 Lepeophtheirus salmonis F>95.00% 4 December, 2017 - 10:55
A scavenger receptor B (CD36)-like protein is a potential...
205 AR F376 EMLSAT00000004347 EMLSAG00000004347, EMLSAG00000004347-687113 (gene) Lepeophtheirus salmonis
EMLSAG00000004347
PreAdult2 shorter eggstring 30/10 Lepeophtheirus salmonis 21 September, 2018 - 19:44
206 X F209 EMLSAT00000011090, EMLSAT00000011090-706937 (mRNA) Lepeophtheirus salmonis PreAdult2 Short egg-strings. 30/11 Lepeophtheirus salmonis 14 March, 2017 - 21:36
207 S F184 EMLSAT00000007308, EMLSAT00000007308-703155 (mRNA) Lepeophtheirus salmonis
ChitinSynthase2(b)
Adult Short eggstrings 30/18 Lepeophtheirus salmonis 15 January, 2015 - 15:11
208 Nauplii I 12262+22100+4547+12296+12453 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis, EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis Nauplius I reproductive defect as adults
reproduction
Lepeophtheirus salmonis 2 May, 2018 - 14:43
209 F159 EMLSAG00000001069, EMLSAG00000001069-683835 (gene) Lepeophtheirus salmonis
Peroxidase
PreAdult1 female, PreAdult2 male Reduced survival 90/11 Lepeophtheirus salmonis F>90.00% 11 June, 2014 - 11:16
210 P F149 EMLSAG00000010881, EMLSAG00000010881-693647 (gene) Lepeophtheirus salmonis PreAdult2, Adult Reduced survival 39 Lepeophtheirus salmonis 11 June, 2014 - 13:50

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