AC F0S

Submitted by Heidi Kongshaug on 31 March, 2016 - 09:56

Phenotype CV:

General

Experiment Batch ID:

AC

Experiment Contact:

Heidi Kongshaug

Start Date:

14 December, 2015 - 09:53

End Date:

25 January, 2016 - 09:53

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

31

Developmental Stage CV:

Adult

Target description

Primary target ID:

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol:

CPY short version

Fragment description

Fragment length:

320bp

Fragment concentration:

600ng/ul

Sense sequence:

cgttctgctcctcggagctgtcttcgctgaggaggacaagatcgtcggagggtatgagtgtacgaggcactcccaggcccaccaggtgtctctgaactctggataccacttctgtggaggctccctggtcagcaaggactgggtggtgtctgctgctcactgctacaagtcccgtattgaggtgcgtctgggcgagcaccacatcagggtcaacgagggaaccgagcagttcatctcctcctccagcgtcatccgtcaccccaactacagctcctacaacatcgacaacgacatcatgctgatcaagctgagcaagcccg

Fragment Label ID:

F0

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

21

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RAC27.tif

RAC29.tif

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Testing new and shorter fragment of CPY

New fragment is 320 bp and called F0S

C F4 (CopB2)

Submitted by Christiane Trösse on 24 July, 2015 - 16:11

Phenotype CV:

General

Experiment Batch ID:

C

Experiment Contact:

Christiane Trösse

Start Date:

14 August, 2012 - 09:00

End Date:

24 September, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>82%

Phenotype

Number of Individuals (End):

0

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4a.JPG

4b.JPG

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Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F4 (CopB2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:44

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>65%

Phenotype

Number of Individuals (End):

5

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9b.JPG

9c.JPG

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Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

D F39

Submitted by Liv Sandlund on 12 December, 2014 - 10:54

Phenotype CV:

General

Experiment Batch ID:

D

Experiment Contact:

Heidi Kongshaug

Start Date:

24 August, 2012 - 10:39

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis

Gene or target symbol:

EcR

Target type:

consensus_mRNA

Fragment description

Fragment length:

820bp

Fragment concentration:

600ng/ul

Fragment ID:

EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis

Fragment Label ID:

F39

Primer Group:

b1025 LsEcR Frag2 F

Validation

Efficacy of Knock Down:

F>0%

Phenotype

Number of Individuals (End):

16

Phenotype:

No eggstring production

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No production of eggstrings Knockdown was not significant after 40 days

E F51 (Ferr_LH)

Submitted by Christiane Trösse on 28 June, 2013 - 11:43

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Christiane Trösse

Start Date:

15 October, 2012 - 00:00

End Date:

26 November, 2012 - 00:00

Experiment Description:

Ferr:_LH

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000006305, EMLSAT00000006305-702152 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

ferr_LH

Fragment description

Fragment length:

676bp

Fragment concentration:

600ng/ul

Sense sequence:

ctatttattgacaggctcaacgccgtgctttaagatgatcaaacaattattgcttttgtcaattttagttgcaagctgtgtgggaggacccgactcttgtggtgctcacactgatgtacaatgtgggaatcctggaacaaaatgggaatcagggatttgtaactcagtgtttggaggattcaaaggaaatactaggaacctctcggttcttatcaacagacatttgaatgacagtttcaaatatttggtcatggcaagttatttcgattctgcagaggtaaatcgaatgggatttaacaaatatattcaaggatactctgataagctatgggataatggaaaagacattctcaagtatgttttaagccgtggaggatctgcagatcccaatttgaaggttactgcaatgcaaatgccaaattatcagggagaagttttatctatgagcgcctctcttgacatgatgaaacaattaatggaggatatgcgattggtgtacaagcatgcggctaacaagcatgtctctaatactcaacctgagcgtgaaagctacgatcctcctctacttcactacttggaagagagcttttttgaggactatactgaaagaatcagggatctagcagggaaattaaatatccttggaaaaattgcccggaatgacaagaccaaatac

Fragment Batch ID:

F51B1

Fragment Label ID:

51

Primer Group:

Ferr_LH_f_b634

Fragment Producer:

Christiane Trösse

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>78%

Phenotype

Number of Individuals (End):

23

Phenotype:

Most lice didn’t have egg strings at termination (but had first and second which had fallen off and were found in the filter). No reproduction.

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8a

8b

8c

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Average: 3.5 (1 vote)

8a: 5 of 7 lice have no or very short egg strings. 3 of 7 lice have very little gut content.

8b: 3 of 5 lice have no or very short egg strings. 3 of 5 lice have very little gut content.

8c: 8 of 11 lice have no or very short egg strings. 4 of 11 lice have very little gut content.

RNA-B EcR knockdown

Submitted by Anonymous (not verified) on 25 April, 2013 - 09:12

Phenotype CV:

General

Experiment Batch ID:

RNAB

Start Date:

19 March, 2012 - 09:06

End Date:

20 April, 2012 - 00:00

Experiment Description:

Knockdown of EcR. CPY185 used as control

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult

Fragment description

Fragment length:

667bp

Fragment concentration:

600ng/ul

Fragment Label ID:

18

Primer Group:

Validation

Efficacy of Knock Down:

F>20%

Phenotype

Number of Individuals (End):

14

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F18 treated lice

F18 treated lice close up

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Sampled lice had no eggstrings except one whioch most likely have not been injected properly.

H F60

Submitted by Sussie Dalvin on 9 April, 2013 - 13:26

Phenotype CV:

General

Experiment Batch ID:

H

Experiment Contact:

Sussie Dalvin

Start Date:

15 January, 2013 - 13:17

End Date:

25 February, 2013 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult female

Target description

Primary target ID:

EMLSAG00000003343, EMLSAG00000003343-686109 (gene) Lepeophtheirus salmonis

Gene or target symbol:

FK506

Fragment description

Fragment length:

542bp

Fragment concentration:

600ng/ul

Sense sequence:

ccacgggcccaggaactgttcatttgacaggttatttgattcccgacactgacgacatgattgacgacctcgattcagagtctgaatgtttnnnnnnnnnnnnnnnnnnagctcctgtccttgttaatggaaagggcaagaacaccaaacgtaagcctgataatagcttaattgatactcctaataaaaaagctaaaaaagagttggaacttgctagagctaaaaaagttcagtccgatcttaacttgaagaagaagagtcaaaagaaagtcgagtcttcggatgatgataacgatatggaagcagatgatgatacgtcggatgatggaatattagatttcactgagaattacgctgaagaagaggactctgtcgatgaatctgatgacgaatacgaggatgattcttcagtagaagaaggcgattcctctccaacaaagaaaattctcgagaaaatagccaataaaaaggagtcttctctcactactcccaagaacaataagaaaaaggataatgcctccactcctaaaggcgctactccc

Fragment Label ID:

F60

Primer Group:

FK506_f_b1313

Validation

Efficacy of Knock Down:

F>14%

Phenotype

Number of Individuals (End):

13

Phenotype:

no reproduction

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Females looked normal, but none had produced any eggstrings. Some animals had small empty eggstrings hanging behind them. Some eggs appeared vitellogenised but thin. Digestion looked normal.

Little pathology on RNAi animals sections. Eggs not vitellogenized

Uncertain about number of animals at endpoint

E F42

Submitted by Heidi Kongshaug on 12 March, 2013 - 10:38

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Frank Nilsen

Start Date:

15 October, 2012 - 10:19

End Date:

26 November, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g13677_Rheb

Fragment description

Fragment length:

460bp

Fragment concentration:

600ng/ul

Sense sequence:

atggggcctcccaagtctcgtaaaattgccttaatgggagctgtttccgtgggcaaatcttctctggccatccaatttgtacaaggacaatttgtcgattcctacgaaccaacaatcgaaaatacatttaccaaaacagttaaaattaatagacaagaatatgaaatttgtcttgttgatacagcaggccaaaatgaatactctatctttccattggaatattctgtaggaatagatggatatgttcttgtatactccattgattcacctaaaagcttagaagtttgtcaaatcatacaggaaaaattaatagaattcacaggaaacccaaatgttcccattgtcttggttggcaataaaaatgatttgtatatggaaagatatgtttcctttgaagaaggcaaaagaagagctgaagaaatgaaagcagtgtttcttgagacctctgccaaggaaaacc

Fragment Label ID:

F42

Primer Group:

Rheb_f_b1063

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>60%

Phenotype

Number of Individuals (End):

25

Phenotype:

Low reproduction and most are missing blood in the gut

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img_0128.jpg

img_0129.jpg

img_0131.jpg

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Eggestrenger:

12.11 F42 ser ut som der ikke er noen sunne eggstrenge mer (har vært tidligere).

15.11 Lite blod i F42.

E F34

Submitted by Christiane Trösse on 12 March, 2013 - 09:05

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Sussie Dalvin

Start Date:

15 October, 2012 - 00:04

End Date:

26 November, 2012 - 00:00

Experiment Description:

24 females recovered (62%), 7 with normal looking eggstrings, that hatch. Animals with eggstrings have blood in the gut, animals w/o eggstrings have little or no blood. Theis group of animals looked almost normal and was reproducing until 3 days before termination (last check). Seem to have been caught just when the system broke down. Reproducing animals had normally developed cops (morph and numbers)

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000003242, EMLSAG00000003242-686008 (gene) Lepeophtheirus salmonis

Gene or target symbol:

g7499_LsRaptor

Fragment description

Fragment length:

451bp

Fragment concentration:

600ng/ul

Sense sequence:

tcaagattcctcctcaactgccgctactccttggagactccttgaacgaatgaaaactgtgagtgttgctcttgtggtatgcctcaatgtcggggtggatcctccggatgtgacgaaaacctccccttgtgcttgtattgaggcatggattgatccaactcttctcaatccaacaagagcacttaatctcattggatctgctcttctaagacaatatgaacgttggcaacccagggctaggtatagacaatctttggacccaactgtggacgaagtccgacgcctagctatggctctacgtaaaaacgctagggatgaaagagttctttttcattataatggtcatggggtcccgaaaccaacggcaaacggagagatttgggtttttaatcggaattacactcagtatattcccctgagtctctacgatcttcaaacatggatgggctcc

Fragment Label ID:

F34

Primer Group:

Raptor_f_b918

Fragment Producer:

Heidi

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

24

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2a_f34.jpg

2b_f34.jpg

2c_f34.jpg

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Adult females recovered at termination of RNAi experiment. Experiment E.

E F50 (Ferr_sub)

Submitted by Christiane Trösse on 21 February, 2013 - 11:54

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Christiane Trösse

Start Date:

15 October, 2012 - 00:00

End Date:

26 November, 2012 - 00:00

Experiment Description:

Ferr:_sub

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

38

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000010792, EMLSAG00000010792-693558 (gene) Lepeophtheirus salmonis

Gene or target symbol:

ferr_sub

Fragment description

Fragment length:

568bp

Fragment concentration:

600ng/ul

Sense sequence:

acaggagcccatggatcttgtaacataggttcacagacggacccaaaaggaaaagcaactcctgaagatccaacaaattatcatgaattttgtatttcttcattaaggaaccaagtcaaaatggaatttgaagcagctcttcaatatctagttatgggagcctactttgatcaagacactatcaatctcaagggatttggtaagttcttttgggaaagtgctgacgaagaaagacagcatggattacaatttatcaaatacttgcgacacagaggagacaagggtctcgatttctttcaaggagatattgagcctattctaagaaagtattcatggagtgatggacttgatgctcttcgtgacgctcttatgatggaaaaaaaagtttcgggttccataaagaagatcatagatatttgcagtgttgaaggaaagcaagattatctctctggagattggctcactggtacatggttggaagagcaaatggatggacaaagaaagttagcaggacttatcaacacgttaagcaactttagaattgaccatgaggctcttggtgactgga

Fragment Batch ID:

F50B1

Fragment Label ID:

50

Primer Group:

Ferr_sub_f_b632

Fragment Producer:

Christiane Trösse

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>85%

Phenotype

Number of Individuals (End):

18

Phenotype:

Most lice didn’t have egg strings at termination (but had first and second which had fallen off and were found in the filter). No reproduction.

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7b

7c

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Average: 3.5 (1 vote)

7a: 6 of 9 lice have no or very short egg strings. 5 of 9 lice have very little gut content.

7b: 2 of 4 lice have no or very short egg strings. 2 of 4 lice have very little gut content.

7c: 3 of 5 lice have no or very short egg strings. 5 of 5 lice have very little gut content.