no eggstrings

AC F0S

Phenotype CV: 

General

Experiment Batch ID: 

AC

Experiment Contact: 

Heidi Kongshaug

Start Date: 

14 December, 2015 - 09:53

End Date: 

25 January, 2016 - 09:53
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

31

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

Gene or target symbol: 

CPY short version
Fragment description

Fragment length: 

320bp

Fragment concentration: 

600ng/ul

Sense sequence: 

cgttctgctcctcggagctgtcttcgctgaggaggacaagatcgtcggagggtatgagtgtacgaggcactcccaggcccaccaggtgtctctgaactctggataccacttctgtggaggctccctggtcagcaaggactgggtggtgtctgctgctcactgctacaagtcccgtattgaggtgcgtctgggcgagcaccacatcagggtcaacgagggaaccgagcagttcatctcctcctccagcgtcatccgtcaccccaactacagctcctacaacatcgacaacgacatcatgctgatcaagctgagcaagcccg

Fragment Label ID: 

F0

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

21
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Testing new and  shorter fragment of CPY

New fragment is 320 bp and called F0S

C F4 (CopB2)

Phenotype CV: 

General

Experiment Batch ID: 

C

Experiment Contact: 

Christiane Trösse

Start Date: 

14 August, 2012 - 09:00

End Date: 

24 September, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>82%
Phenotype

Number of Individuals (End): 

0
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Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F4 (CopB2)

Phenotype CV: 

General

Experiment Batch ID: 

A

Experiment Contact: 

Christiane Trösse

Start Date: 

23 January, 2012 - 09:00

End Date: 

24 February, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>65%
Phenotype

Number of Individuals (End): 

5
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Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

D F39

Phenotype CV: 

General

Experiment Batch ID: 

D

Experiment Contact: 

Heidi Kongshaug

Start Date: 

24 August, 2012 - 10:39
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

Gene or target symbol: 

EcR

Target type: 

consensus_mRNA
Fragment description

Fragment length: 

820bp

Fragment concentration: 

600ng/ul

Fragment ID: 

Fragment Label ID: 

F39

Primer Group: 

b1025 LsEcR Frag2 F
Validation

Efficacy of Knock Down: 

F>0%
Phenotype

Number of Individuals (End): 

16

Phenotype: 

No eggstring production
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No production of eggstrings Knockdown was not significant after 40 days

E F51 (Ferr_LH)

Phenotype CV: 

General

Experiment Batch ID: 

E

Experiment Contact: 

Christiane Trösse

Start Date: 

15 October, 2012 - 00:00

End Date: 

26 November, 2012 - 00:00

Experiment Description: 

<p>Ferr:_LH</p>
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

ferr_LH
Fragment description

Fragment length: 

676bp

Fragment concentration: 

600ng/ul

Sense sequence: 

<p>ctatttattgacaggctcaacgccgtgctttaagatgatcaaacaattattgcttttgtcaattttagttgcaagctgtgtgggaggacccgactcttgtggtgctcacactgatgtacaatgtgggaatcctggaacaaaatgggaatcagggatttgtaactcagtgtttggaggattcaaaggaaatactaggaacctctcggttcttatcaacagacatttgaatgacagtttcaaatatttggtcatggcaagttatttcgattctgcagaggtaaatcgaatgggatttaacaaatatattcaaggatactctgataagctatgggataatggaaaagacattctcaagtatgttttaagccgtggaggatctgcagatcccaatttgaaggttactgcaatgcaaatgccaaattatcagggagaagttttatctatgagcgcctctcttgacatgatgaaacaattaatggaggatatgcgattggtgtacaagcatgcggctaacaagcatgtctctaatactcaacctgagcgtgaaagctacgatcctcctctacttcactacttggaagagagcttttttgaggactatactgaaagaatcagggatctagcagggaaattaaatatccttggaaaaattgcccggaatgacaagaccaaatac</p>

Fragment Batch ID: 

F51B1

Fragment Label ID: 

51

Primer Group: 

Ferr_LH_f_b634

Fragment Producer: 

Christiane Trösse

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>78%
Phenotype

Number of Individuals (End): 

23

Phenotype: 

Most lice didn’t have egg strings at termination (but had first and second which had fallen off and were found in the filter). No reproduction.
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3.5
Average: 3.5 (1 vote)

8a: 5 of 7 lice have no or very short egg strings. 3 of 7 lice have very little gut content.

8b: 3 of 5 lice have no or very short egg strings. 3 of 5 lice have very little gut content.

8c: 8 of 11 lice have no or very short egg strings. 4 of 11 lice have very little gut content.

RNA-B EcR knockdown

Phenotype CV: 

General

Experiment Batch ID: 

RNAB

Start Date: 

19 March, 2012 - 09:06

End Date: 

20 April, 2012 - 00:00

Experiment Description: 

<p>Knockdown of EcR. CPY185 used as control</p>
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult
Fragment description

Fragment length: 

667bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

18

Primer Group: 

Validation

Efficacy of Knock Down: 

F>20%
Phenotype

Number of Individuals (End): 

14
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Sampled lice had no eggstrings except one whioch most likely have not been injected properly.

 

 

H F60

Phenotype CV: 

General

Experiment Batch ID: 

H

Experiment Contact: 

Sussie Dalvin

Start Date: 

15 January, 2013 - 13:17

End Date: 

25 February, 2013 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult female
Target description
Fragment description

Fragment length: 

542bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ccacgggcccaggaactgttcatttgacaggttatttgattcccgacactgacgacatgattgacgacctcgattcagagtctgaatgtttnnnnnnnnnnnnnnnnnnagctcctgtccttgttaatggaaagggcaagaacaccaaacgtaagcctgataatagcttaattgatactcctaataaaaaagctaaaaaagagttggaacttgctagagctaaaaaagttcagtccgatcttaacttgaagaagaagagtcaaaagaaagtcgagtcttcggatgatgataacgatatggaagcagatgatgatacgtcggatgatggaatattagatttcactgagaattacgctgaagaagaggactctgtcgatgaatctgatgacgaatacgaggatgattcttcagtagaagaaggcgattcctctccaacaaagaaaattctcgagaaaatagccaataaaaaggagtcttctctcactactcccaagaacaataagaaaaaggataatgcctccactcctaaaggcgctactccc

Fragment Label ID: 

F60

Primer Group: 

FK506_f_b1313
Validation

Efficacy of Knock Down: 

F>14%
Phenotype

Number of Individuals (End): 

13

Phenotype: 

no reproduction
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Females looked normal, but none had produced any eggstrings. Some animals had small empty eggstrings hanging behind them. Some eggs appeared vitellogenised but thin.  Digestion looked normal.

 

Little pathology on RNAi animals sections. Eggs not vitellogenized

Uncertain about number of animals at endpoint

E F42

Phenotype CV: 

General

Experiment Batch ID: 

E

Experiment Contact: 

Frank Nilsen

Start Date: 

15 October, 2012 - 10:19

End Date: 

26 November, 2012 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

g13677_Rheb
Fragment description

Fragment length: 

460bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atggggcctcccaagtctcgtaaaattgccttaatgggagctgtttccgtgggcaaatcttctctggccatccaatttgtacaaggacaatttgtcgattcctacgaaccaacaatcgaaaatacatttaccaaaacagttaaaattaatagacaagaatatgaaatttgtcttgttgatacagcaggccaaaatgaatactctatctttccattggaatattctgtaggaatagatggatatgttcttgtatactccattgattcacctaaaagcttagaagtttgtcaaatcatacaggaaaaattaatagaattcacaggaaacccaaatgttcccattgtcttggttggcaataaaaatgatttgtatatggaaagatatgtttcctttgaagaaggcaaaagaagagctgaagaaatgaaagcagtgtttcttgagacctctgccaaggaaaacc

Fragment Label ID: 

F42

Primer Group: 

Rheb_f_b1063

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>60%
Phenotype

Number of Individuals (End): 

25

Phenotype: 

Low reproduction and most are missing blood in the gut
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Eggestrenger:

12.11 F42 ser ut som der ikke er noen sunne eggstrenge mer (har vært tidligere).

15.11 Lite blod i F42.

E F34

Phenotype CV: 

General

Experiment Batch ID: 

E

Experiment Contact: 

Sussie Dalvin

Start Date: 

15 October, 2012 - 00:04

End Date: 

26 November, 2012 - 00:00

Experiment Description: 

<p>24 females recovered (62%), 7 with normal looking eggstrings, that hatch. Animals with eggstrings have blood in the gut, animals w/o eggstrings have little or no blood. Theis group of animals looked almost normal and was reproducing until 3 days before termination (last check). Seem to have been caught just when the system broke down. Reproducing animals had normally developed cops (morph and numbers)</p>
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

g7499_LsRaptor
Fragment description

Fragment length: 

451bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tcaagattcctcctcaactgccgctactccttggagactccttgaacgaatgaaaactgtgagtgttgctcttgtggtatgcctcaatgtcggggtggatcctccggatgtgacgaaaacctccccttgtgcttgtattgaggcatggattgatccaactcttctcaatccaacaagagcacttaatctcattggatctgctcttctaagacaatatgaacgttggcaacccagggctaggtatagacaatctttggacccaactgtggacgaagtccgacgcctagctatggctctacgtaaaaacgctagggatgaaagagttctttttcattataatggtcatggggtcccgaaaccaacggcaaacggagagatttgggtttttaatcggaattacactcagtatattcccctgagtctctacgatcttcaaacatggatgggctcc

Fragment Label ID: 

F34

Primer Group: 

Raptor_f_b918

Fragment Producer: 

Heidi
Validation

Efficacy of Knock Down: 

F>50%
Phenotype

Number of Individuals (End): 

24
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Adult females recovered at termination of RNAi experiment. Experiment E.

E F50 (Ferr_sub)

Phenotype CV: 

General

Experiment Batch ID: 

E

Experiment Contact: 

Christiane Trösse

Start Date: 

15 October, 2012 - 00:00

End Date: 

26 November, 2012 - 00:00

Experiment Description: 

<p>Ferr:_sub</p>
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

38

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

ferr_sub
Fragment description

Fragment length: 

568bp

Fragment concentration: 

600ng/ul

Sense sequence: 

<p>acaggagcccatggatcttgtaacataggttcacagacggacccaaaaggaaaagcaactcctgaagatccaacaaattatcatgaattttgtatttcttcattaaggaaccaagtcaaaatggaatttgaagcagctcttcaatatctagttatgggagcctactttgatcaagacactatcaatctcaagggatttggtaagttcttttgggaaagtgctgacgaagaaagacagcatggattacaatttatcaaatacttgcgacacagaggagacaagggtctcgatttctttcaaggagatattgagcctattctaagaaagtattcatggagtgatggacttgatgctcttcgtgacgctcttatgatggaaaaaaaagtttcgggttccataaagaagatcatagatatttgcagtgttgaaggaaagcaagattatctctctggagattggctcactggtacatggttggaagagcaaatggatggacaaagaaagttagcaggacttatcaacacgttaagcaactttagaattgaccatgaggctcttggtgactgga</p>

Fragment Batch ID: 

F50B1

Fragment Label ID: 

50

Primer Group: 

Ferr_sub_f_b632

Fragment Producer: 

Christiane Trösse

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>85%
Phenotype

Number of Individuals (End): 

18

Phenotype: 

Most lice didn’t have egg strings at termination (but had first and second which had fallen off and were found in the filter). No reproduction.
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3.5
Average: 3.5 (1 vote)

7a: 6 of 9 lice have no or very short egg strings. 5 of 9 lice have very little gut content.

7b: 2 of 4 lice have no or very short egg strings. 2 of 4 lice have very little gut content.

7c: 3 of 5 lice have no or very short egg strings. 5 of 5 lice have very little gut content.

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