RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
21 A F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39/5 Lepeophtheirus salmonis F>65.00% 29 April, 2015 - 15:44
RNA interference mediated knockdown of the KDEL receptor and...
22 AF F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:33
23 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
24 AF F228 EMLSAG00000006953, EMLSAG00000006953-689719 (gene) Lepeophtheirus salmonis
6953
PreAdult2 30/15 Lepeophtheirus salmonis 16 July, 2016 - 11:45
25 AF F239 (F233+F234) EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis, EMLSAT00000002881, EMLSAT00000002881-698728 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010949, EMLSAG00000002881
Adult, Adult 30/25 Lepeophtheirus salmonis 12 April, 2016 - 11:21
26 AF F241 EMLSAG00000002443, EMLSAG00000002443-685209 (gene) Lepeophtheirus salmonis
T2_2443
PreAdult2, Adult no visible phenotype 30/21 Lepeophtheirus salmonis 16 July, 2016 - 12:00
27 AF F263 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis
441, 12943
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:51
28 AF F264 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000006733, EMLSAG00000006733-689499 (gene) Lepeophtheirus salmonis
441, 12943, 6733
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:56
29 AG F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/15 Lepeophtheirus salmonis 22 March, 2018 - 14:40
30 AG F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No phenotype
no visible phenotype
30/16 Lepeophtheirus salmonis F>99.90% 21 February, 2017 - 10:15

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