RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
311 AX F418 EMLSAT00000010925, EMLSAT00000010925-706772 (mRNA) Lepeophtheirus salmonis
Trypsin- Dylan 		PreAdult2 survival, reduced 30/3 Lepeophtheirus salmonis 5 August, 2019 - 13:49
312 AX F262 EMLSAG00000006928, EMLSAG00000006928-689694 (gene) Lepeophtheirus salmonis
T19_6928 		PreAdult2 normal 30/14 Lepeophtheirus salmonis 5 August, 2019 - 13:39
313 AX F260 EMLSAT00000011145, EMLSAT00000011145-706992 (mRNA) Lepeophtheirus salmonis
T16_11145 		PreAdult2 normal 30/21 Lepeophtheirus salmonis 5 August, 2019 - 13:42
314 AX F259 EMLSAG00000012823, EMLSAG00000012823-695589 (gene) Lepeophtheirus salmonis
T6_12823 		PreAdult2 normal 30/13 Lepeophtheirus salmonis 5 August, 2019 - 13:45
315 AX F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2 normal 30/22 Lepeophtheirus salmonis 5 August, 2019 - 10:18
316 AV F404 EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult reproduction, female reproduction, egg string deformation, no eggstrings 30/15 Lepeophtheirus salmonis F>50.00% 2 May, 2019 - 09:49
317 AV F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3 		PreAdult2 normal
no visible phenotype 		30/15 Lepeophtheirus salmonis 2 May, 2019 - 09:38
318 AV F353 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype 		30/11 Lepeophtheirus salmonis 3 May, 2019 - 12:33
319 AV F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_2 		PreAdult2 normal
no visible phenotype 		30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:36
320 AV F352 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype 		30/11 Lepeophtheirus salmonis 2 May, 2019 - 11:24

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