D F31

Submitted by Heidi Kongshaug on 4 February, 2015 - 13:36

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:31

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

TOR

Fragment description

Fragment length:

505bp

Fragment concentration:

600ng/ul

Sense sequence:

tgtctggggactcagcctccaagatgggaggtatcctggccatagttgctctactcaacgcggatgtttgtaacacggggagtcgaattcatcgatttggaaattatcttcaaaacaattgtttaccgggagggaactccgtgacggatcctgtggtgattgccctggctactaaagccattggccgacttactcaagtatatggtagctacaaggctaatttacgctccgacttcattaattcactcgtcaagaaatcatttgagtggctttccaaccccaatgatcgcaatgatctcaagtggtacgctgctgttctcattctaagagaaatagccatttgtgagcccactttctttttccagaacgtaccaacgttttttgatcttatcttcattcctgtcagagactcaaaaccatttcttagagaatcagccgttaatgcgctacgagctggtctcatcgttactgcccaaagagaaaccaccaaacaatccagacgcca

Fragment Label ID:

F31

Primer Group:

LsTOR_f_b807

Validation

Efficacy of Knock Down:

F>0%

Phenotype

Number of Individuals (End):

14

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TOR, short eggstrings (14763), stunted growth of genital segment (90% of control), 2 ødipus eggstrings TOR: Significant downregulation (but only with primers outside the dsRNA fragments, both tested) NB! No downregulation BUT upregulation -95%

D F39

Submitted by Liv Sandlund on 12 December, 2014 - 10:54

Phenotype CV:

General

Experiment Batch ID:

D

Experiment Contact:

Heidi Kongshaug

Start Date:

24 August, 2012 - 10:39

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis

Gene or target symbol:

EcR

Target type:

consensus_mRNA

Fragment description

Fragment length:

820bp

Fragment concentration:

600ng/ul

Fragment ID:

EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis

Fragment Label ID:

F39

Primer Group:

b1025 LsEcR Frag2 F

Validation

Efficacy of Knock Down:

F>0%

Phenotype

Number of Individuals (End):

16

Phenotype:

No eggstring production

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No production of eggstrings Knockdown was not significant after 40 days

U F52

Submitted by Heidi Kongshaug on 8 December, 2014 - 13:56

Phenotype CV:

General

Experiment Batch ID:

U

Experiment Contact:

Sussie Dalvin

Start Date:

3 December, 2014 - 13:54

End Date:

14 January, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAG00000010179, EMLSAG00000010179-692945 (gene) Lepeophtheirus salmonis

Gene or target symbol:

g4675

Fragment description

Fragment length:

494bp

Fragment concentration:

600ng/ul

Sense sequence:

cgagtgacacaacgccgcaagaagtccaaaccactacgactaaagcgatgtcttccacggagcaagaacctacaacgacgactctactttctgactccaaagaatcatctgagcccaaaaaagaactcacaaagaaaatgagcataaaaaaggaccaagaacatacaattgattcaataattccatgtactgatatagatgaggacgcttgtcaatcttttaaaccactttgtaaaatcgaaatcgaaataggatttcgtcgttgtcaaaaaacatgcggtttttgcaaggggaagagacttatttatgaggccaaaatttcaaacggtgttgaaggacttgtagaatttcaacaattttatccgagacagcctgtatatatggctggttgttttgaaaaatctggtataaaaaacacaattactccacacgcttttgttattcatgaaagaagtgaagaaccttcaatgaaggattccaaatgcccacatgca

Fragment Label ID:

F52

Primer Group:

g4675_f_b1230

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

10

Phenotype:

Non-hatching eggstrings

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This experiment was performed by injection of males only in the normal set-up, i.e. young adult males and placed on fish together with non-injected females. Only males were sampled at termination, but females were photographed and eggstrings put for hatching (photos named as the hatching well).

T F166

Submitted by Heidi Kongshaug on 14 November, 2014 - 13:26

Phenotype CV:

General

Experiment Batch ID:

T

Experiment Contact:

Frank Nilsen

Start Date:

30 October, 2014 - 13:20

End Date:

9 December, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000010071, EMLSAT00000010071-705918 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

UN50_10071

Fragment description

Fragment length:

457bp

Fragment concentration:

600ng/ul

Sense sequence:

cgaatccgcagatgaaggagtctatgacttctgggtgtctccacgtgtgggctcttatgctaaggtaatggttgctcctggatacgtttctacattcgaacaattcttgaaggagcaayccattgcctctactcttttgattgaggatgtggaaattactaatcaaagattttccatcattgaaaatgctttaagaaaaatcaaaacttcatccagatcaattgactttgaaaactactacagttcagacgatcttraagccgataatggctttgtcgaagttgaaacctatggaaaatcctacgaaggtagagacttaaaggtcatcaagattatgaaggctggacctaatgctcccaatatcttcattgaggctggaattcatgcacaagatcctgaaaatgctcgttaccttgaccaattcaatttccacatcatgccttccgttaaccccgat

Fragment Label ID:

F166

Primer Group:

likb2496_T7_b2546

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>96%

Phenotype

Number of Individuals (End):

19

Phenotype:

Abnormal

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Short and malformed eggstrings, no mortality

R F161

Submitted by Heidi Kongshaug on 7 October, 2014 - 10:17

Phenotype CV:

General

Experiment Batch ID:

RNAi R

Experiment Contact:

Frank Nilsen

Start Date:

21 August, 2014 - 10:10

End Date:

1 October, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

37

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000580, EMLSAG00000000580-683346 (gene) Lepeophtheirus salmonis

Gene or target symbol:

UN38_580

Fragment description

Fragment length:

472bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F

Primer Group:

b2527

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

20

Phenotype:

Abnormal eggstrings in some individuals

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The experiment was performed to check the phenotype of the gene. Abnormal eggstringproduction was observed at termination. Normal looking copepods developed from the eggstrings. Normal Activity. Sample RFR2 was sent for sectioning, abnormal

P F120

Submitted by Christiane Trösse on 11 March, 2014 - 16:23

Phenotype CV:

General

Experiment Batch ID:

P

Experiment Contact:

Frank Nilsen

Start Date:

7 March, 2014 - 15:55

End Date:

22 April, 2014 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000693, EMLSAG00000000693-683459 (gene) Lepeophtheirus salmonis

Gene or target symbol:

UN28_g13803

Fragment description

Fragment length:

510bp

Fragment concentration:

600ng/ul

Sense sequence:

tgctctggaagatgatgaacacgatattacttttgctccgcccaattctccgattgtaccttccaatgttgttatgagaggcactatcaacattccttctcttgatcagactttgaatgcagaagacccagaaggagcatcccccattgttagaggagtttttgattcaacttggtttccatattccaactacgagaattgtccagttacccctgacgcaaaccaaacaacgtatggaagtgtatatccacacttatcttaccggcatgatctcacgtttcgagatcttcaacttgcttcctcttttcctaataatagcaaccaatttctgaatgaaagtaatatggaaattctacacaattctcttctagaatctaaatggaaacccaaggacaaaatatttgtggacctcaacaatacacttgatgaaaaagataatatcgactataactcaactattcttgcgaacaataataataacaacagtagtgtccttgagcccatgttcca

Fragment Label ID:

F120

Primer Group:

UN28_g13803_f_b1875

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>5%

Phenotype

Number of Individuals (End):

13

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3 individuals had very special curly eggstrings , 3 had no eggstrings , low reproduction

L F98

Submitted by Heidi Kongshaug on 22 January, 2014 - 10:44

Phenotype CV:

female reproduction

General

Experiment Batch ID:

L

Experiment Contact:

Sussie Dalvin

Start Date:

20 March, 2013 - 14:29

End Date:

26 April, 2013 - 12:43

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult

Adult

Target description

Primary target ID:

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis

Gene or target symbol:

Kiran

Fragment description

Fragment length:

240bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F98

Primer Group:

b1642

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Phenotype:

One animal for sectioning

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Ac1_g24758 A group in experiment L. hjhjk hkhkj hjhkh hhkjh hkhkjh hkhkh

E F51 (Ferr_LH)

Submitted by Christiane Trösse on 28 June, 2013 - 11:43

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Christiane Trösse

Start Date:

15 October, 2012 - 00:00

End Date:

26 November, 2012 - 00:00

Experiment Description:

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000006305, EMLSAT00000006305-702152 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

ferr_LH

Fragment description

Fragment length:

676bp

Fragment concentration:

600ng/ul

Sense sequence:

<p>ctatttattgacaggctcaacgccgtgctttaagatgatcaaacaattattgcttttgtcaattttagttgcaagctgtgtgggaggacccgactcttgtggtgctcacactgatgtacaatgtgggaatcctggaacaaaatgggaatcagggatttgtaactcagtgtttggaggattcaaaggaaatactaggaacctctcggttcttatcaacagacatttgaatgacagtttcaaatatttggtcatggcaagttatttcgattctgcagaggtaaatcgaatgggatttaacaaatatattcaaggatactctgataagctatgggataatggaaaagacattctcaagtatgttttaagccgtggaggatctgcagatcccaatttgaaggttactgcaatgcaaatgccaaattatcagggagaagttttatctatgagcgcctctcttgacatgatgaaacaattaatggaggatatgcgattggtgtacaagcatgcggctaacaagcatgtctctaatactcaacctgagcgtgaaagctacgatcctcctctacttcactacttggaagagagcttttttgaggactatactgaaagaatcagggatctagcagggaaattaaatatccttggaaaaattgcccggaatgacaagaccaaatac</p>

Fragment Batch ID:

F51B1

Fragment Label ID:

51

Primer Group:

Ferr_LH_f_b634

Fragment Producer:

Christiane Trösse

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>78%

Phenotype

Number of Individuals (End):

23

Phenotype:

Most lice didn’t have egg strings at termination (but had first and second which had fallen off and were found in the filter). No reproduction.

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8a

8b

8c

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Average: 3.5 (1 vote)

8a: 5 of 7 lice have no or very short egg strings. 3 of 7 lice have very little gut content.

8b: 3 of 5 lice have no or very short egg strings. 3 of 5 lice have very little gut content.

8c: 8 of 11 lice have no or very short egg strings. 4 of 11 lice have very little gut content.

L F102

Submitted by Heidi Kongshaug on 6 June, 2013 - 10:00

Phenotype CV:

General

Experiment Batch ID:

L F102

Experiment Contact:

Sussie Dalvin

Start Date:

26 April, 2013 - 09:57

End Date:

10 June, 2013 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

29

Developmental Stage CV:

PreAdult

Adult

Target description

Primary target ID:

EMLSAT00000004662, EMLSAT00000004662-700509 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

UN21_g1627

Fragment description

Fragment length:

443bp

Fragment concentration:

600ng/ul

Sense sequence:

Tccgcttttttatctggaatatggcttatgtacgaagacatgaactacaatcaaggaaatccaggaggmaaagtagtttacggatggggatataatcatagcattcattataatattgacataagcaagatttcctctatgcgctacagtggaacagaatctggattacaatttgatactttgaacttttacaaagagccaggattcataggaaacgaggagatgtactatcaagacgaagcaatattgactcgacccttctttggaaaatccattattctgacaggatgcaaaccgtggactttatttaatgaaactcattttcatggtgaacatatttgtatctatccaagatctatatcatcaaataattgtactcctggtttttttgaagctccaagggattttggtgactttgtagataaggtgaggagcattcggct

Fragment Label ID:

F102

Primer Group:

b1629

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

4

Phenotype:

One animal sectioned

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Few animals, one empty tank (7c) and a total of 4 animal recovered

Ovaries abnormal, eggs in oviduct abnormal. Subcuticular tissue normal.

Glandular tissue appears large ? or somehow there is less of everything else, unsure.

inconclusive in-situ

The student Julia did the qPCR validation of the sampled lice. 130927

L F97

Submitted by Sussie Dalvin on 7 May, 2013 - 10:13

Phenotype CV:

female reproduction

General

Experiment Batch ID:

L

Experiment Contact:

Sussie Dalvin

Start Date:

17 April, 2013 - 14:29

End Date:

10 June, 2013 - 12:43

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

29

Developmental Stage CV:

PreAdult

Adult

Target description

Primary target ID:

EMLSAG00000002641, EMLSAG00000002641-685407 (gene) Lepeophtheirus salmonis

Fragment description

Fragment length:

485bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F97

Primer Group:

b1301

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

7

Phenotype:

One animal for sectioning

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A group in experiment L. The animals have problems with reproduction: a total of 7 animals were recovered, 4 didnt have anystrings, the other 3 had short to very short eggstrings. Digestion appeared normal.

QPCR using two sybr green probes outside target sequence did not show significant downregulation of the gene (n=5) Julia Stinn performed QPCR. Assays were titrated in 6 (?) dilutions.