AL Hormone receptor 3 (HR3)

Submitted by Joakim Brunet on 14 June, 2018 - 10:47

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 10:00

Experiment Description:

Knockdown of the ortholog of the nuclear receptor HR3. Injection of pre-adult 2 females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000000733

Target type:

mRNA

Fragment description

Fragment length:

441bp

Sense sequence:

gccta Gcaccattcgagcaagaaatctcattgcaaaacaaacaaacaaaaaggattcatagaata Aatagaaaaaggaccctacaaggcaaaaaaaacagcaactattttttacaaattgtttcg Tactcgtcgatgcaaaatcagaaatattatacgaatttgtcctcaatggattggatatca Ggctatcgtcttccatccaattcggatttaaagtgtgatgatgatcaccatggcggcggg Gcaggagtacagcatgcatctgttgtcggcggaccaacaggcggagcactaggatctggc Tcttccggtcccctcacaaacaacaccaattccaactcttcctcaccctccatgctaggc Atgtgggcagcagctgtggccaatacacctcttaaagtggaaacagctgatcatcatgga Aatggatctctggtgg

Fragment Batch ID:

F341

Fragment Label ID:

F341

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

17

Phenotype:

No eggstrings. Disorganization of eggs in genital segment

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RFAL16_HR3_s109_s2_genital_2.5x_AX10_ScopeA1.jpeg

RAL72.jpg

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Knockdown of HR3 in pre-adult 2 females. Resulted in no eggstring production. Sections reveals disorganized eggs in genital segment. Ovaries look normal.

AQ F375 EMLSAG00000008959

Submitted by Christiane Eichner on 29 May, 2018 - 14:58

Phenotype CV:

General

Experiment Batch ID:

RAQ

Experiment Contact:

Christiane Eichner

Start Date:

16 April, 2018 - 09:00

End Date:

23 May, 2018 - 09:00

Experiment Description:

Knock-down of EMLSAG00000008959

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000008959

Fragment description

Fragment length:

432bp

Fragment concentration:

600ng/ul

Sense sequence:

gcctccggttcggatgaagaagtagaagaggaagaaataactgaatatgatgccttgttaaaggtgtatggagctcaaacatatgttgatgaagctgaagaggaagagatatgcgaaggagaaatggaggaggaagaaaacgatatggatgaggaagatgtatcattgaatggagtagatgggatagatgaagaagatttattgtcgaatgaagaagaaaatgaaactatcgatctcgaaagtggagaagatgaaaatgaaactatcgatctcgaaagtggagaagaggaaaatgaaactatcgatctcgaaagtggagaagaagaagaagagattgtggatactgatatcggaagkgrtatattagatgaaaatgaggatgaatttccagagatggatgacgaaaawgacacttgtggcccctctgatcct

Fragment Label ID:

F375

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

5

Phenotype:

no eggstreng

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8959_RAQ.jpg

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Knock-down of EMLSAT00000003179. No visible phenotype

AP F367 EMLSAG00000005299

Submitted by Christiane Eichner on 29 May, 2018 - 12:27

Phenotype CV:

General

Experiment Batch ID:

AP

Experiment Contact:

Christiane Eichner

Start Date:

6 March, 2018 - 09:00

End Date:

10 April, 2018 - 09:00

Experiment Description:

EMLSAG1458 knock-down

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000005299

Fragment description

Fragment length:

338bp

Fragment concentration:

600ng/ul

Sense sequence:

>F367 gtatgatgacggacatgctcaagggtaacattactaacatgttgccaatgattgtaattggaggatggatcaactggcatttctcaggctttgtcactaccaaaatcccttttcccctcactcttcgtttcaagcctatgcttcaaagaggcattgaactcatgagtctagatgcttcatgggtctcctccgcctcctggtactttcttaacgttttcggactacgatctatttatgacttagttctcggagaaaataatgttgcggatcaatcaagaatcatgcaggaccagatgatgatgcctgccaacggaatggctaccgactataaacaggcc

Fragment Label ID:

F367

Primer Group:

likb2502_T7_b2515

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Eichner

Validation

Efficacy of Knock Down:

F>16%

Phenotype

Number of Individuals (End):

24

Phenotype:

slightly shorter gonade and abdominal segment

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Testing the fragment for Mvp1 knock-down.

AP F366 EMLSAT00000001458

Submitted by Christiane Eichner on 29 May, 2018 - 11:26

Phenotype CV:

General

Experiment Batch ID:

AP

Experiment Contact:

Christiane Eichner

Start Date:

6 March, 2018 - 09:00

End Date:

10 April, 2018 - 09:00

Experiment Description:

EMLSAG1458 knock-down

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000001458

Fragment description

Fragment length:

412bp

Fragment concentration:

600ng/ul

Sense sequence:

>F366 caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Fragment Label ID:

F366

Primer Group:

likb2502_T7_b2515

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Eichner

Validation

Efficacy of Knock Down:

F>77%

Phenotype

Number of Individuals (End):

13

Phenotype:

no eggstreng

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Testing the fragment for Mvp1 knock-down.

Mucin-like spermatophore wall protein 2 (AJ F270)

Submitted by Andreas Borchel on 26 March, 2018 - 10:39

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adut males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP2

Fragment description

Fragment length:

497bp

Fragment concentration:

600ng/ul

Sense sequence:

CCAGGCTTTTCACACGCTTT

Antisense sequence:

GCTCTTGGCCGAGCATAAAA

Fragment Batch ID:

F270

Fragment Label ID:

F270

Validation

Efficacy of Knock Down:

F>80%

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Control fragment used for all samples

Mucin-like spermatophore wall protein 1 (AJ F269)

Submitted by Andreas Borchel on 26 March, 2018 - 10:34

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adult males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP1

Fragment description

Fragment length:

527bp

Fragment concentration:

600ng/ul

Sense sequence:

TTACGTTGGCAATCGTCGTC

Antisense sequence:

GAAATCAAGTGCGGCTCCA

Fragment Batch ID:

F269

Fragment Label ID:

F269

Validation

Efficacy of Knock Down:

F>95%

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Control fragment used for all samples

LsRXR

Submitted by Michael Dondrup on 11 April, 2016 - 12:18

Phenotype CV:

General

Experiment Batch ID:

LsRXR

Experiment Contact:

Christiane Eichner

Michael Dondrup

Frank Nilsen

Start Date:

13 March, 2015 - 11:54

End Date:

7 April, 2015 - 10:02

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

50

Developmental Stage CV:

Adult

Copepodid

Target description

Primary target ID:

EMLSAT00000001919, EMLSAT00000001919-697766 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsRXR

Target type:

protein_coding_primary_transcript

Fragment description

Fragment length:

301bp

Fragment Label ID:

RNAiFr1

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>60%

Phenotype

Number of Individuals (End):

38

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Background

AC F0S

Submitted by Heidi Kongshaug on 31 March, 2016 - 09:56

Phenotype CV:

General

Experiment Batch ID:

AC

Experiment Contact:

Heidi Kongshaug

Start Date:

14 December, 2015 - 09:53

End Date:

25 January, 2016 - 09:53

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

31

Developmental Stage CV:

Adult

Target description

Primary target ID:

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol:

CPY short version

Fragment description

Fragment length:

320bp

Fragment concentration:

600ng/ul

Sense sequence:

cgttctgctcctcggagctgtcttcgctgaggaggacaagatcgtcggagggtatgagtgtacgaggcactcccaggcccaccaggtgtctctgaactctggataccacttctgtggaggctccctggtcagcaaggactgggtggtgtctgctgctcactgctacaagtcccgtattgaggtgcgtctgggcgagcaccacatcagggtcaacgagggaaccgagcagttcatctcctcctccagcgtcatccgtcaccccaactacagctcctacaacatcgacaacgacatcatgctgatcaagctgagcaagcccg

Fragment Label ID:

F0

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

21

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RAC27.tif

RAC29.tif

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Testing new and shorter fragment of CPY

New fragment is 320 bp and called F0S

AA F222

Submitted by Anna Komisarczuk on 20 October, 2015 - 14:33

Phenotype CV:

General

Experiment Batch ID:

AA

Experiment Contact:

Anna Komisarczuk

Start Date:

8 September, 2015 - 14:29

End Date:

15 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Number of Individuals (Start):

120

Developmental Stage CV:

PreAdult1 female

PreAdult2 male

Target description

Primary target ID:

EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis

EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis

EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis

EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis

Fragment description

Fragment Label ID:

F222

Primer Group:

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No spermatophores on females. Specific? Mix of dsRNA against 4 genes.

Z F198

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:31

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000527, EMLSAG00000000527-683293 (gene) Lepeophtheirus salmonis

Gene or target symbol:

527

Fragment description

Fragment length:

415bp

Fragment concentration:

569ng/ul

Sense sequence:

cctcaggccccaaagctctggttattaaacttatttttgaataccaaggagagcgttctaatagtccaaaaataagaaagcttgtgctagaagaaggagagacgccgactctgggcttcttatgtgaatctttttctttaaaaagctcgcaaacattttattactacgatgatgacggggatcaaataactatatccaatgatcgagagcttctgacagccatcaaggaaaagaagtcctctgatacactaaggctctatacaggaacagagcttattccaggagatattccgagtaaaaggaaccatgagaaagatatttttgaagagatttgtaccaattgtcagaaccgaatcggtcgattctcttataaatgtgttatttgtagcggtgtgaagccttcggagtgcactcg

Fragment Label ID:

F198

Primer Group:

b3154

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

20

Phenotype:

slightly wavy ES-check hatching

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Unknown function of gene

Slightly wavy egg strings.

Downregulation not verified by qPCR.