Z F214

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:21

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000426, EMLSAG00000000426-683192 (gene) Lepeophtheirus salmonis

Gene or target symbol:

426

Fragment description

Fragment length:

766bp

Fragment concentration:

600ng/ul

Sense sequence:

agtgggatttgcttgcaggagtgtataaatttagggtttccacctttttaaaaagtcttgtatttgaatccaaagaaaaaagagtgaccattgatcgttctctaataaaagactatggaatcagatcaatcaataagcgaaaatattatttcttctgccttgggagtaggagcccctttggtaagggggaaattatccttaatagttttgattttgatttggatgaatttaacaattatgctctatcactccatgctaacaaaagtttaagcttaaaacgatcagtttctcgaaaaaatggaagaattaagtcaagaacaagttcagaatctgatgttaccaaacaagtttccaatttatccaaactgttttcaaaattgtttaagagcaaaaaagataggaagaaaaaattgttgaaggatagggattcgttgacatctaaagaggattactcagttgttgatgaatattcggaaatcttaccaagtggctatagtggtcaaacttatctctgcacttctgaagatttatcggatagtgaatctatgggkcttgtattkccttctccygattttwagacaaaatcctgcaaatcctttaaaaaaaataatcctaaaaaccatctcaaatggcatgaacagaatgaaatttactccgagtacgctccaagttatttgattcctgttgagcatgaaacaaaacatgaagttcttgtaaaagaaacaggagatgtctacaccctaccaatgacaaagaaaaagccaag

Fragment Label ID:

F214

Primer Group:

b3309

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

19

Phenotype:

Many bad and short eggstrings. Check hatching numbers

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Unknown function of gene.

Downregulation not verified by qPCR.

Z F204

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:12

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000106, EMLSAG00000000106-682872 (gene) Lepeophtheirus salmonis

Gene or target symbol:

106

Fragment description

Fragment length:

553bp

Fragment concentration:

324ng/ul

Sense sequence:

actcccagctgcagctgtgatatagtttcttctcatattaataaacaaggaactagtgctatcatccctactttatcctgtagcaatgaacgctttaaaatatcggatctctctcgcagaaatactagagagtactcttcttctcctcaatgtgatcaaggactcattcttttaaaacgtcttcgaatagcagataaagagctcaaagcccatgaatggatgagtgtgtgtgggaatcataaaaaatatttgcttcaggattttcctctcaaaaaccaccaatgcggtatttgttttaacagtgatcaaatacggcttagaagagtttctacaaagtcgcaggatttgtacaaaggagaagtcgtggttggggaattcgtatgttcggagtgtgatgatttgatattagatggagaaggggatgagagtcagcataaaaaaataaccattgggcagatttgggttacattggataattcattttgtcttattaatagctctgtaccttcgctcagattattatgtagaggcttaaccaacaaagagccccttc

Fragment Label ID:

F204

Primer Group:

b3242

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

20

Phenotype:

Slightly wavy ES-check hatching

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Unknown function of gene.

Slightly wavy egg strings.

Downregulation not verified by qPCR.

Z F220

Submitted by Heidi Kongshaug on 3 September, 2015 - 08:53

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Sussie Dalvin

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 10:33

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

TOR

Fragment description

Fragment length:

457bp

Fragment concentration:

600ng/ul

Sense sequence:

aacggccttggatccagcaatatttgcaaacatcagcatgttggctcgagcagtaaaaggctctgttaagaacgacgttgctcctatgttagactccatgtttagtgttggacttagtccatctcttactatggcacttcatgagttagcgaggcatattccgtctctcaagaaagaaattgctgatggacttctcaaaatattatcacttatattaatgcaacaaccgtttagacacccaggaactcctattgcttccgcagctaattctcctactcctcctatgactctcgaaccaattccatccactgcatctgttgttcttggactacgcactctcggaacttttgattttgaaggtcactcattacttcaatttgtcagacattgtgctgatacatacctctataaagttgagaaagtggtccgacttgaagccgtcaagacatgctcctct

Fragment Label ID:

F220

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

17

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Repeat injections of TOR. Take out samples at 10 and 20 days after injections + at end. Look at the varations.

15 days post injections: 70% downregulation

25 days post injections: 50 % downregulation

35 days post injections 20% downregulation

X F201 (ZIP13)

Submitted by Christiane Trösse on 27 July, 2015 - 14:33

Phenotype CV:

General

Experiment Batch ID:

X

Experiment Contact:

Christiane Trösse

Start Date:

17 March, 2015 - 13:44

End Date:

22 April, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000012537, EMLSAG00000012537-695303 (gene) Lepeophtheirus salmonis

Gene or target symbol:

ZIP13

Fragment description

Fragment length:

546bp

Fragment concentration:

600ng/ul

Fragment Label ID:

F201

Primer Group:

b3122

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Phenotype

Number of Individuals (End):

7

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RNAi with preadult females. 86% of recovered lice had missing or abnormal egg strings, but could be a coincidence (low lice numbers). No hatching data from IMR. qPCR validation not done yet. Fotos (RX28-32, RFX8-9) on P:\Sealice\RNAi\RNAi_fotos_samplet\Experiment_X\RNAiX).

C F4 (CopB2)

Submitted by Christiane Trösse on 24 July, 2015 - 16:11

Phenotype CV:

General

Experiment Batch ID:

C

Experiment Contact:

Christiane Trösse

Start Date:

14 August, 2012 - 09:00

End Date:

24 September, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>82%

Phenotype

Number of Individuals (End):

0

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Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

B F2+F3 (IRP1+IRP2)

Submitted by Christiane Trösse on 29 April, 2015 - 16:21

Phenotype CV:

General

Experiment Batch ID:

B

Experiment Contact:

Christiane Trösse

Start Date:

19 March, 2012 - 09:00

End Date:

18 April, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis

EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsIRP1B

LsIRP1A

Fragment description

Fragment length:

441bp

Fragment concentration:

600ng/ul

Sense sequence:

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID:

F3

F2

Fragment Mixture Formula:

1:1 mixture, resulting in 300 ng/ul for each of the fragments

Primer Group:

b640

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>44%

Phenotype

Number of Individuals (End):

23

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Knockdown of LsIRP1A+LsIRP1B (formerly called IRP1 and IRP2) in preadult female lice. Slightly shorter egg strings. Knockdown 62% for IRP1 and 44% for IRP2. Described in the fragment fields is only F3, see experiment A for F2.

A F4 (CopB2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:44

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>65%

Phenotype

Number of Individuals (End):

5

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Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F3 (IRP2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:16

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsIRP1B

Fragment description

Fragment length:

441bp

Fragment concentration:

600ng/ul

Sense sequence:

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID:

F3

Primer Group:

b640

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

23

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Knockdown of LsIRP1B (formerly called IRP2) in preadult female lice. Slightly shorter egg strings caused less offspring.

D F32/F33

Submitted by Heidi Kongshaug on 5 February, 2015 - 08:46

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:49

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis

EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g18363_TSC2

NLG3408_TSC1

Fragment description

Fragment concentration:

600ng/ul

Fragment Label ID:

F

Primer Group:

LS_TSC2-F_b872

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>30%

Phenotype

Number of Individuals (End):

14

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TSC1+2, short eggstrings (15853), not the best appetite jlkj jkljlkj jk kl lj l jklkj

D F33

Submitted by Heidi Kongshaug on 4 February, 2015 - 13:53

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:49

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g18363_TSC2

Fragment description

Fragment length:

432bp

Fragment concentration:

600ng/ul

Sense sequence:

aaaatgggcattcagcgacttggttgattggcacaatgcttataactgtttctacgagctgttgtacccatattgcaatgagaaatggtgtatgtgatagatgttatttttattgcattcgagatggaactttaagtaaggatgatattactaaaaattcggatagaagaagacacatgtcggatttttactccggtacaaataagagtcagttatcaaacttaagaagtgataaggaacttcataggcacacttctctggagagaatggagtcttctggagatgattatagagtaaaaaaagtgctacctgaaacatgcagttgttggtgctctggctgggctgaaatttacattcggcgtccaagtggggatgtatcctggatgtgtagagtgcaaaatactcagttgcttagtgcgcatacaccctcaa

Fragment Label ID:

F33

Primer Group:

LS_TSC2-F_b872

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>72%

Phenotype

Number of Individuals (End):

11

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Tsc2, a bit short eggstring TSC-2: Significant downregulation (but only with primers outside the dsRNA fragments, both tested)