female reproduction

Z F214

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Frank Nilsen

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

766bp

Fragment concentration: 

600ng/ul

Sense sequence: 

agtgggatttgcttgcaggagtgtataaatttagggtttccacctttttaaaaagtcttgtatttgaatccaaagaaaaaagagtgaccattgatcgttctctaataaaagactatggaatcagatcaatcaataagcgaaaatattatttcttctgccttgggagtaggagcccctttggtaagggggaaattatccttaatagttttgattttgatttggatgaatttaacaattatgctctatcactccatgctaacaaaagtttaagcttaaaacgatcagtttctcgaaaaaatggaagaattaagtcaagaacaagttcagaatctgatgttaccaaacaagtttccaatttatccaaactgttttcaaaattgtttaagagcaaaaaagataggaagaaaaaattgttgaaggatagggattcgttgacatctaaagaggattactcagttgttgatgaatattcggaaatcttaccaagtggctatagtggtcaaacttatctctgcacttctgaagatttatcggatagtgaatctatgggkcttgtattkccttctccygattttwagacaaaatcctgcaaatcctttaaaaaaaataatcctaaaaaccatctcaaatggcatgaacagaatgaaatttactccgagtacgctccaagttatttgattcctgttgagcatgaaacaaaacatgaagttcttgtaaaagaaacaggagatgtctacaccctaccaatgacaaagaaaaagccaag

Fragment Label ID: 

F214

Primer Group: 

b3309

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

19

Phenotype: 

Many bad and short eggstrings. Check hatching numbers
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Unknown function of gene.

Downregulation not verified by qPCR.

Z F204

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Frank Nilsen

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

553bp

Fragment concentration: 

324ng/ul

Sense sequence: 

actcccagctgcagctgtgatatagtttcttctcatattaataaacaaggaactagtgctatcatccctactttatcctgtagcaatgaacgctttaaaatatcggatctctctcgcagaaatactagagagtactcttcttctcctcaatgtgatcaaggactcattcttttaaaacgtcttcgaatagcagataaagagctcaaagcccatgaatggatgagtgtgtgtgggaatcataaaaaatatttgcttcaggattttcctctcaaaaaccaccaatgcggtatttgttttaacagtgatcaaatacggcttagaagagtttctacaaagtcgcaggatttgtacaaaggagaagtcgtggttggggaattcgtatgttcggagtgtgatgatttgatattagatggagaaggggatgagagtcagcataaaaaaataaccattgggcagatttgggttacattggataattcattttgtcttattaatagctctgtaccttcgctcagattattatgtagaggcttaaccaacaaagagccccttc

Fragment Label ID: 

F204

Primer Group: 

b3242

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

20

Phenotype: 

Slightly wavy ES-check hatching
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Unknown function of gene.

Slightly wavy egg strings.

Downregulation not verified by qPCR.

 

Z F220

Phenotype CV: 

General

Experiment Batch ID: 

Z

Experiment Contact: 

Sussie Dalvin

Start Date: 

27 August, 2015 - 13:44

End Date: 

1 October, 2015 - 10:33
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description
Fragment description

Fragment length: 

457bp

Fragment concentration: 

600ng/ul

Sense sequence: 

aacggccttggatccagcaatatttgcaaacatcagcatgttggctcgagcagtaaaaggctctgttaagaacgacgttgctcctatgttagactccatgtttagtgttggacttagtccatctcttactatggcacttcatgagttagcgaggcatattccgtctctcaagaaagaaattgctgatggacttctcaaaatattatcacttatattaatgcaacaaccgtttagacacccaggaactcctattgcttccgcagctaattctcctactcctcctatgactctcgaaccaattccatccactgcatctgttgttcttggactacgcactctcggaacttttgattttgaaggtcactcattacttcaatttgtcagacattgtgctgatacatacctctataaagttgagaaagtggtccgacttgaagccgtcaagacatgctcctct

Fragment Label ID: 

F220

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>50%
Phenotype

Number of Individuals (End): 

17
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Repeat injections of TOR. Take out samples at 10 and 20 days after injections + at end. Look at the varations.

15 days post injections: 70% downregulation

25 days post injections: 50 % downregulation

35 days post injections 20% downregulation

X F201 (ZIP13)

Phenotype CV: 

General

Experiment Batch ID: 

X

Experiment Contact: 

Christiane Trösse

Start Date: 

17 March, 2015 - 13:44

End Date: 

22 April, 2015 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

546bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F201

Primer Group: 

b3122

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Phenotype

Number of Individuals (End): 

7
Upload

Image Quality: 

0
No votes yet

RNAi with preadult females. 86% of recovered lice had missing or abnormal egg strings, but could be a coincidence (low lice numbers). No hatching data from IMR. qPCR validation not done yet. Fotos (RX28-32, RFX8-9) on P:\Sealice\RNAi\RNAi_fotos_samplet\Experiment_X\RNAiX).

C F4 (CopB2)

Phenotype CV: 

General

Experiment Batch ID: 

C

Experiment Contact: 

Christiane Trösse

Start Date: 

14 August, 2012 - 09:00

End Date: 

24 September, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>82%
Phenotype

Number of Individuals (End): 

0
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

B F2+F3 (IRP1+IRP2)

Phenotype CV: 

General

Experiment Batch ID: 

B

Experiment Contact: 

Christiane Trösse

Start Date: 

19 March, 2012 - 09:00

End Date: 

18 April, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

441bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID: 

F3
F2

Fragment Mixture Formula: 

1:1 mixture, resulting in 300 ng/ul for each of the fragments

Primer Group: 

b640

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>44%
Phenotype

Number of Individuals (End): 

23
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Knockdown of LsIRP1A+LsIRP1B (formerly called IRP1 and IRP2) in preadult female lice. Slightly shorter egg strings. Knockdown 62% for IRP1 and 44% for IRP2. Described in the fragment fields is only F3, see experiment A for F2.

A F4 (CopB2)

Phenotype CV: 

General

Experiment Batch ID: 

A

Experiment Contact: 

Christiane Trösse

Start Date: 

23 January, 2012 - 09:00

End Date: 

24 February, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>65%
Phenotype

Number of Individuals (End): 

5
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F3 (IRP2)

Phenotype CV: 

General

Experiment Batch ID: 

A

Experiment Contact: 

Christiane Trösse

Start Date: 

23 January, 2012 - 09:00

End Date: 

24 February, 2012 - 09:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

Gene or target symbol: 

LsIRP1B
Fragment description

Fragment length: 

441bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID: 

F3

Primer Group: 

b640

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>45%
Phenotype

Number of Individuals (End): 

23
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Knockdown of LsIRP1B (formerly called IRP2) in preadult female lice. Slightly shorter egg strings caused less offspring.

D F32/F33

Phenotype CV: 

General

Experiment Batch ID: 

d

Experiment Contact: 

Sussie Dalvin

Start Date: 

24 August, 2012 - 13:49

End Date: 

3 October, 2012 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Target description
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F

Primer Group: 

LS_TSC2-F_b872

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>30%
Phenotype

Number of Individuals (End): 

14
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

TSC1+2, short eggstrings (15853), not the best appetite jlkj jkljlkj jk kl lj l jklkj

D F33

Phenotype CV: 

General

Experiment Batch ID: 

d

Experiment Contact: 

Sussie Dalvin

Start Date: 

24 August, 2012 - 13:49

End Date: 

3 October, 2012 - 00:00
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

Gene or target symbol: 

g18363_TSC2
Fragment description

Fragment length: 

432bp

Fragment concentration: 

600ng/ul

Sense sequence: 

aaaatgggcattcagcgacttggttgattggcacaatgcttataactgtttctacgagctgttgtacccatattgcaatgagaaatggtgtatgtgatagatgttatttttattgcattcgagatggaactttaagtaaggatgatattactaaaaattcggatagaagaagacacatgtcggatttttactccggtacaaataagagtcagttatcaaacttaagaagtgataaggaacttcataggcacacttctctggagagaatggagtcttctggagatgattatagagtaaaaaaagtgctacctgaaacatgcagttgttggtgctctggctgggctgaaatttacattcggcgtccaagtggggatgtatcctggatgtgtagagtgcaaaatactcagttgcttagtgcgcatacaccctcaa

Fragment Label ID: 

F33

Primer Group: 

LS_TSC2-F_b872

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>72%
Phenotype

Number of Individuals (End): 

11
Upload

Image file: 

Gallery Image: 

Image Quality: 

0
No votes yet

Tsc2, a bit short eggstring TSC-2: Significant downregulation (but only with primers outside the dsRNA fragments, both tested)

Pages

Subscribe to RSS - female reproduction