RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
21 AR F249 EMLSAT00000010968 EMLSAT00000010968, EMLSAT00000010968-706815 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010968
PreAdult2 No visible phenotype
no visible phenotype
30/11 Lepeophtheirus salmonis 2 January, 2019 - 13:12
22 AS F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY
PreAdult2 30/14 Lepeophtheirus salmonis 21 November, 2018 - 11:14
23 AR F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 No visible phenotype
no visible phenotype
30/10 Lepeophtheirus salmonis 21 November, 2018 - 10:10
24 RNAi_AS 11369+12062 EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:09
25 RNAi_AS 11366+11369+10832 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:07
26 RNAi_AS 10832+11369 EMLSAG00000010832, EMLSAG00000010832-693598 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:04
27 RNAi_AS 11366+12062 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:58
28 AR F244 EMLSAT00000010957 EMLSAT00000010957, EMLSAT00000010957-706804 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010957
PreAdult2 No visible phenotype
no visible phenotype
30/15 Lepeophtheirus salmonis 21 November, 2018 - 09:50
29 LsCHS1 EMLSAT00000002853, EMLSAT00000002853-698700 (mRNA) Lepeophtheirus salmonis
LsCHS1
PreAdult1, PreAdult2 Cuticle disorder 60/29 Lepeophtheirus salmonis 19 June, 2018 - 16:56
30 EMLSAT00000007048 cop + infection EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid no visible phenotype
no visible phenotype
180/45 Lepeophtheirus salmonis 14 June, 2018 - 14:10

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