RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
21 AV F0 extra CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2 normal
no visible phenotype 		30/17 Lepeophtheirus salmonis 2 May, 2019 - 09:59
22 AU F0 male CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		Adult No visible phenotype
Control 		30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:56
23 AV F404 EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult reproduction, female reproduction, egg string deformation, no eggstrings 30/15 Lepeophtheirus salmonis F>50.00% 2 May, 2019 - 09:49
24 Heme peroxidase F415 EMLSAT00000013035, EMLSAT00000013035-708882 (mRNA) Lepeophtheirus salmonis, EMLSAT00000013034, EMLSAT00000013034-708881 (mRNA) Lepeophtheirus salmonis
hpx 		Adult 35 Lepeophtheirus salmonis 2 May, 2019 - 09:42
25 AV F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3 		PreAdult2 normal
no visible phenotype 		30/15 Lepeophtheirus salmonis 2 May, 2019 - 09:38
26 AU F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2, Adult No visible phenotype
Control 		30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:37
27 AR F385 EMLSAT00000007048 EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis
EMLSAG00000007048 		PreAdult2 No visible phenotype
no visible phenotype 		30/12 Lepeophtheirus salmonis 2 January, 2019 - 14:10
28 AR F249 EMLSAT00000010968 EMLSAT00000010968, EMLSAT00000010968-706815 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010968 		PreAdult2 No visible phenotype
no visible phenotype 		30/11 Lepeophtheirus salmonis 2 January, 2019 - 13:12
29 AS F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY 		PreAdult2 30/14 Lepeophtheirus salmonis 21 November, 2018 - 11:14
30 AS F384 EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis, EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis PreAdult2 reproduction, female reproduction, no eggstrings 30/21 Lepeophtheirus salmonis F>99.00% 21 November, 2018 - 11:10

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