RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
51 Nauplii I 5055-1 EMLSAG00000005055, EMLSAG00000005055-687821 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:51
52 beta FTZ-F1 EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis, EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis
beta FTZ-F1
Nauplius, Copepodid Developmental arrest
developmental defect, Molting, no molting
0 Lepeophtheirus salmonis F>50.00% 14 June, 2018 - 13:50
53 Nauplii I 3892-1 EMLSAG00000003892, EMLSAG00000003892-686658 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:49
54 Nauplii I 4663-2 EMLSAG00000004663, EMLSAG00000004663-687429 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:49
55 Nauplii I 4663-1 EMLSAG00000004663, EMLSAG00000004663-687429 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:48
56 Dead copepodid EMLSAT00000009839_Var1Frag1and2 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Nauplius1, Copepodid survival, reduced Lepeophtheirus salmonis 14 June, 2018 - 13:48
57 Nauplii I 1736-2 EMLSAG00000001736, EMLSAG00000001736-684502 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:47
58 Nauplii I 1736-1 EMLSAG00000001736, EMLSAG00000001736-684502 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:47
59 Nauplii I 6889-2 EMLSAG00000006889, EMLSAG00000006889-689655 (gene) Lepeophtheirus salmonis Nauplius I developmental defect Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:46
60 Nauplii I 4808-2 EMLSAG00000004808, EMLSAG00000004808-687574 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:43

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