RNAi

Search or browse a total of 379 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
31 AR F244 EMLSAT00000010957 EMLSAT00000010957, EMLSAT00000010957-706804 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010957
PreAdult2 No visible phenotype
no visible phenotype
30/15 Lepeophtheirus salmonis 21 November, 2018 - 09:50
32 AS F379 EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis
EMLSAT00000008518
PreAdult2 reproduction, female reproduction, abnormal egg strings 30/15 Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:46
33 10397&5417 KD, first eggstring EMLSAT00000010397, EMLSAT00000010397-706244 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005417, EMLSAT00000005417-701264 (mRNA) Lepeophtheirus salmonis
EMLSAT00000010397, EMLSAT00000005417
PreAdult2 10 Lepeophtheirus salmonis 16 August, 2018 - 13:17
34 LsCHS1 EMLSAT00000002853, EMLSAT00000002853-698700 (mRNA) Lepeophtheirus salmonis
LsCHS1
PreAdult1, PreAdult2 Cuticle disorder 60/29 Lepeophtheirus salmonis 19 June, 2018 - 16:56
35 ExpD EMLSAT00000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Adult, Adult no visible phenotype 50/50 Lepeophtheirus salmonis F>99.00% 14 June, 2018 - 14:17
36 EMLSAT00000007048 cop + infection EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis Nauplius1, Copepodid no visible phenotype
no visible phenotype
180/45 Lepeophtheirus salmonis 14 June, 2018 - 14:10
37 EMLSAT00000005299 cop + infection EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
Emc3
Nauplius1, chalimus and preadult no proper visible phenotype but maybe quicker deve
no visible phenotype
180/52 Lepeophtheirus salmonis 14 June, 2018 - 14:05
38 Nauplii I 7798-1 EMLSAG00000007798, EMLSAG00000007798-690564 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:54
39 Nauplii I 3059-1 EMLSAG00000003059, EMLSAG00000003059-685825 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:54
40 Nauplii I 392-1 EMLSAG00000000392, EMLSAG00000000392-683158 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:52

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