RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
31 AR F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 No visible phenotype
no visible phenotype
30/10 Lepeophtheirus salmonis 21 November, 2018 - 10:10
32 RNAi_AS 11369+12062 EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:09
33 RNAi_AS 11366+11369+10832 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:07
34 RNAi_AS 10832+11369 EMLSAG00000010832, EMLSAG00000010832-693598 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:04
35 AS F380 EMLSAT00000000454, EMLSAT00000000454-696301 (mRNA) Lepeophtheirus salmonis
EMLSAT00000000454
PreAdult2 reproduction, female reproduction, no eggstrings 30/20 Lepeophtheirus salmonis F>99.00% 21 November, 2018 - 09:59
36 RNAi_AS 11366+12062 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:58
37 AR F244 EMLSAT00000010957 EMLSAT00000010957, EMLSAT00000010957-706804 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010957
PreAdult2 No visible phenotype
no visible phenotype
30/15 Lepeophtheirus salmonis 21 November, 2018 - 09:50
38 AS F379 EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis
EMLSAT00000008518
PreAdult2 reproduction, female reproduction, abnormal egg strings 30/15 Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:46
39 AR F376 EMLSAT00000004347 EMLSAG00000004347, EMLSAG00000004347-687113 (gene) Lepeophtheirus salmonis
EMLSAG00000004347
PreAdult2 shorter eggstring 30/10 Lepeophtheirus salmonis 21 September, 2018 - 19:44
40 10397&5417 KD, first eggstring EMLSAT00000010397, EMLSAT00000010397-706244 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005417, EMLSAT00000005417-701264 (mRNA) Lepeophtheirus salmonis
EMLSAT00000010397, EMLSAT00000005417
PreAdult2 10 Lepeophtheirus salmonis 16 August, 2018 - 13:17

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