RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
91 AN F337 EMLSAT00000010162, EMLSAT00000010162-706009 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult 60 Lepeophtheirus salmonis F>0.00% 14 June, 2018 - 11:51
92 EMLSAT00000009839_Var1Frag1 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/5 Lepeophtheirus salmonis 14 June, 2018 - 11:48
93 EMLSAT00000001458 cop + infection EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
60 Lepeophtheirus salmonis 14 June, 2018 - 11:36
94 ExpH - EMLSAT000000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>95.00% 14 June, 2018 - 11:08
95 ExpH - EMLSAT000000006642 EMLSAT00000006642, EMLSAT00000006642-702489 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>98.00% 14 June, 2018 - 10:57
96 AL Hormone receptor 3 (HR3) EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
EMLSAG00000000733
PreAdult2 No eggstrings.
female reproduction, no eggstrings
30/17 Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 10:47
97 ExpH - EMLSAT000000010949 EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis
Trypsin
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>93.00% 14 June, 2018 - 10:26
98 AQ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 Control 30/5 Lepeophtheirus salmonis 6 June, 2018 - 09:56
99 F367 EMLSAT00000005299 nauplia EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005299
Nauplius1 200 Lepeophtheirus salmonis 29 May, 2018 - 15:16
100 AQ F375 EMLSAG00000008959 EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis
EMLSAG00000008959
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/5 Lepeophtheirus salmonis 29 May, 2018 - 14:58

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