RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
371 LsCHS1 EMLSAT00000002853, EMLSAT00000002853-698700 (mRNA) Lepeophtheirus salmonis
LsCHS1
PreAdult1, PreAdult2 Cuticle disorder 60/29 Lepeophtheirus salmonis 19 June, 2018 - 16:56
372 T F181 EMLSAG00000001530, EMLSAG00000001530-684296 (gene) Lepeophtheirus salmonis PreAdult2 All animals with short and abnormal egg strings, f 30/10 Lepeophtheirus salmonis 26 February, 2015 - 15:51
373 T F178 EMLSAT00000003607, EMLSAT00000003607-699454 (mRNA) Lepeophtheirus salmonis
3607
PreAdult2, Adult A few slightly abnormal 28/14 Lepeophtheirus salmonis F>0.00% 14 November, 2014 - 13:20
374 R F161 EMLSAG00000000580, EMLSAG00000000580-683346 (gene) Lepeophtheirus salmonis
UN38_580
PreAdult2, Adult Abnormal eggstrings in some individuals
female reproduction, abnormal egg strings
37/20 Lepeophtheirus salmonis F>99.00% 7 October, 2014 - 10:17
375 T F166 EMLSAT00000010071, EMLSAT00000010071-705918 (mRNA) Lepeophtheirus salmonis
UN50_10071
PreAdult2, Adult Abnormal
female reproduction, abnormal egg strings
30/19 Lepeophtheirus salmonis F>96.00% 14 November, 2014 - 13:26
376 D F29 EMLSAG00000006214, EMLSAG00000006214-688980 (gene) Lepeophtheirus salmonis
g2282_trehalose
PreAdult2 a bit short eggstrings (?), other wise normal.
female reproduction
36/12 Lepeophtheirus salmonis F>59.00% 6 March, 2013 - 14:49
377 AC F230 EMLSAG00000000504, EMLSAG00000000504-683270 (gene) Lepeophtheirus salmonis
504
Adult 31/24 Lepeophtheirus salmonis 31 March, 2016 - 14:29
378 AF F264 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis, EMLSAG00000012943, EMLSAG00000012943-695709 (gene) Lepeophtheirus salmonis, EMLSAG00000006733, EMLSAG00000006733-689499 (gene) Lepeophtheirus salmonis
441, 12943, 6733
PreAdult2 30/17 Lepeophtheirus salmonis 16 July, 2016 - 11:56
379 Rhabdovirus - No9 prod of virus free strains LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9
N protein LsRV-No9
Nauplius I, Adult 480 Lepeophtheirus salmonis F>97.00% 20 April, 2018 - 09:38
RNAi-mediated treatment of two vertically transmitted...
380 Rhabdovirus - No9+127 prod of virus free strain LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9, LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127
N protein LsRV-No9, N protein LsRV-No127
Nauplius I, Adult 480 Lepeophtheirus salmonis F>66.00% 20 April, 2018 - 09:55
RNAi-mediated treatment of two vertically transmitted...

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