RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
391 A F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39/5 Lepeophtheirus salmonis F>65.00% 29 April, 2015 - 15:44
RNA interference mediated knockdown of the KDEL receptor and...
392 A F3 (IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis
LsIRP1B
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>45.00% 29 April, 2015 - 15:16
Characterisation of iron regulatory protein 1A and 1B in the...
393 A F2 (IRP1) EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1A
PreAdult2, Adult 39/25 Lepeophtheirus salmonis F>54.00% 28 April, 2015 - 17:24
Characterisation of iron regulatory protein 1A and 1B in the...
394 AC F231 EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
Adult, Adult 30/25 Lepeophtheirus salmonis F>99.50% 12 April, 2016 - 11:31
395 AC F230 EMLSAG00000000504, EMLSAG00000000504-683270 (gene) Lepeophtheirus salmonis
504
Adult 31/24 Lepeophtheirus salmonis 31 March, 2016 - 14:29
396 AC F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/24 Lepeophtheirus salmonis F>99.91% 12 April, 2016 - 10:35
397 AC F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
Adult female reproduction, no eggstrings 31/21 Lepeophtheirus salmonis 31 March, 2016 - 09:56
398 AC F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult Control 30/28 Lepeophtheirus salmonis 22 March, 2016 - 09:10
399 AA F222 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis PreAdult1 female, PreAdult2 male female reproduction, no spermatophore 120 Lepeophtheirus salmonis 20 October, 2015 - 14:33
400 6502_2 Naupli EMLSAG00000006502, EMLSAG00000006502-689268 (gene) Lepeophtheirus salmonis
Proboscipedia frag2
Nauplius, Copepodid No visible phenotype
no visible phenotype
Lepeophtheirus salmonis 27 March, 2018 - 10:26

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