RNAi

Search or browse a total of 337 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
41 Functional characterization of a heme Peroxidase (Nauplia) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
Nauplius1, Copepodid Decreased swimming activity
Motility, reduced
400/400 Lepeophtheirus salmonis F>89.00% 21 February, 2017 - 10:04
Molecular characterization and functional analysis of a...
42 beta FTZ-F1 EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis, EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis
beta FTZ-F1
Nauplius, Copepodid Developmental arrest
developmental defect, Molting, no molting
0 Lepeophtheirus salmonis F>50.00% 14 June, 2018 - 13:50
43 F149 EMLSAG00000010881, EMLSAG00000010881-693647 (gene) Lepeophtheirus salmonis Nauplius1, Copepodid Development of gut affected
developmental defect, organ development, intestine
200/190 Lepeophtheirus salmonis F>80.00% 11 June, 2014 - 14:07
44 Functional characterization of a heme Peroxidase (female) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
PreAdult1, PreAdult2/Adult Fall off the fish
survival, reduced
42 Lepeophtheirus salmonis F>78.00% 16 February, 2017 - 17:49
Molecular characterization and functional analysis of a...
45 Functional characterization of a heme Peroxidase (male) F159 EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis
HPX1
padII/ad, Adult Fall off the fish
survival, reduced
42/1 Lepeophtheirus salmonis F>98.00% 21 February, 2017 - 09:56
Molecular characterization and functional analysis of a...
46 Mucin-like spermatophore wall protein 2 (AG F270) EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis
MLSWP2
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>80.00% 26 January, 2017 - 14:55
A novel gene-family involved in spermatophore generation in...
47 Mucin-like spermatophore wall protein 1 (AG F269) EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis
MLSWP1
adult male females missing eggstrings/producing no off-spring Lepeophtheirus salmonis F>95.00% 26 January, 2017 - 15:07
A novel gene-family involved in spermatophore generation in...
48 LsCHS1 EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis PreAdultI males In preAdultII: Could not complete molting or had t
Molting, developmental defect
60 Lepeophtheirus salmonis F>80.00% 7 May, 2018 - 23:13
49 E F42 EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis
g13677_Rheb
PreAdult2, Adult Low reproduction and most are missing blood in the
feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings
39/25 Lepeophtheirus salmonis F>60.00% 12 March, 2013 - 10:38
Molecular characterization and functional analysis of...
50 Z F214 EMLSAG00000000426, EMLSAG00000000426-683192 (gene) Lepeophtheirus salmonis
426
PreAdult2, Adult Many bad and short eggstrings.
female reproduction, shorter eggstrings
30/19 Lepeophtheirus salmonis 3 September, 2015 - 09:21

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