RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
101 AQ F372 EMLSAG00000011562 EMLSAT00000011562, EMLSAT00000011562-707409 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011562
PreAdult2 No visible phenotype
no visible phenotype
30/13 Lepeophtheirus salmonis 29 May, 2018 - 14:37
102 AQ F374 EMLSAG00000011382 EMLSAT00000011382, EMLSAT00000011382-707229 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011382
PreAdult2 No visible phenotype
no visible phenotype
30/9 Lepeophtheirus salmonis 29 May, 2018 - 14:02
103 AQ F373 EMLSAG00000004269 EMLSAT00000004269, EMLSAT00000004269-700116 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
30/7 Lepeophtheirus salmonis 29 May, 2018 - 13:55
104 AP F247 EMLSAG00000005706 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
31 Lepeophtheirus salmonis 29 May, 2018 - 13:13
105 AP F365 EMLSAG00000003179 EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis PreAdult2 slightly shorter gonade and abdominal segment 30 Lepeophtheirus salmonis F>47.00% 29 May, 2018 - 12:46
106 AP F367 EMLSAG00000005299 EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005299
PreAdult2 slightly shorter gonade and abdominal segment
reproduction, female reproduction, shorter eggstrings
30/24 Lepeophtheirus salmonis F>16.00% 29 May, 2018 - 12:27
107 AP F366 EMLSAT00000001458 EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
EMLSAG00000001458
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>77.00% 29 May, 2018 - 11:26
108 LsCHS1 EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis PreAdultI males In preAdultII: Could not complete molting or had t
Molting, developmental defect
60 Lepeophtheirus salmonis F>80.00% 7 May, 2018 - 23:13
109 Nauplii I 12262+22100+4547+12296+12453 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis, EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis Nauplius I reproductive defect as adults
reproduction
Lepeophtheirus salmonis 2 May, 2018 - 14:43
110 Nauplii I 3422 EMLSAG00000003422, EMLSAG00000003422-686188 (gene) Lepeophtheirus salmonis Nauplius I Lepeophtheirus salmonis 2 May, 2018 - 14:42

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